Systematic Characterization of <i>TCP</i> Gene Family in Four Cotton Species Revealed That <i>GhTCP62</i> Regulates Branching in <i>Arabidopsis</i>
TEOSINTE-BRANCHED1/CYCLOIDEA/PCF <i>(TCP</i>) transcription factors play an essential role in regulating various physiological and biochemical functions during plant growth. However, the function of <i>TCP</i> transcription factors in <i>G. hirsutum</i> has not ye...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/fab10468bac94acda9365b59a667e3f5 |
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| Sumario: | TEOSINTE-BRANCHED1/CYCLOIDEA/PCF <i>(TCP</i>) transcription factors play an essential role in regulating various physiological and biochemical functions during plant growth. However, the function of <i>TCP</i> transcription factors in <i>G. hirsutum</i> has not yet been studied. In this study, we performed genome-wide identification and correlation analysis of the <i>TCP</i> transcription factor family in <i>G. hirsutum</i>. We identified 72 non-redundant <i>GhTCP</i> genes and divided them into seven subfamilies, based on phylogenetic analysis. Most <i>GhTCP</i> genes in the same subfamily displayed similar exon and intron structures and featured highly conserved motif structures in their subfamily. Additionally, the pattern of chromosomal distribution demonstrated that <i>GhTCP</i> genes were unevenly distributed on 24 out of 26 chromosomes, and that fragment replication was the main replication event of <i>GhTCP</i> genes. In <i>TB1</i> sub-family genes, <i>GhTCP62</i> was highly expressed in the axillary buds, suggesting that <i>GhTCP62</i> significantly affected cotton branching. Additionally, subcellular localization results indicated that <i>GhTCP62</i> is located in the nucleus and possesses typical transcription factor characteristics. The overexpression of <i>GhTCP62</i> in <i>Arabidopsis</i> resulted in fewer rosette-leaf branches and cauline-leaf branches. Furthermore, the increased expression of <i>HB21</i> and <i>HB40</i> genes in <i>Arabidopsis</i> plants overexpressing <i>GhTCP62</i> suggests that <i>GhTCP62</i> may regulate branching by positively regulating <i>HB21</i> and <i>HB40</i>. |
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