Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle

Abstract Milk small extracellular vesicles (sEV) contain proteins that provide potential information of host physiology and immunology. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. In this study, we aimed to explore the proteomic profile o...

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Autores principales:	Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Yuko Kitamura, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa747610073e4c6fae0a4a7309151148
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spelling	oai:doaj.org-article:fa747610073e4c6fae0a4a73091511482021-12-02T10:44:08ZProteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle10.1038/s41598-021-82598-22045-2322https://doaj.org/article/fa747610073e4c6fae0a4a73091511482021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82598-2https://doaj.org/toc/2045-2322Abstract Milk small extracellular vesicles (sEV) contain proteins that provide potential information of host physiology and immunology. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. In this study, we aimed to explore the proteomic profile of milk sEV from BLV-infected cattle compared with those from uninfected cattle. Milk sEV were isolated from three BLV-infected and three uninfected cattle. Proteomic analysis was performed by using a comprehensive nanoLC-MS/MS method. Furthermore, gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to evaluate the candidates for uniquely or differentially expressed proteins in milk sEV from BLV-infected cattle. Proteomic analysis revealed a total of 1330 common proteins in milk sEV among BLV-infected cattle, whereas 118 proteins were uniquely expressed compared with those from uninfected cattle. Twenty-six proteins in milk sEV were differentially expressed proteins more than two-fold significant difference (p < 0.05) in BLV-infected cattle. GO and KEGG analyses indicated that the candidates for uniquely or differentially expressed proteins in milk sEV had been involved in diverse biological activities including metabolic processes, cellular processes, respond to stimulus, binding, catalytic activities, cancer pathways, focal adhesion, and so on. Taken together, the present findings provided a novel insight into the proteomes of milk sEV from BLV-infected cattle.Md. Matiur RahmanShigeo TakashimaYuji O. KamatariYassien BadrYuko KitamuraKaori ShimizuAyaka OkadaYasuo InoshimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Md. Matiur Rahman Shigeo Takashima Yuji O. Kamatari Yassien Badr Yuko Kitamura Kaori Shimizu Ayaka Okada Yasuo Inoshima Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
description	Abstract Milk small extracellular vesicles (sEV) contain proteins that provide potential information of host physiology and immunology. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. In this study, we aimed to explore the proteomic profile of milk sEV from BLV-infected cattle compared with those from uninfected cattle. Milk sEV were isolated from three BLV-infected and three uninfected cattle. Proteomic analysis was performed by using a comprehensive nanoLC-MS/MS method. Furthermore, gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to evaluate the candidates for uniquely or differentially expressed proteins in milk sEV from BLV-infected cattle. Proteomic analysis revealed a total of 1330 common proteins in milk sEV among BLV-infected cattle, whereas 118 proteins were uniquely expressed compared with those from uninfected cattle. Twenty-six proteins in milk sEV were differentially expressed proteins more than two-fold significant difference (p < 0.05) in BLV-infected cattle. GO and KEGG analyses indicated that the candidates for uniquely or differentially expressed proteins in milk sEV had been involved in diverse biological activities including metabolic processes, cellular processes, respond to stimulus, binding, catalytic activities, cancer pathways, focal adhesion, and so on. Taken together, the present findings provided a novel insight into the proteomes of milk sEV from BLV-infected cattle.
format	article
author	Md. Matiur Rahman Shigeo Takashima Yuji O. Kamatari Yassien Badr Yuko Kitamura Kaori Shimizu Ayaka Okada Yasuo Inoshima
author_facet	Md. Matiur Rahman Shigeo Takashima Yuji O. Kamatari Yassien Badr Yuko Kitamura Kaori Shimizu Ayaka Okada Yasuo Inoshima
author_sort	Md. Matiur Rahman
title	Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
title_short	Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
title_full	Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
title_fullStr	Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
title_full_unstemmed	Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
title_sort	proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa747610073e4c6fae0a4a7309151148
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Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle

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