MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRN...

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Autores principales:	Maia Segura-Wang, Bertrand Grenier, Suzana Ilic, Ursula Ruczizka, Maximiliane Dippel, Moritz Bünger, Matthias Hackl, Veronika Nagl
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	deoxynivalenol mycotoxin microRNA next generation sequencing biomarker Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c767
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spelling	oai:doaj.org-article:fa709f9c58b74aaaabdaee3657e1c7672021-11-11T17:26:30ZMicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers10.3390/ijms2221120431422-00671661-6596https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c7672021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12043https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.Maia Segura-WangBertrand GrenierSuzana IlicUrsula RuczizkaMaximiliane DippelMoritz BüngerMatthias HacklVeronika NaglMDPI AGarticledeoxynivalenolmycotoxinmicroRNAnext generation sequencingbiomarkerBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12043, p 12043 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	deoxynivalenol mycotoxin microRNA next generation sequencing biomarker Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	deoxynivalenol mycotoxin microRNA next generation sequencing biomarker Biology (General) QH301-705.5 Chemistry QD1-999 Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
description	Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.
format	article
author	Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl
author_facet	Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl
author_sort	Maia Segura-Wang
title	MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
title_short	MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
title_full	MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
title_fullStr	MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
title_full_unstemmed	MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
title_sort	microrna expression profiling in porcine liver, jejunum and serum upon dietary don exposure reveals candidate toxicity biomarkers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c767
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MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

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