Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice

Abstract The present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model of dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelia...

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Autores principales:	Yuan-Chieh Lee, Li-Yi Sun, Jia-Rong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fad1547e947d424ba639f755438e5aa9
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spelling	oai:doaj.org-article:fad1547e947d424ba639f755438e5aa92021-11-14T12:22:11ZProtective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice10.1038/s41598-021-01503-z2045-2322https://doaj.org/article/fad1547e947d424ba639f755438e5aa92021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01503-zhttps://doaj.org/toc/2045-2322Abstract The present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model of dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelial tight junction with microvilli, and decreased conjunctival goblet cells. Topical application of adipose-derived stem cell-conditioned medium (ADSC-CM) stimulated lacrimal tear secretion, preserved tight junction and microvilli of the corneal epithelium, and increased the density of goblet cells and MUC16 expression in the conjunctiva. The low-molecular-weight fractions (< 10 kDa and < 3 kDa) of ADSC-CM (LADSC-CM) provided better protections than the > 10 kDa or > 3 kDa fractions of ADSC-CM. In the in vitro study, desiccation for 10 min or hyperosmolarity (490 osmols) for 24 h caused decreased viability of human corneal epithelial cells, which were reversed by LADSC-CM. The active ingredients in the LADSC-CM were lipophobic and stable after heating and lyophilization. Our study demonstrated that LADSC-CM had beneficial effects on experimental dry eye. It is worthy of further exploration for the active ingredient(s) and the mechanism.Yuan-Chieh LeeLi-Yi SunJia-Rong ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuan-Chieh Lee Li-Yi Sun Jia-Rong Zhang Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
description	Abstract The present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model of dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelial tight junction with microvilli, and decreased conjunctival goblet cells. Topical application of adipose-derived stem cell-conditioned medium (ADSC-CM) stimulated lacrimal tear secretion, preserved tight junction and microvilli of the corneal epithelium, and increased the density of goblet cells and MUC16 expression in the conjunctiva. The low-molecular-weight fractions (< 10 kDa and < 3 kDa) of ADSC-CM (LADSC-CM) provided better protections than the > 10 kDa or > 3 kDa fractions of ADSC-CM. In the in vitro study, desiccation for 10 min or hyperosmolarity (490 osmols) for 24 h caused decreased viability of human corneal epithelial cells, which were reversed by LADSC-CM. The active ingredients in the LADSC-CM were lipophobic and stable after heating and lyophilization. Our study demonstrated that LADSC-CM had beneficial effects on experimental dry eye. It is worthy of further exploration for the active ingredient(s) and the mechanism.
format	article
author	Yuan-Chieh Lee Li-Yi Sun Jia-Rong Zhang
author_facet	Yuan-Chieh Lee Li-Yi Sun Jia-Rong Zhang
author_sort	Yuan-Chieh Lee
title	Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
title_short	Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
title_full	Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
title_fullStr	Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
title_full_unstemmed	Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
title_sort	protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fad1547e947d424ba639f755438e5aa9
work_keys_str_mv	AT yuanchiehlee protectiveeffectsoflowmolecularweightcomponentsofadiposestemcellderivedconditionedmediumondryeyesyndromeinmice AT liyisun protectiveeffectsoflowmolecularweightcomponentsofadiposestemcellderivedconditionedmediumondryeyesyndromeinmice AT jiarongzhang protectiveeffectsoflowmolecularweightcomponentsofadiposestemcellderivedconditionedmediumondryeyesyndromeinmice
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Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice

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