Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients

The largest microbial aggregation in the human body exists in the gastrointestinal tract. The microbiota in the host gastrointestinal tract comprises a diverse ecosystem, and the intestinal microbiota plays a vital role in maintaining gut homeostasis. This study aims to examine whether the gut micr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hanan Alatawi, Mahmoud Mosli, Omar I. Saadah, Vito Annese, Rashad Al-Hindi, Marfat Alatawy, Hadba Al-Amrah, Dikhnah Alshehri, Ahmad Bahieldin, Sherif Edris
Formato:	article
Lenguaje:	EN
Publicado:	Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2021
Materias:	Inflammatory bowel disease non-responders microbiota Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/faa0e7d8cf884a3b8a24672167f05dc3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:faa0e7d8cf884a3b8a24672167f05dc3
record_format	dspace
spelling	oai:doaj.org-article:faa0e7d8cf884a3b8a24672167f05dc32021-11-11T17:17:10ZAttributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients10.17305/bjbms.2021.64361512-86011840-4812https://doaj.org/article/faa0e7d8cf884a3b8a24672167f05dc32021-11-01T00:00:00Zhttps://www.bjbms.org/ojs/index.php/bjbms/article/view/6436https://doaj.org/toc/1512-8601https://doaj.org/toc/1840-4812 The largest microbial aggregation in the human body exists in the gastrointestinal tract. The microbiota in the host gastrointestinal tract comprises a diverse ecosystem, and the intestinal microbiota plays a vital role in maintaining gut homeostasis. This study aims to examine whether the gut microbiota influences unresponsiveness to anti-TNF-α treatments in primary nonresponder patients, and consequently identify the responsible microbes as biomarkers of unresponsiveness. Stool samples were collected from a cohort of patients with an established diagnosis of IBD, either ulcerative colitis (UC) or Crohn’s disease (CD), following completion of the induction phase of anti TNF therapy. 16S rRNA sequencing analysis was used to examine the pattern of microbiota communities in fecal samples. The quality and quantity of fecal microbiota were compared in responder and primary nonresponder IBD patients following anti-TNF-α therapy. As per our hypothesis, a difference in gut microbiome composition between the two patient subgroups was observed. A decreased abundance of short-chain fatty acid (SCFA)-producing bacteria, including Anaerostipes, Coprococcus, Lachnospira, Roseburia, and Ruminococcus, was detected in non-responsive patients, which was the hallmark of dysbiosis. Biomarkers of dysbiosis that were identified as predictors of clinical nonresponse, included Klebsiella, Eubacteriaceae, RF32, Bifidobacterium_animalis, and Muribaculaceae—previously known as S24-7. Signature biomarkers showed dramatic alteration in the composition of gut microbiota in patients who demonstrated primary nonresponse to anti-TNF-α agents. Dysbiosis, with features including a dropped biodiversity, augmentation in opportunistic pathogenic microbiota, and a lack of SCFA-producing bacteria, is a prominent feature of the microbiome of primary nonresponders to anti-TNF-α therapy. Hanan AlatawiMahmoud MosliOmar I. SaadahVito AnneseRashad Al-HindiMarfat AlatawyHadba Al-AmrahDikhnah AlshehriAhmad BahieldinSherif EdrisAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaarticleInflammatory bowel diseasenon-respondersmicrobiotaMedicine (General)R5-920ENBosnian Journal of Basic Medical Sciences (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Inflammatory bowel disease non-responders microbiota Medicine (General) R5-920
spellingShingle	Inflammatory bowel disease non-responders microbiota Medicine (General) R5-920 Hanan Alatawi Mahmoud Mosli Omar I. Saadah Vito Annese Rashad Al-Hindi Marfat Alatawy Hadba Al-Amrah Dikhnah Alshehri Ahmad Bahieldin Sherif Edris Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
description	The largest microbial aggregation in the human body exists in the gastrointestinal tract. The microbiota in the host gastrointestinal tract comprises a diverse ecosystem, and the intestinal microbiota plays a vital role in maintaining gut homeostasis. This study aims to examine whether the gut microbiota influences unresponsiveness to anti-TNF-α treatments in primary nonresponder patients, and consequently identify the responsible microbes as biomarkers of unresponsiveness. Stool samples were collected from a cohort of patients with an established diagnosis of IBD, either ulcerative colitis (UC) or Crohn’s disease (CD), following completion of the induction phase of anti TNF therapy. 16S rRNA sequencing analysis was used to examine the pattern of microbiota communities in fecal samples. The quality and quantity of fecal microbiota were compared in responder and primary nonresponder IBD patients following anti-TNF-α therapy. As per our hypothesis, a difference in gut microbiome composition between the two patient subgroups was observed. A decreased abundance of short-chain fatty acid (SCFA)-producing bacteria, including Anaerostipes, Coprococcus, Lachnospira, Roseburia, and Ruminococcus, was detected in non-responsive patients, which was the hallmark of dysbiosis. Biomarkers of dysbiosis that were identified as predictors of clinical nonresponse, included Klebsiella, Eubacteriaceae, RF32, Bifidobacterium_animalis, and Muribaculaceae—previously known as S24-7. Signature biomarkers showed dramatic alteration in the composition of gut microbiota in patients who demonstrated primary nonresponse to anti-TNF-α agents. Dysbiosis, with features including a dropped biodiversity, augmentation in opportunistic pathogenic microbiota, and a lack of SCFA-producing bacteria, is a prominent feature of the microbiome of primary nonresponders to anti-TNF-α therapy.
format	article
author	Hanan Alatawi Mahmoud Mosli Omar I. Saadah Vito Annese Rashad Al-Hindi Marfat Alatawy Hadba Al-Amrah Dikhnah Alshehri Ahmad Bahieldin Sherif Edris
author_facet	Hanan Alatawi Mahmoud Mosli Omar I. Saadah Vito Annese Rashad Al-Hindi Marfat Alatawy Hadba Al-Amrah Dikhnah Alshehri Ahmad Bahieldin Sherif Edris
author_sort	Hanan Alatawi
title	Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
title_short	Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
title_full	Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
title_fullStr	Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
title_full_unstemmed	Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients
title_sort	attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-tnf-α agents in inflammatory bowel disease patients
publisher	Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
publishDate	2021
url	https://doaj.org/article/faa0e7d8cf884a3b8a24672167f05dc3
work_keys_str_mv	AT hananalatawi attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT mahmoudmosli attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT omarisaadah attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT vitoannese attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT rashadalhindi attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT marfatalatawy attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT hadbaalamrah attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT dikhnahalshehri attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT ahmadbahieldin attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients AT sherifedris attributesofintestinalmicrobiotacompositionandtheircorrelationwithclinicalprimarynonresponsetoantitnfaagentsininflammatoryboweldiseasepatients
_version_	1718432155982364672

Attributes of intestinal microbiota composition and their correlation with clinical primary nonresponse to anti-TNF-α agents in inflammatory bowel disease patients

Ejemplares similares