Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance

Abstract This study aimed to determine if there is an association between dysbiosis and nonalcoholic fatty liver disease (NAFLD) independent of obesity and insulin resistance (IR). This is a prospective cross-sectional study assessing the intestinal microbiome (IM) of 39 adults with biopsy-proven NA...

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Autores principales:	Hannah E. Da Silva, Anastasia Teterina, Elena M. Comelli, Amel Taibi, Bianca M. Arendt, Sandra E. Fischer, Wendy Lou, Johane P. Allard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa9e437180ba44e48b0abbe5263a89e4
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spelling	oai:doaj.org-article:fa9e437180ba44e48b0abbe5263a89e42021-12-02T15:09:09ZNonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance10.1038/s41598-018-19753-92045-2322https://doaj.org/article/fa9e437180ba44e48b0abbe5263a89e42018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19753-9https://doaj.org/toc/2045-2322Abstract This study aimed to determine if there is an association between dysbiosis and nonalcoholic fatty liver disease (NAFLD) independent of obesity and insulin resistance (IR). This is a prospective cross-sectional study assessing the intestinal microbiome (IM) of 39 adults with biopsy-proven NAFLD (15 simple steatosis [SS]; 24 nonalcoholic steatohepatitis [NASH]) and 28 healthy controls (HC). IM composition (llumina MiSeq Platform) in NAFLD patients compared to HC were identified by two statistical methods (Metastats, Wilcoxon). Selected taxa was validated using quantitative PCR (qPCR). Metabolites in feces and serum were also analyzed. In NAFLD, 8 operational taxonomic units, 6 genera, 6 families and 2 phyla (Bacteroidetes, Firmicutes) were less abundant and; 1 genus (Lactobacillus) and 1 family (Lactobacillaceae) were more abundant compared to HC. Lower abundance in both NASH and SS patients compared to HC were confirmed by qPCR for Ruminococcus, Faecalibacterium prausnitzii and Coprococcus. No difference was found between NASH and SS. This lower abundance in NAFLD (NASH+SS) was independent of BMI and IR. NAFLD patients had higher concentrations of fecal propionate and isobutyric acid and serum 2-hydroxybutyrate and L-lactic acid. These findings suggest a potential role for a specific IM community and functional profile in the pathogenesis of NAFLD.Hannah E. Da SilvaAnastasia TeterinaElena M. ComelliAmel TaibiBianca M. ArendtSandra E. FischerWendy LouJohane P. AllardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hannah E. Da Silva Anastasia Teterina Elena M. Comelli Amel Taibi Bianca M. Arendt Sandra E. Fischer Wendy Lou Johane P. Allard Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
description	Abstract This study aimed to determine if there is an association between dysbiosis and nonalcoholic fatty liver disease (NAFLD) independent of obesity and insulin resistance (IR). This is a prospective cross-sectional study assessing the intestinal microbiome (IM) of 39 adults with biopsy-proven NAFLD (15 simple steatosis [SS]; 24 nonalcoholic steatohepatitis [NASH]) and 28 healthy controls (HC). IM composition (llumina MiSeq Platform) in NAFLD patients compared to HC were identified by two statistical methods (Metastats, Wilcoxon). Selected taxa was validated using quantitative PCR (qPCR). Metabolites in feces and serum were also analyzed. In NAFLD, 8 operational taxonomic units, 6 genera, 6 families and 2 phyla (Bacteroidetes, Firmicutes) were less abundant and; 1 genus (Lactobacillus) and 1 family (Lactobacillaceae) were more abundant compared to HC. Lower abundance in both NASH and SS patients compared to HC were confirmed by qPCR for Ruminococcus, Faecalibacterium prausnitzii and Coprococcus. No difference was found between NASH and SS. This lower abundance in NAFLD (NASH+SS) was independent of BMI and IR. NAFLD patients had higher concentrations of fecal propionate and isobutyric acid and serum 2-hydroxybutyrate and L-lactic acid. These findings suggest a potential role for a specific IM community and functional profile in the pathogenesis of NAFLD.
format	article
author	Hannah E. Da Silva Anastasia Teterina Elena M. Comelli Amel Taibi Bianca M. Arendt Sandra E. Fischer Wendy Lou Johane P. Allard
author_facet	Hannah E. Da Silva Anastasia Teterina Elena M. Comelli Amel Taibi Bianca M. Arendt Sandra E. Fischer Wendy Lou Johane P. Allard
author_sort	Hannah E. Da Silva
title	Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
title_short	Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
title_full	Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
title_fullStr	Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
title_full_unstemmed	Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
title_sort	nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fa9e437180ba44e48b0abbe5263a89e4
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Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance

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