Exosomes cloak the virion to transmit Enterovirus 71 non-lytically
Enterovirus 71 (EV71) is a non-enveloped virus and it can be released from host cells through a traditional cytolytic manner. Now, we showed EV71 could be spread non-lytically between cells during early viral infection. In order to explain this phenomenon, we separated supernatant fluids of rhabdomy...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/faa3427a9152442aa3321d3dd9940494 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:faa3427a9152442aa3321d3dd9940494 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:faa3427a9152442aa3321d3dd99404942021-11-17T14:21:57ZExosomes cloak the virion to transmit Enterovirus 71 non-lytically2150-55942150-560810.1080/21505594.2019.1705022https://doaj.org/article/faa3427a9152442aa3321d3dd99404942020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2019.1705022https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Enterovirus 71 (EV71) is a non-enveloped virus and it can be released from host cells through a traditional cytolytic manner. Now, we showed EV71 could be spread non-lytically between cells during early viral infection. In order to explain this phenomenon, we separated supernatant fluids of rhabdomyosarcoma (RD) cells cultures infected with EV71 by isopycnic gradient centrifugation. Two populations of virus particles were morphology indistinguishable by transmission electron microscope (TEM). It showed that some EV71 particles were wrapped inside extracellular vesicles which were verified to be exosomes by immunoassay and morphologic analysis. In addition, exosomes containing viral RNA were shed in plasma of EV71-infected encephalitis in children. Our findings indicate that the “non-enveloped” EV71 virions could be wrapped within exosomes which promote their spread in the absence of cell lysis. Abbreviation: EV71: enterovirus 71; EXO: exosome; RD: rhabdomyosarcoma; TEM: transmission electron microscope; HFMD: hand, foot, and mouth disease; HIV: immunodeficiency virus; HCV: hepatitis C virus; HTLV: Human T-cell lymphotropic virus; HAV: hepatitis A virus; MOI: multiplicity of infection; EVs: extracellular vesicles; VP1: viral capsid protein 1; NTA: nanoparticle tracking analysis; CNS: central nervous systemJiaqi GuJing WuDaihua FangYang QiuXinran ZouXiaonan JiaYiqian YinLi ShenLingxiang MaoTaylor & Francis Grouparticleev71non-lyticallyexosomesdensityexo-ev71Infectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 32-38 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
ev71 non-lytically exosomes density exo-ev71 Infectious and parasitic diseases RC109-216 |
| spellingShingle |
ev71 non-lytically exosomes density exo-ev71 Infectious and parasitic diseases RC109-216 Jiaqi Gu Jing Wu Daihua Fang Yang Qiu Xinran Zou Xiaonan Jia Yiqian Yin Li Shen Lingxiang Mao Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| description |
Enterovirus 71 (EV71) is a non-enveloped virus and it can be released from host cells through a traditional cytolytic manner. Now, we showed EV71 could be spread non-lytically between cells during early viral infection. In order to explain this phenomenon, we separated supernatant fluids of rhabdomyosarcoma (RD) cells cultures infected with EV71 by isopycnic gradient centrifugation. Two populations of virus particles were morphology indistinguishable by transmission electron microscope (TEM). It showed that some EV71 particles were wrapped inside extracellular vesicles which were verified to be exosomes by immunoassay and morphologic analysis. In addition, exosomes containing viral RNA were shed in plasma of EV71-infected encephalitis in children. Our findings indicate that the “non-enveloped” EV71 virions could be wrapped within exosomes which promote their spread in the absence of cell lysis. Abbreviation: EV71: enterovirus 71; EXO: exosome; RD: rhabdomyosarcoma; TEM: transmission electron microscope; HFMD: hand, foot, and mouth disease; HIV: immunodeficiency virus; HCV: hepatitis C virus; HTLV: Human T-cell lymphotropic virus; HAV: hepatitis A virus; MOI: multiplicity of infection; EVs: extracellular vesicles; VP1: viral capsid protein 1; NTA: nanoparticle tracking analysis; CNS: central nervous system |
| format |
article |
| author |
Jiaqi Gu Jing Wu Daihua Fang Yang Qiu Xinran Zou Xiaonan Jia Yiqian Yin Li Shen Lingxiang Mao |
| author_facet |
Jiaqi Gu Jing Wu Daihua Fang Yang Qiu Xinran Zou Xiaonan Jia Yiqian Yin Li Shen Lingxiang Mao |
| author_sort |
Jiaqi Gu |
| title |
Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| title_short |
Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| title_full |
Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| title_fullStr |
Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| title_full_unstemmed |
Exosomes cloak the virion to transmit Enterovirus 71 non-lytically |
| title_sort |
exosomes cloak the virion to transmit enterovirus 71 non-lytically |
| publisher |
Taylor & Francis Group |
| publishDate |
2020 |
| url |
https://doaj.org/article/faa3427a9152442aa3321d3dd9940494 |
| work_keys_str_mv |
AT jiaqigu exosomescloaktheviriontotransmitenterovirus71nonlytically AT jingwu exosomescloaktheviriontotransmitenterovirus71nonlytically AT daihuafang exosomescloaktheviriontotransmitenterovirus71nonlytically AT yangqiu exosomescloaktheviriontotransmitenterovirus71nonlytically AT xinranzou exosomescloaktheviriontotransmitenterovirus71nonlytically AT xiaonanjia exosomescloaktheviriontotransmitenterovirus71nonlytically AT yiqianyin exosomescloaktheviriontotransmitenterovirus71nonlytically AT lishen exosomescloaktheviriontotransmitenterovirus71nonlytically AT lingxiangmao exosomescloaktheviriontotransmitenterovirus71nonlytically |
| _version_ |
1718425522587828224 |