Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets

Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for t...

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Autores principales:	Lu Zeng, Wei Jiang, Liqiang Liu, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	vitamin b2 monoclonal antibody ic-elisa lateral-flow immunochromatographic assay strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fa8474e991374c299caaae7d0aefa762
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spelling	oai:doaj.org-article:fa8474e991374c299caaae7d0aefa7622021-11-26T11:19:46ZDevelopment of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets0954-01051465-344310.1080/09540105.2017.1360257https://doaj.org/article/fa8474e991374c299caaae7d0aefa7622018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1360257https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for the rapid detection of vitamin B2. Following routine fusion and selection, the optimum monoclonal antibody against vitamin B2 was obtained. The 50% inhibitory concentration and limit of detection of ic-ELISA were 8.18 and 1.80 ng/mL, respectively. The cut-off value of the lateral-flow ICA strip was 50 ng/mL. The results revealed that our developed methods are suitable for the on-site detection and mass screening of vitamin B2 in food and pharmaceutical products.Lu ZengWei JiangLiqiang LiuShanshan SongHua KuangTaylor & Francis Grouparticlevitamin b2monoclonal antibodyic-elisalateral-flow immunochromatographic assay stripAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 121-132 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	vitamin b2 monoclonal antibody ic-elisa lateral-flow immunochromatographic assay strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	vitamin b2 monoclonal antibody ic-elisa lateral-flow immunochromatographic assay strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
description	Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for the rapid detection of vitamin B2. Following routine fusion and selection, the optimum monoclonal antibody against vitamin B2 was obtained. The 50% inhibitory concentration and limit of detection of ic-ELISA were 8.18 and 1.80 ng/mL, respectively. The cut-off value of the lateral-flow ICA strip was 50 ng/mL. The results revealed that our developed methods are suitable for the on-site detection and mass screening of vitamin B2 in food and pharmaceutical products.
format	article
author	Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang
author_facet	Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang
author_sort	Lu Zeng
title	Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
title_short	Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
title_full	Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
title_fullStr	Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
title_full_unstemmed	Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
title_sort	development of ic-elisa and lateral-flow immunochromatographic strip for detection of vitamin b2 in an energy drink and vitamin tablets
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/fa8474e991374c299caaae7d0aefa762
work_keys_str_mv	AT luzeng developmentoficelisaandlateralflowimmunochromatographicstripfordetectionofvitaminb2inanenergydrinkandvitamintablets AT weijiang developmentoficelisaandlateralflowimmunochromatographicstripfordetectionofvitaminb2inanenergydrinkandvitamintablets AT liqiangliu developmentoficelisaandlateralflowimmunochromatographicstripfordetectionofvitaminb2inanenergydrinkandvitamintablets AT shanshansong developmentoficelisaandlateralflowimmunochromatographicstripfordetectionofvitaminb2inanenergydrinkandvitamintablets AT huakuang developmentoficelisaandlateralflowimmunochromatographicstripfordetectionofvitaminb2inanenergydrinkandvitamintablets
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Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets

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