Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide

The marine diatom Phaeodactylum tricornutum is an important basal resource in the marine food chain and is used as a standard test organism in toxicological studies. In this study, in vivo experiments were performed to analyze the effects of 1-octyl-3-methylimidazolium bromide ([C8mim]Br) on the gro...

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Autores principales:	XY Deng, J Cheng, XL Hu, K Gao, CH Wang
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Biology (General) QH301-705.5 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fad943775aa7447eba879b19f3823524
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spelling	oai:doaj.org-article:fad943775aa7447eba879b19f38235242021-11-18T09:18:33ZPhysiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide1864-77821864-779010.3354/ab00643https://doaj.org/article/fad943775aa7447eba879b19f38235242015-12-01T00:00:00Zhttps://www.int-res.com/abstracts/ab/v24/n2/p109-115/https://doaj.org/toc/1864-7782https://doaj.org/toc/1864-7790The marine diatom Phaeodactylum tricornutum is an important basal resource in the marine food chain and is used as a standard test organism in toxicological studies. In this study, in vivo experiments were performed to analyze the effects of 1-octyl-3-methylimidazolium bromide ([C8mim]Br) on the growth, photosynthetic activity, and antioxidant enzymes of P. tricornutum using 96 h growth tests in a batch-culture system. The results showed that [C8mim]Br significantly inhibited the growth of P. tricornutum, with a 96 h EC50 of 8.89 mg l-1, and likely restricts PSII electron transfer and light use efficiency of the diatom. With increasing [C8mim]Br concentrations, the soluble protein content in the diatom increased by 35.9, 58.5, 123.3, 197.7, and 207.0% in 5, 10, 20, 40, and 80 l-1 [C8mim]Br treatments relative to the controls, respectively. Concentrations of [C8mim]Br ≤10 mg l-1 caused a slight increase of superoxide dismutase (SOD) activity (from 16.37 to 23.04 U g-1 protein) in the diatom, but inhibited its activity at concentrations above 10 mg l-1. These observations indicate that moderate [C8mim]Br stress (about 10 mg l-1) likely stimulates the synthesis of proteins and free radical quenching. The general increase in malondialdehyde (MDA) content suggests that the physiological effects of [C8mim]Br were caused by free radical generation. Thus, potential risks exist if [C8mim]Br is accidentally released into the aquatic environment.XY DengJ ChengXL HuK GaoCH WangInter-ResearcharticleBiology (General)QH301-705.5MicrobiologyQR1-502ENAquatic Biology, Vol 24, Iss 2, Pp 109-115 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Microbiology QR1-502
spellingShingle	Biology (General) QH301-705.5 Microbiology QR1-502 XY Deng J Cheng XL Hu K Gao CH Wang Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
description	The marine diatom Phaeodactylum tricornutum is an important basal resource in the marine food chain and is used as a standard test organism in toxicological studies. In this study, in vivo experiments were performed to analyze the effects of 1-octyl-3-methylimidazolium bromide ([C8mim]Br) on the growth, photosynthetic activity, and antioxidant enzymes of P. tricornutum using 96 h growth tests in a batch-culture system. The results showed that [C8mim]Br significantly inhibited the growth of P. tricornutum, with a 96 h EC50 of 8.89 mg l-1, and likely restricts PSII electron transfer and light use efficiency of the diatom. With increasing [C8mim]Br concentrations, the soluble protein content in the diatom increased by 35.9, 58.5, 123.3, 197.7, and 207.0% in 5, 10, 20, 40, and 80 l-1 [C8mim]Br treatments relative to the controls, respectively. Concentrations of [C8mim]Br ≤10 mg l-1 caused a slight increase of superoxide dismutase (SOD) activity (from 16.37 to 23.04 U g-1 protein) in the diatom, but inhibited its activity at concentrations above 10 mg l-1. These observations indicate that moderate [C8mim]Br stress (about 10 mg l-1) likely stimulates the synthesis of proteins and free radical quenching. The general increase in malondialdehyde (MDA) content suggests that the physiological effects of [C8mim]Br were caused by free radical generation. Thus, potential risks exist if [C8mim]Br is accidentally released into the aquatic environment.
format	article
author	XY Deng J Cheng XL Hu K Gao CH Wang
author_facet	XY Deng J Cheng XL Hu K Gao CH Wang
author_sort	XY Deng
title	Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
title_short	Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
title_full	Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
title_fullStr	Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
title_full_unstemmed	Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
title_sort	physiological and biochemical responses of a marine diatom phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/fad943775aa7447eba879b19f3823524
work_keys_str_mv	AT xydeng physiologicalandbiochemicalresponsesofamarinediatomphaeodactylumtricornutumexposedto1octyl3methylimidazoliumbromide AT jcheng physiologicalandbiochemicalresponsesofamarinediatomphaeodactylumtricornutumexposedto1octyl3methylimidazoliumbromide AT xlhu physiologicalandbiochemicalresponsesofamarinediatomphaeodactylumtricornutumexposedto1octyl3methylimidazoliumbromide AT kgao physiologicalandbiochemicalresponsesofamarinediatomphaeodactylumtricornutumexposedto1octyl3methylimidazoliumbromide AT chwang physiologicalandbiochemicalresponsesofamarinediatomphaeodactylumtricornutumexposedto1octyl3methylimidazoliumbromide
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Physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum exposed to 1-octyl-3-methylimidazolium bromide

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