Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]

Bacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gra...

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Autores principales:	Patthanasak Rungsirivanich, Elvina Parlindungan, Paula M. O’Connor, Des Field, Jennifer Mahony, Narumol Thongwai, Douwe van Sinderen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	amylocyclicin bacteriocin biocontrol gene cluster Miang plipastatin Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fa87c892e8f642a5988fcbe15dbd3bdb
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spelling	oai:doaj.org-article:fa87c892e8f642a5988fcbe15dbd3bdb2021-11-30T19:46:09ZSimultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]1664-302X10.3389/fmicb.2021.789362https://doaj.org/article/fa87c892e8f642a5988fcbe15dbd3bdb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.789362/fullhttps://doaj.org/toc/1664-302XBacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea.Patthanasak RungsirivanichPatthanasak RungsirivanichElvina ParlindunganElvina ParlindunganPaula M. O’ConnorPaula M. O’ConnorDes FieldDes FieldJennifer MahonyJennifer MahonyNarumol ThongwaiNarumol ThongwaiDouwe van SinderenDouwe van SinderenFrontiers Media S.A.articleamylocyclicinbacteriocinbiocontrolgene clusterMiangplipastatinMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amylocyclicin bacteriocin biocontrol gene cluster Miang plipastatin Microbiology QR1-502
spellingShingle	amylocyclicin bacteriocin biocontrol gene cluster Miang plipastatin Microbiology QR1-502 Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
description	Bacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea.
format	article
author	Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen
author_facet	Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen
author_sort	Patthanasak Rungsirivanich
title	Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
title_short	Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
title_full	Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
title_fullStr	Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
title_full_unstemmed	Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
title_sort	simultaneous production of multiple antimicrobial compounds by bacillus velezensis ml122-2 isolated from assam tea leaf [camellia sinensis var. assamica (j.w.mast.) kitam.]
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fa87c892e8f642a5988fcbe15dbd3bdb
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Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]

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