Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance

Cadmium (Cd), a readily absorbed and translocated toxic heavy metal, inhibits plant growth, interrupts metabolic homeostasis and induces oxidative damage. Responses towards Cd-stress differ among plant cultivars, and the complex integrated relationships between Cd accumulation, detoxification mechan...

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Autores principales:	Aya Mahmoud, Hamada AbdElgawad, Badreldin A. Hamed, Gerrit T.S. Beemster, Nadia M. El-Shafey
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Cd maize detoxification oxidative stress superoxide dismutase Cd-tolerance mechanisms Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/fad5f244d63e467ebed33c401fd3cba6
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spelling	oai:doaj.org-article:fad5f244d63e467ebed33c401fd3cba62021-11-25T16:28:56ZDifferences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance10.3390/antiox101118122076-3921https://doaj.org/article/fad5f244d63e467ebed33c401fd3cba62021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1812https://doaj.org/toc/2076-3921Cadmium (Cd), a readily absorbed and translocated toxic heavy metal, inhibits plant growth, interrupts metabolic homeostasis and induces oxidative damage. Responses towards Cd-stress differ among plant cultivars, and the complex integrated relationships between Cd accumulation, detoxification mechanisms and antioxidant defenses still need to be unraveled. To this end, 12 Egyptian maize cultivars were grown under Cd-stress to test their Cd-stress tolerance. Out of these cultivars, tolerant (TWC360 and TWC321), moderately sensitive (TWC324) and sensitive (SC128) cultivars were selected, and we determined their response to Cd in terms of biomass, Cd accumulation and antioxidant defense system. The reduction in biomass was highly obvious in sensitive cultivars, while TWC360 and TWC321 showed high Cd-tolerance. The cultivar TWC321 showed lower Cd uptake concurrently with an enhanced antioxidant defense system. Interestingly, TWC360 accumulated more Cd in the shoot, accompanied with increased Cd detoxification and sequestration. A principal component analysis revealed a clear separation between the sensitive and tolerant cultivars with significance of the antioxidant defenses, including superoxide dismutase (SOD). To confirm the involvement of SOD in Cd-tolerance, we studied the effect of Cd-stress on a transgenic maize line (TG) constitutively overexpressing <i>AtFeSOD</i> gene in comparison to its wild type (WT). Compared to their WT, the TG plants showed less Cd accumulation and improved growth, physiology, antioxidant and detoxification systems. These results demonstrate the role of SOD in determining Cd-tolerance.Aya MahmoudHamada AbdElgawadBadreldin A. HamedGerrit T.S. BeemsterNadia M. El-ShafeyMDPI AGarticleCdmaizedetoxificationoxidative stresssuperoxide dismutaseCd-tolerance mechanismsTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1812, p 1812 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cd maize detoxification oxidative stress superoxide dismutase Cd-tolerance mechanisms Therapeutics. Pharmacology RM1-950
spellingShingle	Cd maize detoxification oxidative stress superoxide dismutase Cd-tolerance mechanisms Therapeutics. Pharmacology RM1-950 Aya Mahmoud Hamada AbdElgawad Badreldin A. Hamed Gerrit T.S. Beemster Nadia M. El-Shafey Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
description	Cadmium (Cd), a readily absorbed and translocated toxic heavy metal, inhibits plant growth, interrupts metabolic homeostasis and induces oxidative damage. Responses towards Cd-stress differ among plant cultivars, and the complex integrated relationships between Cd accumulation, detoxification mechanisms and antioxidant defenses still need to be unraveled. To this end, 12 Egyptian maize cultivars were grown under Cd-stress to test their Cd-stress tolerance. Out of these cultivars, tolerant (TWC360 and TWC321), moderately sensitive (TWC324) and sensitive (SC128) cultivars were selected, and we determined their response to Cd in terms of biomass, Cd accumulation and antioxidant defense system. The reduction in biomass was highly obvious in sensitive cultivars, while TWC360 and TWC321 showed high Cd-tolerance. The cultivar TWC321 showed lower Cd uptake concurrently with an enhanced antioxidant defense system. Interestingly, TWC360 accumulated more Cd in the shoot, accompanied with increased Cd detoxification and sequestration. A principal component analysis revealed a clear separation between the sensitive and tolerant cultivars with significance of the antioxidant defenses, including superoxide dismutase (SOD). To confirm the involvement of SOD in Cd-tolerance, we studied the effect of Cd-stress on a transgenic maize line (TG) constitutively overexpressing <i>AtFeSOD</i> gene in comparison to its wild type (WT). Compared to their WT, the TG plants showed less Cd accumulation and improved growth, physiology, antioxidant and detoxification systems. These results demonstrate the role of SOD in determining Cd-tolerance.
format	article
author	Aya Mahmoud Hamada AbdElgawad Badreldin A. Hamed Gerrit T.S. Beemster Nadia M. El-Shafey
author_facet	Aya Mahmoud Hamada AbdElgawad Badreldin A. Hamed Gerrit T.S. Beemster Nadia M. El-Shafey
author_sort	Aya Mahmoud
title	Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
title_short	Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
title_full	Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
title_fullStr	Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
title_full_unstemmed	Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance
title_sort	differences in cadmium accumulation, detoxification and antioxidant defenses between contrasting maize cultivars implicate a role of superoxide dismutase in cd tolerance
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fad5f244d63e467ebed33c401fd3cba6
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Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance

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