Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation

Stem cell therapy is considered an important and innovative tool for applied research in andrology, especially in infertility; therefore, it was later adapted as potential therapeutic agents. This study aimed to evaluate rat mononuclear bone marrow cells' ability to recover testis cells in cycl...

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Autores principales:	Ahmed M. Mousbah, Mohamed E. Abd El-Samie, Mohamed A. Elbehery, Ismail, A. El-Nady
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	testes spermatogenesis cyclophosphamide stem cells infertility Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.118002 https://doaj.org/article/fabf202a4f274d28adb677d895e380d1
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spelling	oai:doaj.org-article:fabf202a4f274d28adb677d895e380d12021-12-02T17:04:17ZImmunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantationhttps://dx.doi.org/10.21608/javs.2020.1180021687-40722090-3308https://doaj.org/article/fabf202a4f274d28adb677d895e380d12020-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_118002.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Stem cell therapy is considered an important and innovative tool for applied research in andrology, especially in infertility; therefore, it was later adapted as potential therapeutic agents. This study aimed to evaluate rat mononuclear bone marrow cells' ability to recover testis cells in cyclophosphamide (CTX)-treated rats and to assess its effects on hormonal and histopathological changes. The bone marrow cells were harvested from femurs and tibias of rats and purified by a Histopaque gradient. Mononuclear bone marrow cells transplantation was performed by intravenous injection of cells in cyclophosphamide-treated animals. Three weeks after transplantation, blood samples were collected and analyzed for hormonal assay. In addition, the testes were collected for histological and histopathological determination. The results depicted that the serum levels of all tested hormones were significantly different among the three experimental groups G1 (control, healthy animals), G2 (CTX induced infertility and untreated) and G3 (CTX induced infertility and treated with stem cells). FSH and LH levels were significantly increased in G2 (CTX) compared to G1 and G3. Total and free testosterone levels were slightly higher in G3 compared to G2. Mononuclear bone marrow cell transplantation promoted cellular reorganization of the seminiferous epithelium. Also, spermatogenesis regeneration was improved. In conclusion, bone marrow stem cells can regenerate the damaged testicular elements and hence restore hormonal regulation in cyclophosphamide treated rat. Therefore, the treatment of male infertility and testosterone deficiency could be therapeutically treated by using stem cells.Ahmed M. MousbahMohamed E. Abd El-SamieMohamed A. ElbeheryIsmail, A. El-NadyEgyptian Society for Animal Managementarticletestesspermatogenesiscyclophosphamidestem cellsinfertilityZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 4, Pp 47-54 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	testes spermatogenesis cyclophosphamide stem cells infertility Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	testes spermatogenesis cyclophosphamide stem cells infertility Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ahmed M. Mousbah Mohamed E. Abd El-Samie Mohamed A. Elbehery Ismail, A. El-Nady Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
description	Stem cell therapy is considered an important and innovative tool for applied research in andrology, especially in infertility; therefore, it was later adapted as potential therapeutic agents. This study aimed to evaluate rat mononuclear bone marrow cells' ability to recover testis cells in cyclophosphamide (CTX)-treated rats and to assess its effects on hormonal and histopathological changes. The bone marrow cells were harvested from femurs and tibias of rats and purified by a Histopaque gradient. Mononuclear bone marrow cells transplantation was performed by intravenous injection of cells in cyclophosphamide-treated animals. Three weeks after transplantation, blood samples were collected and analyzed for hormonal assay. In addition, the testes were collected for histological and histopathological determination. The results depicted that the serum levels of all tested hormones were significantly different among the three experimental groups G1 (control, healthy animals), G2 (CTX induced infertility and untreated) and G3 (CTX induced infertility and treated with stem cells). FSH and LH levels were significantly increased in G2 (CTX) compared to G1 and G3. Total and free testosterone levels were slightly higher in G3 compared to G2. Mononuclear bone marrow cell transplantation promoted cellular reorganization of the seminiferous epithelium. Also, spermatogenesis regeneration was improved. In conclusion, bone marrow stem cells can regenerate the damaged testicular elements and hence restore hormonal regulation in cyclophosphamide treated rat. Therefore, the treatment of male infertility and testosterone deficiency could be therapeutically treated by using stem cells.
format	article
author	Ahmed M. Mousbah Mohamed E. Abd El-Samie Mohamed A. Elbehery Ismail, A. El-Nady
author_facet	Ahmed M. Mousbah Mohamed E. Abd El-Samie Mohamed A. Elbehery Ismail, A. El-Nady
author_sort	Ahmed M. Mousbah
title	Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
title_short	Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
title_full	Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
title_fullStr	Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
title_full_unstemmed	Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation
title_sort	immunohistochemistry and hormonal investigation for spermatogenesis restoration in degenerated testes of rats after bone marrow stem cells transplantation
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.118002 https://doaj.org/article/fabf202a4f274d28adb677d895e380d1
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Immunohistochemistry And Hormonal Investigation For Spermatogenesis Restoration In Degenerated Testes Of Rats After Bone Marrow Stem Cells Transplantation

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