Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.

<h4>Background</h4>Platelets play a fundamental role in pathological events underlying acute coronary syndrome (ACS). Because platelets do not have a nucleus, proteomics constitutes an optimal approach to follow platelet molecular events associated with the onset of the acute episode.<...

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Autores principales:	Andrés Fernández Parguiña, Lilian Grigorian-Shamajian, Rosa M Agra, Elvis Teijeira-Fernández, Isaac Rosa, Jana Alonso, Juan E Viñuela-Roldán, Ana Seoane, José Ramón González-Juanatey, Angel García
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa731d58d4fb4dd6a185ad445c6bfa45
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spelling	oai:doaj.org-article:fa731d58d4fb4dd6a185ad445c6bfa452021-11-18T07:03:18ZProteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.1932-620310.1371/journal.pone.0013404https://doaj.org/article/fa731d58d4fb4dd6a185ad445c6bfa452010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976234/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Platelets play a fundamental role in pathological events underlying acute coronary syndrome (ACS). Because platelets do not have a nucleus, proteomics constitutes an optimal approach to follow platelet molecular events associated with the onset of the acute episode.<h4>Methodology/principal findings</h4>We performed the first high-resolution two-dimensional gel electrophoresis-based proteome analysis of circulating platelets from patients with non-ST segment elevation ACS (NSTE-ACS). Proteins were identified by mass spectrometry and validations were by western blotting. Forty protein features (corresponding to 22 unique genes) were found to be differentially regulated between NSTE-ACS patients and matched controls with chronic ischemic cardiopathy. The number of differences decreased at day 5 (28) and 6 months after the acute event (5). Interestingly, a systems biology approach demonstrated that 16 of the 22 differentially regulated proteins identified are interconnected as part of a common network related to cell assembly and organization and cell morphology, processes very related to platelet activation. Indeed, 14 of those proteins are either signaling or cytoskeletal, and nine of them are known to participate in platelet activation by αIIbβ3 and/or GPVI receptors. Several of the proteins identified participate in platelet activation through post-translational modifications, as shown here for ILK, Src and Talin. Interestingly, the platelet-secreted glycoprotein SPARC was down-regulated in NSTE-ACS patients compared to stable controls, which is consistent with a secretion process from activated platelets.<h4>Conclusions/significance</h4>The present study provides novel information on platelet proteome changes associated with platelet activation in NSTE-ACS, highlighting the presence of proteins involved in platelet signaling. This investigation paves the way for future studies in the search for novel platelet-related biomarkers and drug targets in ACS.Andrés Fernández ParguiñaLilian Grigorian-ShamajianRosa M AgraElvis Teijeira-FernándezIsaac RosaJana AlonsoJuan E Viñuela-RoldánAna SeoaneJosé Ramón González-JuanateyAngel GarcíaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13404 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrés Fernández Parguiña Lilian Grigorian-Shamajian Rosa M Agra Elvis Teijeira-Fernández Isaac Rosa Jana Alonso Juan E Viñuela-Roldán Ana Seoane José Ramón González-Juanatey Angel García Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
description	<h4>Background</h4>Platelets play a fundamental role in pathological events underlying acute coronary syndrome (ACS). Because platelets do not have a nucleus, proteomics constitutes an optimal approach to follow platelet molecular events associated with the onset of the acute episode.<h4>Methodology/principal findings</h4>We performed the first high-resolution two-dimensional gel electrophoresis-based proteome analysis of circulating platelets from patients with non-ST segment elevation ACS (NSTE-ACS). Proteins were identified by mass spectrometry and validations were by western blotting. Forty protein features (corresponding to 22 unique genes) were found to be differentially regulated between NSTE-ACS patients and matched controls with chronic ischemic cardiopathy. The number of differences decreased at day 5 (28) and 6 months after the acute event (5). Interestingly, a systems biology approach demonstrated that 16 of the 22 differentially regulated proteins identified are interconnected as part of a common network related to cell assembly and organization and cell morphology, processes very related to platelet activation. Indeed, 14 of those proteins are either signaling or cytoskeletal, and nine of them are known to participate in platelet activation by αIIbβ3 and/or GPVI receptors. Several of the proteins identified participate in platelet activation through post-translational modifications, as shown here for ILK, Src and Talin. Interestingly, the platelet-secreted glycoprotein SPARC was down-regulated in NSTE-ACS patients compared to stable controls, which is consistent with a secretion process from activated platelets.<h4>Conclusions/significance</h4>The present study provides novel information on platelet proteome changes associated with platelet activation in NSTE-ACS, highlighting the presence of proteins involved in platelet signaling. This investigation paves the way for future studies in the search for novel platelet-related biomarkers and drug targets in ACS.
format	article
author	Andrés Fernández Parguiña Lilian Grigorian-Shamajian Rosa M Agra Elvis Teijeira-Fernández Isaac Rosa Jana Alonso Juan E Viñuela-Roldán Ana Seoane José Ramón González-Juanatey Angel García
author_facet	Andrés Fernández Parguiña Lilian Grigorian-Shamajian Rosa M Agra Elvis Teijeira-Fernández Isaac Rosa Jana Alonso Juan E Viñuela-Roldán Ana Seoane José Ramón González-Juanatey Angel García
author_sort	Andrés Fernández Parguiña
title	Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
title_short	Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
title_full	Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
title_fullStr	Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
title_full_unstemmed	Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
title_sort	proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/fa731d58d4fb4dd6a185ad445c6bfa45
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Proteins involved in platelet signaling are differentially regulated in acute coronary syndrome: a proteomic study.

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