Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model.
Cell lines are key tools in cancer research allowing the generation of neoplasias in animal models resembling the initial tumours able to mimic the original neoplasias closely in vivo. Canine lymphoma is the major hematopoietic malignancy in dogs and considered as a valuable spontaneous large animal...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/face307281f94a77a9ed9e2684719311 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:face307281f94a77a9ed9e2684719311 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:face307281f94a77a9ed9e26847193112021-11-18T07:14:04ZAuthentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model.1932-620310.1371/journal.pone.0040078https://doaj.org/article/face307281f94a77a9ed9e26847193112012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22761949/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cell lines are key tools in cancer research allowing the generation of neoplasias in animal models resembling the initial tumours able to mimic the original neoplasias closely in vivo. Canine lymphoma is the major hematopoietic malignancy in dogs and considered as a valuable spontaneous large animal model for human Non-Hodgkin's Lymphoma (NHL). Herein we describe the establishment and characterisation of an in vivo model using the canine B-cell lymphoma cell line CLBL-1 analysing the stability of the induced tumours and the ability to resemble the original material. CLBL-1 was injected into Rag2(-/-)γ(c) (-/-) mice. The generated tumor material was analysed by immunophenotyping and histopathology and used to establish the cell line CLBL-1M. Both cell lines were karyotyped for detection of chromosomal aberrations. Additionally, CLBL-1 was stimulated with IL-2 and DSP30 as described for primary canine B-cell lymphomas and NHL to examine the stimulatory effect on cell proliferation. CLBL-1 in vivo application resulted in lymphoma-like disease and tumor formation. Immunophenotypic analysis of tumorous material showed expression of CD45(+), MHCII(+), CD11a(+) and CD79αcy(+). PARR analysis showed positivity for IgH indicating a monoclonal character. These cytogenetic, molecular, immunophenotypical and histological characterisations of the in vivo model reveal that the induced tumours and thereof generated cell line resemble closely the original material. After DSP30 and IL-2 stimulation, CLBL-1 showed to respond in the same way as primary material. The herein described CLBL-1 in vivo model provides a highly stable tool for B-cell lymphoma research in veterinary and human medicine allowing various further in vivo studies.Barbara C RütgenSaskia WillenbrockNicola Reimann-BergIngrid WalterAndrea Fuchs-BaumgartingerSiegfried WagnerBoris KovacicSabine E EsslerIlse SchwendenweinIngo NolteArmin SaalmüllerHugo Murua EscobarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e40078 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Barbara C Rütgen Saskia Willenbrock Nicola Reimann-Berg Ingrid Walter Andrea Fuchs-Baumgartinger Siegfried Wagner Boris Kovacic Sabine E Essler Ilse Schwendenwein Ingo Nolte Armin Saalmüller Hugo Murua Escobar Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| description |
Cell lines are key tools in cancer research allowing the generation of neoplasias in animal models resembling the initial tumours able to mimic the original neoplasias closely in vivo. Canine lymphoma is the major hematopoietic malignancy in dogs and considered as a valuable spontaneous large animal model for human Non-Hodgkin's Lymphoma (NHL). Herein we describe the establishment and characterisation of an in vivo model using the canine B-cell lymphoma cell line CLBL-1 analysing the stability of the induced tumours and the ability to resemble the original material. CLBL-1 was injected into Rag2(-/-)γ(c) (-/-) mice. The generated tumor material was analysed by immunophenotyping and histopathology and used to establish the cell line CLBL-1M. Both cell lines were karyotyped for detection of chromosomal aberrations. Additionally, CLBL-1 was stimulated with IL-2 and DSP30 as described for primary canine B-cell lymphomas and NHL to examine the stimulatory effect on cell proliferation. CLBL-1 in vivo application resulted in lymphoma-like disease and tumor formation. Immunophenotypic analysis of tumorous material showed expression of CD45(+), MHCII(+), CD11a(+) and CD79αcy(+). PARR analysis showed positivity for IgH indicating a monoclonal character. These cytogenetic, molecular, immunophenotypical and histological characterisations of the in vivo model reveal that the induced tumours and thereof generated cell line resemble closely the original material. After DSP30 and IL-2 stimulation, CLBL-1 showed to respond in the same way as primary material. The herein described CLBL-1 in vivo model provides a highly stable tool for B-cell lymphoma research in veterinary and human medicine allowing various further in vivo studies. |
| format |
article |
| author |
Barbara C Rütgen Saskia Willenbrock Nicola Reimann-Berg Ingrid Walter Andrea Fuchs-Baumgartinger Siegfried Wagner Boris Kovacic Sabine E Essler Ilse Schwendenwein Ingo Nolte Armin Saalmüller Hugo Murua Escobar |
| author_facet |
Barbara C Rütgen Saskia Willenbrock Nicola Reimann-Berg Ingrid Walter Andrea Fuchs-Baumgartinger Siegfried Wagner Boris Kovacic Sabine E Essler Ilse Schwendenwein Ingo Nolte Armin Saalmüller Hugo Murua Escobar |
| author_sort |
Barbara C Rütgen |
| title |
Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| title_short |
Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| title_full |
Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| title_fullStr |
Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| title_full_unstemmed |
Authentication of primordial characteristics of the CLBL-1 cell line prove the integrity of a canine B-cell lymphoma in a murine in vivo model. |
| title_sort |
authentication of primordial characteristics of the clbl-1 cell line prove the integrity of a canine b-cell lymphoma in a murine in vivo model. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/face307281f94a77a9ed9e2684719311 |
| work_keys_str_mv |
AT barbaracrutgen authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT saskiawillenbrock authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT nicolareimannberg authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT ingridwalter authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT andreafuchsbaumgartinger authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT siegfriedwagner authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT boriskovacic authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT sabineeessler authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT ilseschwendenwein authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT ingonolte authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT arminsaalmuller authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel AT hugomuruaescobar authenticationofprimordialcharacteristicsoftheclbl1celllineprovetheintegrityofacaninebcelllymphomainamurineinvivomodel |
| _version_ |
1718423767018897408 |