Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects

Abstract Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work po...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexey A. Polilov, Anastasia A. Makarova, Song Pang, C. Shan Xu, Harald Hess
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fad983f034784938a9b6c455e89ebce6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fad983f034784938a9b6c455e89ebce6
record_format	dspace
spelling	oai:doaj.org-article:fad983f034784938a9b6c455e89ebce62021-12-02T13:20:12ZProtocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects10.1038/s41598-021-83936-02045-2322https://doaj.org/article/fad983f034784938a9b6c455e89ebce62021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83936-0https://doaj.org/toc/2045-2322Abstract Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work poorly with heterogeneous (consisting of tissues different in structure and in chemical composition) samples and require expensive equipment and usually much time. We have developed a simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy. This protocol, combined with focused ion-beam scanning electron microscopy, makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels. The protocol provides new opportunities for many areas of study, including connectomics.Alexey A. PolilovAnastasia A. MakarovaSong PangC. Shan XuHarald HessNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexey A. Polilov Anastasia A. Makarova Song Pang C. Shan Xu Harald Hess Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
description	Abstract Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work poorly with heterogeneous (consisting of tissues different in structure and in chemical composition) samples and require expensive equipment and usually much time. We have developed a simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy. This protocol, combined with focused ion-beam scanning electron microscopy, makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels. The protocol provides new opportunities for many areas of study, including connectomics.
format	article
author	Alexey A. Polilov Anastasia A. Makarova Song Pang C. Shan Xu Harald Hess
author_facet	Alexey A. Polilov Anastasia A. Makarova Song Pang C. Shan Xu Harald Hess
author_sort	Alexey A. Polilov
title	Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
title_short	Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
title_full	Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
title_fullStr	Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
title_full_unstemmed	Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects
title_sort	protocol for preparation of heterogeneous biological samples for 3d electron microscopy: a case study for insects
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fad983f034784938a9b6c455e89ebce6
work_keys_str_mv	AT alexeyapolilov protocolforpreparationofheterogeneousbiologicalsamplesfor3delectronmicroscopyacasestudyforinsects AT anastasiaamakarova protocolforpreparationofheterogeneousbiologicalsamplesfor3delectronmicroscopyacasestudyforinsects AT songpang protocolforpreparationofheterogeneousbiologicalsamplesfor3delectronmicroscopyacasestudyforinsects AT cshanxu protocolforpreparationofheterogeneousbiologicalsamplesfor3delectronmicroscopyacasestudyforinsects AT haraldhess protocolforpreparationofheterogeneousbiologicalsamplesfor3delectronmicroscopyacasestudyforinsects
_version_	1718393249063763968

Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects

Ejemplares similares