Pax5 mediates the transcriptional activation of the CD81 gene

Abstract CD81 is an integral membrane protein of the tetraspanin family and forms complexes with a variety of other cell surface membrane proteins. CD81 is involved in cell migration and B cell activation. However, the mechanism of the transcriptional regulation of the CD81 gene remains unclear. Her...

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Autores principales:	Kohei Hosokawa, Hanako Ishimaru, Tadashi Watanabe, Masahiro Fujimuro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/faa70a72fbc64dc280a66910b587ae60
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spelling	oai:doaj.org-article:faa70a72fbc64dc280a66910b587ae602021-11-28T12:18:33ZPax5 mediates the transcriptional activation of the CD81 gene10.1038/s41598-021-02082-92045-2322https://doaj.org/article/faa70a72fbc64dc280a66910b587ae602021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02082-9https://doaj.org/toc/2045-2322Abstract CD81 is an integral membrane protein of the tetraspanin family and forms complexes with a variety of other cell surface membrane proteins. CD81 is involved in cell migration and B cell activation. However, the mechanism of the transcriptional regulation of the CD81 gene remains unclear. Here, we revealed that CD81 transcriptional activation was required for binding of the transcription factor Pax5 at the Pax5-binding sequence (-54)GCGGGAC(-48) located upstream of the transcriptional start site (TSS) of the CD81 gene. The reporter assay showed that the DNA sequence between − 130 and − 39 bp upstream of the TSS of the CD81 gene had promoter activity for CD81 transcription. The DNA sequence between − 130 and − 39 bp upstream of TSS of CD81 harbors two potential Pax5-binding sequences (-87)GCGTGAG(-81) and (-54)GCGGGAC(-48). Reporter, electrophoresis mobility shift, and chromatin immunoprecipitation (ChIP) assays disclosed that Pax5 bound to the (-54)GCGGGAC(-48) in the promoter region of the CD81 gene in order to activate CD81 transcription. Pax5 overexpression increased the expression level of CD81 protein, while the Pax5-knockdown by shRNA decreased CD81 expression. Moreover, we found that the expression level of CD81 was positively correlated with Pax5 expression in human tumor cell lines. Because CD81 was reported to be involved in cell migration, we evaluated the effects of Pax5 overexpression by wound healing and transwell assays. The data showed that overexpression of either Pax5 or CD81 promoted the epithelial cell migration. Thus, our findings provide insights into the transcriptional mechanism of the CD81 gene through transcription factor Pax5.Kohei HosokawaHanako IshimaruTadashi WatanabeMasahiro FujimuroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kohei Hosokawa Hanako Ishimaru Tadashi Watanabe Masahiro Fujimuro Pax5 mediates the transcriptional activation of the CD81 gene
description	Abstract CD81 is an integral membrane protein of the tetraspanin family and forms complexes with a variety of other cell surface membrane proteins. CD81 is involved in cell migration and B cell activation. However, the mechanism of the transcriptional regulation of the CD81 gene remains unclear. Here, we revealed that CD81 transcriptional activation was required for binding of the transcription factor Pax5 at the Pax5-binding sequence (-54)GCGGGAC(-48) located upstream of the transcriptional start site (TSS) of the CD81 gene. The reporter assay showed that the DNA sequence between − 130 and − 39 bp upstream of the TSS of the CD81 gene had promoter activity for CD81 transcription. The DNA sequence between − 130 and − 39 bp upstream of TSS of CD81 harbors two potential Pax5-binding sequences (-87)GCGTGAG(-81) and (-54)GCGGGAC(-48). Reporter, electrophoresis mobility shift, and chromatin immunoprecipitation (ChIP) assays disclosed that Pax5 bound to the (-54)GCGGGAC(-48) in the promoter region of the CD81 gene in order to activate CD81 transcription. Pax5 overexpression increased the expression level of CD81 protein, while the Pax5-knockdown by shRNA decreased CD81 expression. Moreover, we found that the expression level of CD81 was positively correlated with Pax5 expression in human tumor cell lines. Because CD81 was reported to be involved in cell migration, we evaluated the effects of Pax5 overexpression by wound healing and transwell assays. The data showed that overexpression of either Pax5 or CD81 promoted the epithelial cell migration. Thus, our findings provide insights into the transcriptional mechanism of the CD81 gene through transcription factor Pax5.
format	article
author	Kohei Hosokawa Hanako Ishimaru Tadashi Watanabe Masahiro Fujimuro
author_facet	Kohei Hosokawa Hanako Ishimaru Tadashi Watanabe Masahiro Fujimuro
author_sort	Kohei Hosokawa
title	Pax5 mediates the transcriptional activation of the CD81 gene
title_short	Pax5 mediates the transcriptional activation of the CD81 gene
title_full	Pax5 mediates the transcriptional activation of the CD81 gene
title_fullStr	Pax5 mediates the transcriptional activation of the CD81 gene
title_full_unstemmed	Pax5 mediates the transcriptional activation of the CD81 gene
title_sort	pax5 mediates the transcriptional activation of the cd81 gene
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/faa70a72fbc64dc280a66910b587ae60
work_keys_str_mv	AT koheihosokawa pax5mediatesthetranscriptionalactivationofthecd81gene AT hanakoishimaru pax5mediatesthetranscriptionalactivationofthecd81gene AT tadashiwatanabe pax5mediatesthetranscriptionalactivationofthecd81gene AT masahirofujimuro pax5mediatesthetranscriptionalactivationofthecd81gene
_version_	1718408116442234880

Pax5 mediates the transcriptional activation of the CD81 gene

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