Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture

Abstract Guiding cell culture via engineering extracellular microenvironment has attracted tremendous attention due to its appealing potentials in the repair, maintenance, and development of tissues or even whole organs. However, conventional biofabrication technologies are usually less productive i...

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Autores principales:	Xia Ouyang, Kunyu Zhang, Jushuai Wu, Dexter Siu-Hong Wong, Qian Feng, Liming Bian, A. Ping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa7839a9d3bc4a09920bcce3b27f30da
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spelling	oai:doaj.org-article:fa7839a9d3bc4a09920bcce3b27f30da2021-12-02T16:06:48ZOptical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture10.1038/s41598-017-08598-32045-2322https://doaj.org/article/fa7839a9d3bc4a09920bcce3b27f30da2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08598-3https://doaj.org/toc/2045-2322Abstract Guiding cell culture via engineering extracellular microenvironment has attracted tremendous attention due to its appealing potentials in the repair, maintenance, and development of tissues or even whole organs. However, conventional biofabrication technologies are usually less productive in fabricating microscale three-dimensional (3D) constructs because of the strident requirements in processing precision and complexity. Here we present an optical µ-printing technology to rapidly fabricate 3D microscaffold arrays for 3D cell culture and cell-scaffold interaction studies on a single chip. Arrays of 3D cubic microscaffolds with cubical sizes matching the single-cell size were fabricated to facilitate cell spreading on suspended microbeams so as to expose both apical and basal cell membranes. We further showed that the increasing of the cubical size of the microscaffolds led to enhanced spreading of the seeded human mesenchymal stem cells and activation of mechanosensing signaling, thereby promoting osteogenesis. Moreover, we demonstrated that the spatially selective modification of the surfaces of suspended beams with a bioactive coating (gelatin methacrylate) via an in-situ printing process allowed tailorable cell adhesion and spreading on the 3D microscaffolds.Xia OuyangKunyu ZhangJushuai WuDexter Siu-Hong WongQian FengLiming BianA. Ping ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xia Ouyang Kunyu Zhang Jushuai Wu Dexter Siu-Hong Wong Qian Feng Liming Bian A. Ping Zhang Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
description	Abstract Guiding cell culture via engineering extracellular microenvironment has attracted tremendous attention due to its appealing potentials in the repair, maintenance, and development of tissues or even whole organs. However, conventional biofabrication technologies are usually less productive in fabricating microscale three-dimensional (3D) constructs because of the strident requirements in processing precision and complexity. Here we present an optical µ-printing technology to rapidly fabricate 3D microscaffold arrays for 3D cell culture and cell-scaffold interaction studies on a single chip. Arrays of 3D cubic microscaffolds with cubical sizes matching the single-cell size were fabricated to facilitate cell spreading on suspended microbeams so as to expose both apical and basal cell membranes. We further showed that the increasing of the cubical size of the microscaffolds led to enhanced spreading of the seeded human mesenchymal stem cells and activation of mechanosensing signaling, thereby promoting osteogenesis. Moreover, we demonstrated that the spatially selective modification of the surfaces of suspended beams with a bioactive coating (gelatin methacrylate) via an in-situ printing process allowed tailorable cell adhesion and spreading on the 3D microscaffolds.
format	article
author	Xia Ouyang Kunyu Zhang Jushuai Wu Dexter Siu-Hong Wong Qian Feng Liming Bian A. Ping Zhang
author_facet	Xia Ouyang Kunyu Zhang Jushuai Wu Dexter Siu-Hong Wong Qian Feng Liming Bian A. Ping Zhang
author_sort	Xia Ouyang
title	Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
title_short	Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
title_full	Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
title_fullStr	Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
title_full_unstemmed	Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
title_sort	optical µ-printing of cellular-scale microscaffold arrays for 3d cell culture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fa7839a9d3bc4a09920bcce3b27f30da
work_keys_str_mv	AT xiaouyang opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT kunyuzhang opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT jushuaiwu opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT dextersiuhongwong opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT qianfeng opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT limingbian opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture AT apingzhang opticalμprintingofcellularscalemicroscaffoldarraysfor3dcellculture
_version_	1718384880890413056

Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture

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