Genome-wide tracking of dCas9-methyltransferase footprints

Genome-wide tracking of dCas9-methyltransferase footprints

Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.

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Detalles Bibliográficos
Autores principales: Christina Galonska, Jocelyn Charlton, Alexandra L. Mattei, Julie Donaghey, Kendell Clement, Hongcang Gu, Arman W. Mohammad, Elena K. Stamenova, Davide Cacchiarelli, Sven Klages, Bernd Timmermann, Tobias Cantz, Hans R. Schöler, Andreas Gnirke, Michael J. Ziller, Alexander Meissner
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b4
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Descripción
Sumario:Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.

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