Lower Spermatozoal PIWI-LIKE 1 and 2 Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF

The four human PIWI-LIKE gene family members PIWI-LIKE 1–4 play a pivotal role in stem cell maintenance and transposon repression in the human germline. Therefore, dysregulation of these genes negatively influences the genetic stability of the respective germ ce...

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Autores principales:	Maria Giebler, Thomas Greither, Diana Handke, Gregor Seliger, Hermann M. Behre
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	male infertility <i>PIWI-LIKE</i> genes spermatozoa ART fertilization rate Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/faa4e325556f431d8dd4b332b410abc1
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spelling	oai:doaj.org-article:faa4e325556f431d8dd4b332b410abc12021-11-11T16:48:27ZLower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF10.3390/ijms2221113201422-00671661-6596https://doaj.org/article/faa4e325556f431d8dd4b332b410abc12021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11320https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The four human <i>PIWI-LIKE</i> gene family members <i>PIWI-LIKE 1–4</i> play a pivotal role in stem cell maintenance and transposon repression in the human germline. Therefore, dysregulation of these genes negatively influences the genetic stability of the respective germ cell and subsequent development and maturation. Recently, we demonstrated that a lower <i>PIWI-LIKE 2</i> mRNA expression in ejaculated spermatozoa is more frequent in men with oligozoospermia. In this study, we analysed how <i>PIWI-LIKE 1–4</i> mRNA expression in ejaculated spermatozoa predicts ART outcome. From 160 IVF or ICSI cycles, portions of swim-up spermatozoa used for fertilization were collected, and the total RNA was isolated. <i>PIWI-LIKE 1–4</i> mRNA expression was measured by qPCR using TaqMan probes with GAPDH as a reference gene. <i>PIWI-LIKE 1</i> and <i>2</i> transcript levels in the spermatozoa of the swim-up fraction were positively correlated to each other (r<sub>S</sub> = 0.78; <i>p</i> < 0.001). Moreover, lower <i>PIWI-LIKE 2</i> mRNA levels, as well as lower <i>PIWI-LIKE 1</i> mRNA levels, in these spermatozoa were positively associated with a fertilization rate ≥ 50% in the respective ART cycles (<i>p</i> = 0.02 and <i>p</i> = 0.0499, Mann–Whitney <i>U</i>-Test). When separately analysing IVF and ICSI cycles, <i>PIWI-LIKE 1</i> and <i>2</i> transcript levels were only significantly associated to increased fertilization rates in IVF, yet not in ICSI cycles. Spermatozoal <i>PIWI-LIKE 3</i> and <i>4</i> transcript levels were not significantly associated to fertilization rates in ART cycles. In conclusion, lower levels of spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> mRNA levels are positively associated with a higher fertilization rate in IVF cycles.Maria GieblerThomas GreitherDiana HandkeGregor SeligerHermann M. BehreMDPI AGarticlemale infertility<i>PIWI-LIKE</i> genesspermatozoaARTfertilization rateBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11320, p 11320 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	male infertility <i>PIWI-LIKE</i> genes spermatozoa ART fertilization rate Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	male infertility <i>PIWI-LIKE</i> genes spermatozoa ART fertilization rate Biology (General) QH301-705.5 Chemistry QD1-999 Maria Giebler Thomas Greither Diana Handke Gregor Seliger Hermann M. Behre Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
description	The four human <i>PIWI-LIKE</i> gene family members <i>PIWI-LIKE 1–4</i> play a pivotal role in stem cell maintenance and transposon repression in the human germline. Therefore, dysregulation of these genes negatively influences the genetic stability of the respective germ cell and subsequent development and maturation. Recently, we demonstrated that a lower <i>PIWI-LIKE 2</i> mRNA expression in ejaculated spermatozoa is more frequent in men with oligozoospermia. In this study, we analysed how <i>PIWI-LIKE 1–4</i> mRNA expression in ejaculated spermatozoa predicts ART outcome. From 160 IVF or ICSI cycles, portions of swim-up spermatozoa used for fertilization were collected, and the total RNA was isolated. <i>PIWI-LIKE 1–4</i> mRNA expression was measured by qPCR using TaqMan probes with GAPDH as a reference gene. <i>PIWI-LIKE 1</i> and <i>2</i> transcript levels in the spermatozoa of the swim-up fraction were positively correlated to each other (r<sub>S</sub> = 0.78; <i>p</i> < 0.001). Moreover, lower <i>PIWI-LIKE 2</i> mRNA levels, as well as lower <i>PIWI-LIKE 1</i> mRNA levels, in these spermatozoa were positively associated with a fertilization rate ≥ 50% in the respective ART cycles (<i>p</i> = 0.02 and <i>p</i> = 0.0499, Mann–Whitney <i>U</i>-Test). When separately analysing IVF and ICSI cycles, <i>PIWI-LIKE 1</i> and <i>2</i> transcript levels were only significantly associated to increased fertilization rates in IVF, yet not in ICSI cycles. Spermatozoal <i>PIWI-LIKE 3</i> and <i>4</i> transcript levels were not significantly associated to fertilization rates in ART cycles. In conclusion, lower levels of spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> mRNA levels are positively associated with a higher fertilization rate in IVF cycles.
format	article
author	Maria Giebler Thomas Greither Diana Handke Gregor Seliger Hermann M. Behre
author_facet	Maria Giebler Thomas Greither Diana Handke Gregor Seliger Hermann M. Behre
author_sort	Maria Giebler
title	Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
title_short	Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
title_full	Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
title_fullStr	Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
title_full_unstemmed	Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF
title_sort	lower spermatozoal <i>piwi-like 1</i> and <i>2</i> transcript levels are significantly associated with higher fertilization rates in ivf
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/faa4e325556f431d8dd4b332b410abc1
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Lower Spermatozoal <i>PIWI-LIKE 1</i> and <i>2</i> Transcript Levels Are Significantly Associated with Higher Fertilization Rates in IVF

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