On-Chip Purification of Tetracyclines Based on Copper Ions Interaction

Antibiotics are widely used to both prevent and treat bacterial diseases as well as promote animal growth. This massive use leads to the presence of residual antibiotics in food with severe consequences for human health. Limitations and regulations on the tolerated amount of antibiotics in food have...

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Autores principales:	Lorenzo Lunelli, Martina Germanis, Lia Vanzetti, Roberta Tatti, Cristina Potrich, Cecilia Pederzolli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	tetracycline purification copper ions polymeric microdevice magnetic microbeads food safety Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/fa85454b384f4a4390f2da37386041ce
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spelling	oai:doaj.org-article:fa85454b384f4a4390f2da37386041ce2021-11-11T19:12:28ZOn-Chip Purification of Tetracyclines Based on Copper Ions Interaction10.3390/s212172361424-8220https://doaj.org/article/fa85454b384f4a4390f2da37386041ce2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/21/7236https://doaj.org/toc/1424-8220Antibiotics are widely used to both prevent and treat bacterial diseases as well as promote animal growth. This massive use leads to the presence of residual antibiotics in food with severe consequences for human health. Limitations and regulations on the tolerated amount of antibiotics in food have been introduced and analytical methods have been developed. The bioanalytical methods usually employed to detect antibiotic residues, however, are time-consuming, expensive and laboratory-based. Novel methods with improved rapidity, portability and cost that are easy-to-use and sustainable are therefore highly desirable. In the attempt to fulfill this need, a microfluidic system was set up herein for the purification and pre-concentration of tetracyclines from raw milk selected as the case-study. The system includes a polymeric microfluidic chip containing magnetic beads loaded with copper to exploit the preferential interaction of tetracycline with divalent ions. The microfluidic system was demonstrated to efficiently pre-concentrate tetracycline, oxytetracycline and chlortetracycline with similar performances and efficiently purify tetracycline from raw milk without any pre-treatment. The simplified method described in this paper could be easily integrated in a compact and portable device for the in-field detection of tetracyclines, with the economic advantage of preventing food wastes and guaranteeing food safety.Lorenzo LunelliMartina GermanisLia VanzettiRoberta TattiCristina PotrichCecilia PederzolliMDPI AGarticletetracycline purificationcopper ionspolymeric microdevicemagnetic microbeadsfood safetyChemical technologyTP1-1185ENSensors, Vol 21, Iss 7236, p 7236 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tetracycline purification copper ions polymeric microdevice magnetic microbeads food safety Chemical technology TP1-1185
spellingShingle	tetracycline purification copper ions polymeric microdevice magnetic microbeads food safety Chemical technology TP1-1185 Lorenzo Lunelli Martina Germanis Lia Vanzetti Roberta Tatti Cristina Potrich Cecilia Pederzolli On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
description	Antibiotics are widely used to both prevent and treat bacterial diseases as well as promote animal growth. This massive use leads to the presence of residual antibiotics in food with severe consequences for human health. Limitations and regulations on the tolerated amount of antibiotics in food have been introduced and analytical methods have been developed. The bioanalytical methods usually employed to detect antibiotic residues, however, are time-consuming, expensive and laboratory-based. Novel methods with improved rapidity, portability and cost that are easy-to-use and sustainable are therefore highly desirable. In the attempt to fulfill this need, a microfluidic system was set up herein for the purification and pre-concentration of tetracyclines from raw milk selected as the case-study. The system includes a polymeric microfluidic chip containing magnetic beads loaded with copper to exploit the preferential interaction of tetracycline with divalent ions. The microfluidic system was demonstrated to efficiently pre-concentrate tetracycline, oxytetracycline and chlortetracycline with similar performances and efficiently purify tetracycline from raw milk without any pre-treatment. The simplified method described in this paper could be easily integrated in a compact and portable device for the in-field detection of tetracyclines, with the economic advantage of preventing food wastes and guaranteeing food safety.
format	article
author	Lorenzo Lunelli Martina Germanis Lia Vanzetti Roberta Tatti Cristina Potrich Cecilia Pederzolli
author_facet	Lorenzo Lunelli Martina Germanis Lia Vanzetti Roberta Tatti Cristina Potrich Cecilia Pederzolli
author_sort	Lorenzo Lunelli
title	On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
title_short	On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
title_full	On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
title_fullStr	On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
title_full_unstemmed	On-Chip Purification of Tetracyclines Based on Copper Ions Interaction
title_sort	on-chip purification of tetracyclines based on copper ions interaction
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa85454b384f4a4390f2da37386041ce
work_keys_str_mv	AT lorenzolunelli onchippurificationoftetracyclinesbasedoncopperionsinteraction AT martinagermanis onchippurificationoftetracyclinesbasedoncopperionsinteraction AT liavanzetti onchippurificationoftetracyclinesbasedoncopperionsinteraction AT robertatatti onchippurificationoftetracyclinesbasedoncopperionsinteraction AT cristinapotrich onchippurificationoftetracyclinesbasedoncopperionsinteraction AT ceciliapederzolli onchippurificationoftetracyclinesbasedoncopperionsinteraction
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On-Chip Purification of Tetracyclines Based on Copper Ions Interaction

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