Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer

Abstract The collagen gel droplet-embedded drug sensitivity test (CD-DST) was revealed to be useful for predicting the effect of S-1 adjuvant chemotherapy for pancreatic ductal adenocarcinoma (PDAC). However, collection of an adequate number of PDAC cells is difficult due to the surrounding fibrobla...

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Autores principales:	Katsutaka Mitachi, Kyohei Ariake, Hiroki Shima, Satoko Sato, Takayuki Miura, Shimpei Maeda, Masaharu Ishida, Masamichi Mizuma, Hideo Ohtsuka, Takashi Kamei, Kazuhiko Igarashi, Michiaki Unno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa83f9a72d394814885790e89c076786
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spelling	oai:doaj.org-article:fa83f9a72d394814885790e89c0767862021-12-02T16:36:11ZNovel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer10.1038/s41598-021-86099-02045-2322https://doaj.org/article/fa83f9a72d394814885790e89c0767862021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86099-0https://doaj.org/toc/2045-2322Abstract The collagen gel droplet-embedded drug sensitivity test (CD-DST) was revealed to be useful for predicting the effect of S-1 adjuvant chemotherapy for pancreatic ductal adenocarcinoma (PDAC). However, collection of an adequate number of PDAC cells is difficult due to the surrounding fibroblasts. Thus, the aim of this study was to discover novel biomarkers to predict chemosensitivity based on the CD-DST results. Proteomics analysis was performed using liquid chromatography tandem mass spectrometry (LC–MS/MS). Candidate proteins were validated in patients with 5-FU CD-DST results via immunohistochemistry (IHC). The relationships between the candidate proteins and the effect of the adjuvant S-1 were investigated via IHC. Among the 2696 proteins extracted by LC–MS/MS, C1TC and SAHH could accurately predict the CD-DST results. Recurrence-free survival (RFS) was significantly improved in the IHC-positive group compared with the IHC-negative group in both factors. The negative group did not show a significant difference from the group that did not receive S-1. The double-positive group was associated with significantly prolonged RFS compared to the no adjuvant chemotherapy group. C1TC and SAHH have been shown to be useful biomarkers for predicting 5-FU sensitivity as a substitute for the CD-DST in adjuvant chemotherapy for PDAC.Katsutaka MitachiKyohei AriakeHiroki ShimaSatoko SatoTakayuki MiuraShimpei MaedaMasaharu IshidaMasamichi MizumaHideo OhtsukaTakashi KameiKazuhiko IgarashiMichiaki UnnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katsutaka Mitachi Kyohei Ariake Hiroki Shima Satoko Sato Takayuki Miura Shimpei Maeda Masaharu Ishida Masamichi Mizuma Hideo Ohtsuka Takashi Kamei Kazuhiko Igarashi Michiaki Unno Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
description	Abstract The collagen gel droplet-embedded drug sensitivity test (CD-DST) was revealed to be useful for predicting the effect of S-1 adjuvant chemotherapy for pancreatic ductal adenocarcinoma (PDAC). However, collection of an adequate number of PDAC cells is difficult due to the surrounding fibroblasts. Thus, the aim of this study was to discover novel biomarkers to predict chemosensitivity based on the CD-DST results. Proteomics analysis was performed using liquid chromatography tandem mass spectrometry (LC–MS/MS). Candidate proteins were validated in patients with 5-FU CD-DST results via immunohistochemistry (IHC). The relationships between the candidate proteins and the effect of the adjuvant S-1 were investigated via IHC. Among the 2696 proteins extracted by LC–MS/MS, C1TC and SAHH could accurately predict the CD-DST results. Recurrence-free survival (RFS) was significantly improved in the IHC-positive group compared with the IHC-negative group in both factors. The negative group did not show a significant difference from the group that did not receive S-1. The double-positive group was associated with significantly prolonged RFS compared to the no adjuvant chemotherapy group. C1TC and SAHH have been shown to be useful biomarkers for predicting 5-FU sensitivity as a substitute for the CD-DST in adjuvant chemotherapy for PDAC.
format	article
author	Katsutaka Mitachi Kyohei Ariake Hiroki Shima Satoko Sato Takayuki Miura Shimpei Maeda Masaharu Ishida Masamichi Mizuma Hideo Ohtsuka Takashi Kamei Kazuhiko Igarashi Michiaki Unno
author_facet	Katsutaka Mitachi Kyohei Ariake Hiroki Shima Satoko Sato Takayuki Miura Shimpei Maeda Masaharu Ishida Masamichi Mizuma Hideo Ohtsuka Takashi Kamei Kazuhiko Igarashi Michiaki Unno
author_sort	Katsutaka Mitachi
title	Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
title_short	Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
title_full	Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
title_fullStr	Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
title_full_unstemmed	Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer
title_sort	novel candidate factors predicting the effect of s-1 adjuvant chemotherapy of pancreatic cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa83f9a72d394814885790e89c076786
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Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer

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