Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

Abstract The intermolt crustacean Y-organ (YO) maintains a basal state mediated by pulsatile release of molt inhibiting hormone (MIH), a neuropeptide produced in the eyestalk ganglia, inhibiting YO ecdysteroidogenesis. Reduction of MIH results in YO activation and the animal enters premolt. In the c...

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Autores principales:	S. Shyamal, S. Das, A. Guruacharya, D. L. Mykles, D. S. Durica
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa7a0d8e9ba34e4db5ed99ac0c28545f
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spelling	oai:doaj.org-article:fa7a0d8e9ba34e4db5ed99ac0c28545f2021-12-02T15:08:00ZTranscriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway10.1038/s41598-018-25368-x2045-2322https://doaj.org/article/fa7a0d8e9ba34e4db5ed99ac0c28545f2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25368-xhttps://doaj.org/toc/2045-2322Abstract The intermolt crustacean Y-organ (YO) maintains a basal state mediated by pulsatile release of molt inhibiting hormone (MIH), a neuropeptide produced in the eyestalk ganglia, inhibiting YO ecdysteroidogenesis. Reduction of MIH results in YO activation and the animal enters premolt. In the crab, Gecarcinus lateralis, molting was induced by eyestalk ablation (ESA). ESA animals were injected with either rapamycin, an mTOR inhibitor, or DMSO vehicle at Day 0. YOs were harvested at 1, 3, and 7 days post-ESA and processed for high throughput RNA sequencing. ESA-induced increases in mRNA levels of mTOR signaling genes (e.g., mTOR, Rheb, TSC1/2, Raptor, Akt, and S6 kinase) declined following rapamycin treatment. In concert with mTOR inhibition, mRNA levels of ecdysteroid biosynthesis genes (e.g., Nvd, Spo, Sad, Dib, and Phm) were decreased and accompanied by a decrease in hemolymph ecdysteroid titer. By contrast, rapamycin increased the mRNA level of FKBP12, the rapamycin-binding protein, as well as the mRNA levels of genes associated with Wnt and insulin-like growth factor signaling pathways. Many MIH and transforming growth factor-β signaling genes were down regulated in ESA animals. These results indicate that mTOR activity either directly or indirectly controls transcription of genes that drive activation of the YO.S. ShyamalS. DasA. GuruacharyaD. L. MyklesD. S. DuricaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-17 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Shyamal S. Das A. Guruacharya D. L. Mykles D. S. Durica Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
description	Abstract The intermolt crustacean Y-organ (YO) maintains a basal state mediated by pulsatile release of molt inhibiting hormone (MIH), a neuropeptide produced in the eyestalk ganglia, inhibiting YO ecdysteroidogenesis. Reduction of MIH results in YO activation and the animal enters premolt. In the crab, Gecarcinus lateralis, molting was induced by eyestalk ablation (ESA). ESA animals were injected with either rapamycin, an mTOR inhibitor, or DMSO vehicle at Day 0. YOs were harvested at 1, 3, and 7 days post-ESA and processed for high throughput RNA sequencing. ESA-induced increases in mRNA levels of mTOR signaling genes (e.g., mTOR, Rheb, TSC1/2, Raptor, Akt, and S6 kinase) declined following rapamycin treatment. In concert with mTOR inhibition, mRNA levels of ecdysteroid biosynthesis genes (e.g., Nvd, Spo, Sad, Dib, and Phm) were decreased and accompanied by a decrease in hemolymph ecdysteroid titer. By contrast, rapamycin increased the mRNA level of FKBP12, the rapamycin-binding protein, as well as the mRNA levels of genes associated with Wnt and insulin-like growth factor signaling pathways. Many MIH and transforming growth factor-β signaling genes were down regulated in ESA animals. These results indicate that mTOR activity either directly or indirectly controls transcription of genes that drive activation of the YO.
format	article
author	S. Shyamal S. Das A. Guruacharya D. L. Mykles D. S. Durica
author_facet	S. Shyamal S. Das A. Guruacharya D. L. Mykles D. S. Durica
author_sort	S. Shyamal
title	Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
title_short	Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
title_full	Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
title_fullStr	Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
title_full_unstemmed	Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway
title_sort	transcriptomic analysis of crustacean molting gland (y-organ) regulation via the mtor signaling pathway
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fa7a0d8e9ba34e4db5ed99ac0c28545f
work_keys_str_mv	AT sshyamal transcriptomicanalysisofcrustaceanmoltingglandyorganregulationviathemtorsignalingpathway AT sdas transcriptomicanalysisofcrustaceanmoltingglandyorganregulationviathemtorsignalingpathway AT aguruacharya transcriptomicanalysisofcrustaceanmoltingglandyorganregulationviathemtorsignalingpathway AT dlmykles transcriptomicanalysisofcrustaceanmoltingglandyorganregulationviathemtorsignalingpathway AT dsdurica transcriptomicanalysisofcrustaceanmoltingglandyorganregulationviathemtorsignalingpathway
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Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

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