Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Abstract The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Subjects having at least one implant with peri-implantitis, no diabetes, and not taki...

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Autores principales:	Hwey-Chin Yeh, Jang-Jih Lu, Shih-Cheng Chang, Mao-Cheng Ge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/facec06b584a4cbf84d7ece40576947f
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spelling	oai:doaj.org-article:facec06b584a4cbf84d7ece40576947f2021-12-02T15:09:29ZIdentification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry10.1038/s41598-018-37450-52045-2322https://doaj.org/article/facec06b584a4cbf84d7ece40576947f2019-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-37450-5https://doaj.org/toc/2045-2322Abstract The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Subjects having at least one implant with peri-implantitis, no diabetes, and not taking antibiotics in the previous 3 months were selected. Peri-implantitis was defined when surrounding bone loss ≥0.5 mm and bleeding on probing was found. Microbial samples were collected from peri-implantitis pockets using paper points. After incubation and isolation, the colonies were analyzed by MALDI-TOF MS. A total of 126 isolates were cultivated and identified from 12 samples, in identification rates of 82.5% at the species level and 12.72% at the genus level. Although the compositions were highly variable, major habitants in different peri-implant pockets could be identified. Among them the most distinguished were Neisseria flavescens (87%), Streptococcus constellatus (56%), Slackia exigua (46%), Streptococcus intermedius (45%), Fusobacterium nucleatum (45%) and Gemella morbillorum (43%). This preliminary study provides comprehensive and reliable data for future study designs involving MALDI-TOF MS and peri-implantitis in a more specific, easy, rapid and economical way. MALDI-TOF MS could be a new clinical method to evaluate and monitor oral microbiota associated with the disease.Hwey-Chin YehJang-Jih LuShih-Cheng ChangMao-Cheng GeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hwey-Chin Yeh Jang-Jih Lu Shih-Cheng Chang Mao-Cheng Ge Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
description	Abstract The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Subjects having at least one implant with peri-implantitis, no diabetes, and not taking antibiotics in the previous 3 months were selected. Peri-implantitis was defined when surrounding bone loss ≥0.5 mm and bleeding on probing was found. Microbial samples were collected from peri-implantitis pockets using paper points. After incubation and isolation, the colonies were analyzed by MALDI-TOF MS. A total of 126 isolates were cultivated and identified from 12 samples, in identification rates of 82.5% at the species level and 12.72% at the genus level. Although the compositions were highly variable, major habitants in different peri-implant pockets could be identified. Among them the most distinguished were Neisseria flavescens (87%), Streptococcus constellatus (56%), Slackia exigua (46%), Streptococcus intermedius (45%), Fusobacterium nucleatum (45%) and Gemella morbillorum (43%). This preliminary study provides comprehensive and reliable data for future study designs involving MALDI-TOF MS and peri-implantitis in a more specific, easy, rapid and economical way. MALDI-TOF MS could be a new clinical method to evaluate and monitor oral microbiota associated with the disease.
format	article
author	Hwey-Chin Yeh Jang-Jih Lu Shih-Cheng Chang Mao-Cheng Ge
author_facet	Hwey-Chin Yeh Jang-Jih Lu Shih-Cheng Chang Mao-Cheng Ge
author_sort	Hwey-Chin Yeh
title	Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_short	Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full	Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_fullStr	Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full_unstemmed	Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_sort	identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/facec06b584a4cbf84d7ece40576947f
work_keys_str_mv	AT hweychinyeh identificationofmicrobiotainperiimplantitispocketsbymatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT jangjihlu identificationofmicrobiotainperiimplantitispocketsbymatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT shihchengchang identificationofmicrobiotainperiimplantitispocketsbymatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT maochengge identificationofmicrobiotainperiimplantitispocketsbymatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry
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Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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