Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats

Samuel Woldekidan,1 Abay Mulu,2 Wondwossen Ergetie,3 Frehiwot Teka,1 Asfaw Meressa,1 Ashenif Tadele,1 Abiy Abebe,1 Worku Gemechu,1 Negero Gemeda,1 Rekik Ashebir,1 Meron Sileshi,4 Yoseph Tolcha4 1Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa,...

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Autores principales:	Woldekidan S, Mulu A, Ergetie W, Teka F, Meressa A, Tadele A, Abebe A, Gemechu W, Gemeda N, Ashebir R, Sileshi M, Tolcha Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetes moringa stenopetala pancreatic β-cells alloxan monohydrate Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/fa92cdc74dbd45f690256e5f5266eb90
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spelling	oai:doaj.org-article:fa92cdc74dbd45f690256e5f5266eb902021-12-02T15:19:13ZEvaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats1178-7007https://doaj.org/article/fa92cdc74dbd45f690256e5f5266eb902021-01-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-antihyperglycemic-effect-of-extract-of-moringa-stenopeta-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Samuel Woldekidan,1 Abay Mulu,2 Wondwossen Ergetie,3 Frehiwot Teka,1 Asfaw Meressa,1 Ashenif Tadele,1 Abiy Abebe,1 Worku Gemechu,1 Negero Gemeda,1 Rekik Ashebir,1 Meron Sileshi,4 Yoseph Tolcha4 1Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 2Department of Anatomy, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pathology, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 4National Clinical Chemistry Reference Laboratory, Addis Ababa, EthiopiaCorrespondence: Samuel WoldekidanDirectorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, P.O. Box 1242, Addis Ababa, EthiopiaEmail woldekidans6@gmail.comBackground: Diabetes is a serious metabolic disorder with complications that result in significant morbidity and mortality. Current drugs used for diabetes therapy are not free from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of this study is to evaluate the antidiabetic effect of Moringa stenopetala (Baker f.) aqueous leaves extract.Methods: Thirty rats of weight 90– 150 gram were distributed to five groups (n= 6). Then labelled as diabetic control (DC), normal control (NC), extract treated (MS 250 and 500mg/kg), and glibenclamide treated (GL 5mg/kg). The experimental rats were induced by intra-peritoneal injection of Alloxan monohydrate at a dose of 180 mg/kg after dissolving in normal saline. Clinical biochemistry such as AST, ALT, ALP, urea, creatinine, and cholesterol, blood glucose level, histopathological and preliminary phytochemical screening were evaluated.Results: Phytochemical tests revealed the presence of different secondary metabolites. Alkaloid, flavonoid, tannin, saponin, phytosteroids, phenols and terpenoids. Moringa stenopetala (Baker f.) leaves aqueous extract (250 and 500mg/kg) improved the body weight of rats, showed remarkable reduction in blood glucose concentration (P< 0.05), and significantly decreased serum urea, creatinine, ALT, AST and ALP (P < 0.05). Levels of serum cholesterol remained unaltered in the experimental groups when compared with diabetic control. Histopathology of non-treated rats showed deterioration of insulin producing pancreas cells; nevertheless, β-cells restoration was observed due to administration of Moringa stenopetala (Baker f.) aqueous leaves extract.Conclusion: It is possible to conclude that oral administration of Moringa stenopetala (Baker f.) aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days showed beneficial effects on antihyperglycemia, improved body weight and Alloxan damaged pancreatic β-cells, and restored biochemical changes.Keywords: diabetes, Moringa stenopetala, pancreatic β-cells, alloxan monohydrateWoldekidan SMulu AErgetie WTeka FMeressa ATadele AAbebe AGemechu WGemeda NAshebir RSileshi MTolcha YDove Medical Pressarticlediabetesmoringa stenopetalapancreatic β-cellsalloxan monohydrateSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 185-192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes moringa stenopetala pancreatic β-cells alloxan monohydrate Specialties of internal medicine RC581-951
spellingShingle	diabetes moringa stenopetala pancreatic β-cells alloxan monohydrate Specialties of internal medicine RC581-951 Woldekidan S Mulu A Ergetie W Teka F Meressa A Tadele A Abebe A Gemechu W Gemeda N Ashebir R Sileshi M Tolcha Y Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
description	Samuel Woldekidan,1 Abay Mulu,2 Wondwossen Ergetie,3 Frehiwot Teka,1 Asfaw Meressa,1 Ashenif Tadele,1 Abiy Abebe,1 Worku Gemechu,1 Negero Gemeda,1 Rekik Ashebir,1 Meron Sileshi,4 Yoseph Tolcha4 1Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 2Department of Anatomy, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pathology, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 4National Clinical Chemistry Reference Laboratory, Addis Ababa, EthiopiaCorrespondence: Samuel WoldekidanDirectorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, P.O. Box 1242, Addis Ababa, EthiopiaEmail woldekidans6@gmail.comBackground: Diabetes is a serious metabolic disorder with complications that result in significant morbidity and mortality. Current drugs used for diabetes therapy are not free from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of this study is to evaluate the antidiabetic effect of Moringa stenopetala (Baker f.) aqueous leaves extract.Methods: Thirty rats of weight 90– 150 gram were distributed to five groups (n= 6). Then labelled as diabetic control (DC), normal control (NC), extract treated (MS 250 and 500mg/kg), and glibenclamide treated (GL 5mg/kg). The experimental rats were induced by intra-peritoneal injection of Alloxan monohydrate at a dose of 180 mg/kg after dissolving in normal saline. Clinical biochemistry such as AST, ALT, ALP, urea, creatinine, and cholesterol, blood glucose level, histopathological and preliminary phytochemical screening were evaluated.Results: Phytochemical tests revealed the presence of different secondary metabolites. Alkaloid, flavonoid, tannin, saponin, phytosteroids, phenols and terpenoids. Moringa stenopetala (Baker f.) leaves aqueous extract (250 and 500mg/kg) improved the body weight of rats, showed remarkable reduction in blood glucose concentration (P< 0.05), and significantly decreased serum urea, creatinine, ALT, AST and ALP (P < 0.05). Levels of serum cholesterol remained unaltered in the experimental groups when compared with diabetic control. Histopathology of non-treated rats showed deterioration of insulin producing pancreas cells; nevertheless, β-cells restoration was observed due to administration of Moringa stenopetala (Baker f.) aqueous leaves extract.Conclusion: It is possible to conclude that oral administration of Moringa stenopetala (Baker f.) aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days showed beneficial effects on antihyperglycemia, improved body weight and Alloxan damaged pancreatic β-cells, and restored biochemical changes.Keywords: diabetes, Moringa stenopetala, pancreatic β-cells, alloxan monohydrate
format	article
author	Woldekidan S Mulu A Ergetie W Teka F Meressa A Tadele A Abebe A Gemechu W Gemeda N Ashebir R Sileshi M Tolcha Y
author_facet	Woldekidan S Mulu A Ergetie W Teka F Meressa A Tadele A Abebe A Gemechu W Gemeda N Ashebir R Sileshi M Tolcha Y
author_sort	Woldekidan S
title	Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
title_short	Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
title_full	Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
title_fullStr	Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
title_full_unstemmed	Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats
title_sort	evaluation of antihyperglycemic effect of extract of moringa stenopetala (baker f.) aqueous leaves on alloxan-induced diabetic rats
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/fa92cdc74dbd45f690256e5f5266eb90
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Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats

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