Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs

Abstract Organoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this stud...

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Autores principales:	Mie Naruse, Masako Ochiai, Shigeki Sekine, Hirokazu Taniguchi, Teruhiko Yoshida, Hitoshi Ichikawa, Hiromi Sakamoto, Takashi Kubo, Kenji Matsumoto, Atsushi Ochiai, Toshio Imai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fad9475292c8447090559084e9f331ec
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spelling	oai:doaj.org-article:fad9475292c8447090559084e9f331ec2021-12-02T11:50:30ZRe-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs10.1038/s41598-021-81475-22045-2322https://doaj.org/article/fad9475292c8447090559084e9f331ec2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81475-2https://doaj.org/toc/2045-2322Abstract Organoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this study, we established organoids and paired cancer-associated fibroblasts (CAFs) from surgical specimens of colorectal carcinomas (CRCs), and evaluated gene expression profiles in organoids with and without co-culture with CAFs to assess interactions between tumor cells and CAFs in tumor tissues. We found that the expression levels of several genes, which are highly expressed in original CRC tissues, were downregulated in organoids but re-expressed in organoids by co-culturing with CAFs. They comprised immune response- and external stimulus-related genes, e.g., REG family and dual oxidases (DUOXs), which are known to have malignant functions, leading tumor cells to proliferative and/or anti-apoptotic states and drug resistant phenotypes. In addition, the degree of differential induction of REG1 and DUOX2 in the co-culture system varied depending on CAFs from each CRC case. In conclusion, the co-culture system of CRC organoids with paired CAFs was able to partially reproduce the tumor microenvironment.Mie NaruseMasako OchiaiShigeki SekineHirokazu TaniguchiTeruhiko YoshidaHitoshi IchikawaHiromi SakamotoTakashi KuboKenji MatsumotoAtsushi OchiaiToshio ImaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mie Naruse Masako Ochiai Shigeki Sekine Hirokazu Taniguchi Teruhiko Yoshida Hitoshi Ichikawa Hiromi Sakamoto Takashi Kubo Kenji Matsumoto Atsushi Ochiai Toshio Imai Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
description	Abstract Organoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this study, we established organoids and paired cancer-associated fibroblasts (CAFs) from surgical specimens of colorectal carcinomas (CRCs), and evaluated gene expression profiles in organoids with and without co-culture with CAFs to assess interactions between tumor cells and CAFs in tumor tissues. We found that the expression levels of several genes, which are highly expressed in original CRC tissues, were downregulated in organoids but re-expressed in organoids by co-culturing with CAFs. They comprised immune response- and external stimulus-related genes, e.g., REG family and dual oxidases (DUOXs), which are known to have malignant functions, leading tumor cells to proliferative and/or anti-apoptotic states and drug resistant phenotypes. In addition, the degree of differential induction of REG1 and DUOX2 in the co-culture system varied depending on CAFs from each CRC case. In conclusion, the co-culture system of CRC organoids with paired CAFs was able to partially reproduce the tumor microenvironment.
format	article
author	Mie Naruse Masako Ochiai Shigeki Sekine Hirokazu Taniguchi Teruhiko Yoshida Hitoshi Ichikawa Hiromi Sakamoto Takashi Kubo Kenji Matsumoto Atsushi Ochiai Toshio Imai
author_facet	Mie Naruse Masako Ochiai Shigeki Sekine Hirokazu Taniguchi Teruhiko Yoshida Hitoshi Ichikawa Hiromi Sakamoto Takashi Kubo Kenji Matsumoto Atsushi Ochiai Toshio Imai
author_sort	Mie Naruse
title	Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
title_short	Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
title_full	Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
title_fullStr	Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
title_full_unstemmed	Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs
title_sort	re-expression of reg family and duoxs genes in crc organoids by co-culturing with cafs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fad9475292c8447090559084e9f331ec
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Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs

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