How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae

Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thaís Almeida Corrêa, Jéssica Fiorotti, Emily Mesquita, Laura Nóbrega Meirelles, Mariana Guedes Camargo, Caio Junior Balduino Coutinho-Rodrigues, Allan Felipe Marciano, Vânia Rita Elias Pinheiro Bittencourt, Patrícia Silva Golo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biological control entomopathogenic fungi hemocytes ticks phagocytic activity phenoloxidase Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fac9101605ff43a599d823f25f3a1115
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fac9101605ff43a599d823f25f3a1115
record_format	dspace
spelling	oai:doaj.org-article:fac9101605ff43a599d823f25f3a11152021-11-25T18:06:05ZHow Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>10.3390/jof71109502309-608Xhttps://doaj.org/article/fac9101605ff43a599d823f25f3a11152021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/950https://doaj.org/toc/2309-608XDopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of <i>Metarhizium anisopliae</i> has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of <i>Rhipicephalus microplus</i> inoculated with <i>M. anisopliae</i> blastospores. Exogenous DA increased both ticks’ survival 72 h after <i>M. anisopliae</i> inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with <i>M. anisopliae</i> did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.Thaís Almeida CorrêaJéssica FiorottiEmily MesquitaLaura Nóbrega MeirellesMariana Guedes CamargoCaio Junior Balduino Coutinho-RodriguesAllan Felipe MarcianoVânia Rita Elias Pinheiro BittencourtPatrícia Silva GoloMDPI AGarticlebiological controlentomopathogenic fungihemocytesticksphagocytic activityphenoloxidaseBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 950, p 950 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biological control entomopathogenic fungi hemocytes ticks phagocytic activity phenoloxidase Biology (General) QH301-705.5
spellingShingle	biological control entomopathogenic fungi hemocytes ticks phagocytic activity phenoloxidase Biology (General) QH301-705.5 Thaís Almeida Corrêa Jéssica Fiorotti Emily Mesquita Laura Nóbrega Meirelles Mariana Guedes Camargo Caio Junior Balduino Coutinho-Rodrigues Allan Felipe Marciano Vânia Rita Elias Pinheiro Bittencourt Patrícia Silva Golo How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
description	Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of <i>Metarhizium anisopliae</i> has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of <i>Rhipicephalus microplus</i> inoculated with <i>M. anisopliae</i> blastospores. Exogenous DA increased both ticks’ survival 72 h after <i>M. anisopliae</i> inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with <i>M. anisopliae</i> did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.
format	article
author	Thaís Almeida Corrêa Jéssica Fiorotti Emily Mesquita Laura Nóbrega Meirelles Mariana Guedes Camargo Caio Junior Balduino Coutinho-Rodrigues Allan Felipe Marciano Vânia Rita Elias Pinheiro Bittencourt Patrícia Silva Golo
author_facet	Thaís Almeida Corrêa Jéssica Fiorotti Emily Mesquita Laura Nóbrega Meirelles Mariana Guedes Camargo Caio Junior Balduino Coutinho-Rodrigues Allan Felipe Marciano Vânia Rita Elias Pinheiro Bittencourt Patrícia Silva Golo
author_sort	Thaís Almeida Corrêa
title	How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
title_short	How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
title_full	How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
title_fullStr	How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
title_full_unstemmed	How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>
title_sort	how dopamine influences survival and cellular immune response of <i>rhipicephalus microplus</i> inoculated with <i>metarhizium anisopliae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fac9101605ff43a599d823f25f3a1115
work_keys_str_mv	AT thaisalmeidacorrea howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT jessicafiorotti howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT emilymesquita howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT lauranobregameirelles howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT marianaguedescamargo howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT caiojuniorbalduinocoutinhorodrigues howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT allanfelipemarciano howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT vaniaritaeliaspinheirobittencourt howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei AT patriciasilvagolo howdopamineinfluencessurvivalandcellularimmuneresponseofirhipicephalusmicroplusiinoculatedwithimetarhiziumanisopliaei
_version_	1718411658047520768

How Dopamine Influences Survival and Cellular Immune Response of <i>Rhipicephalus microplus</i> Inoculated with <i>Metarhizium anisopliae</i>

Ejemplares similares