MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers
Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRN...
Guardado en:
Autores principales: | , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
MDPI AG
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c767 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:fa709f9c58b74aaaabdaee3657e1c767 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:fa709f9c58b74aaaabdaee3657e1c7672021-11-11T17:26:30ZMicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers10.3390/ijms2221120431422-00671661-6596https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c7672021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12043https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.Maia Segura-WangBertrand GrenierSuzana IlicUrsula RuczizkaMaximiliane DippelMoritz BüngerMatthias HacklVeronika NaglMDPI AGarticledeoxynivalenolmycotoxinmicroRNAnext generation sequencingbiomarkerBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12043, p 12043 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
deoxynivalenol mycotoxin microRNA next generation sequencing biomarker Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
deoxynivalenol mycotoxin microRNA next generation sequencing biomarker Biology (General) QH301-705.5 Chemistry QD1-999 Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
description |
Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins. |
format |
article |
author |
Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl |
author_facet |
Maia Segura-Wang Bertrand Grenier Suzana Ilic Ursula Ruczizka Maximiliane Dippel Moritz Bünger Matthias Hackl Veronika Nagl |
author_sort |
Maia Segura-Wang |
title |
MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
title_short |
MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
title_full |
MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
title_fullStr |
MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
title_full_unstemmed |
MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers |
title_sort |
microrna expression profiling in porcine liver, jejunum and serum upon dietary don exposure reveals candidate toxicity biomarkers |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/fa709f9c58b74aaaabdaee3657e1c767 |
work_keys_str_mv |
AT maiasegurawang micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT bertrandgrenier micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT suzanailic micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT ursularuczizka micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT maximilianedippel micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT moritzbunger micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT matthiashackl micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers AT veronikanagl micrornaexpressionprofilinginporcineliverjejunumandserumupondietarydonexposurerevealscandidatetoxicitybiomarkers |
_version_ |
1718432097372209152 |