Genome-wide tracking of dCas9-methyltransferase footprints

Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.

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Auteurs principaux:	Christina Galonska, Jocelyn Charlton, Alexandra L. Mattei, Julie Donaghey, Kendell Clement, Hongcang Gu, Arman W. Mohammad, Elena K. Stamenova, Davide Cacchiarelli, Sven Klages, Bernd Timmermann, Tobias Cantz, Hans R. Schöler, Andreas Gnirke, Michael J. Ziller, Alexander Meissner
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b4
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spelling	oai:doaj.org-article:facfcb998fb94a91ab5dd5199cd3a1b42021-12-02T14:40:02ZGenome-wide tracking of dCas9-methyltransferase footprints10.1038/s41467-017-02708-52041-1723https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b42018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02708-5https://doaj.org/toc/2041-1723Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.Christina GalonskaJocelyn CharltonAlexandra L. MatteiJulie DonagheyKendell ClementHongcang GuArman W. MohammadElena K. StamenovaDavide CacchiarelliSven KlagesBernd TimmermannTobias CantzHans R. SchölerAndreas GnirkeMichael J. ZillerAlexander MeissnerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christina Galonska Jocelyn Charlton Alexandra L. Mattei Julie Donaghey Kendell Clement Hongcang Gu Arman W. Mohammad Elena K. Stamenova Davide Cacchiarelli Sven Klages Bernd Timmermann Tobias Cantz Hans R. Schöler Andreas Gnirke Michael J. Ziller Alexander Meissner Genome-wide tracking of dCas9-methyltransferase footprints
description	Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.
format	article
author	Christina Galonska Jocelyn Charlton Alexandra L. Mattei Julie Donaghey Kendell Clement Hongcang Gu Arman W. Mohammad Elena K. Stamenova Davide Cacchiarelli Sven Klages Bernd Timmermann Tobias Cantz Hans R. Schöler Andreas Gnirke Michael J. Ziller Alexander Meissner
author_facet	Christina Galonska Jocelyn Charlton Alexandra L. Mattei Julie Donaghey Kendell Clement Hongcang Gu Arman W. Mohammad Elena K. Stamenova Davide Cacchiarelli Sven Klages Bernd Timmermann Tobias Cantz Hans R. Schöler Andreas Gnirke Michael J. Ziller Alexander Meissner
author_sort	Christina Galonska
title	Genome-wide tracking of dCas9-methyltransferase footprints
title_short	Genome-wide tracking of dCas9-methyltransferase footprints
title_full	Genome-wide tracking of dCas9-methyltransferase footprints
title_fullStr	Genome-wide tracking of dCas9-methyltransferase footprints
title_full_unstemmed	Genome-wide tracking of dCas9-methyltransferase footprints
title_sort	genome-wide tracking of dcas9-methyltransferase footprints
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b4
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Genome-wide tracking of dCas9-methyltransferase footprints

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