miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of choleste...

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Autores principales:	Lihui Yao, Wenjing Zhang, Jian Zheng, Xing Lu, Fan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/fadcd1063a844cb497280ca7cb813575
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spelling	oai:doaj.org-article:fadcd1063a844cb497280ca7cb8135752021-11-29T00:55:53ZmiR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma2314-614110.1155/2021/1442093https://doaj.org/article/fadcd1063a844cb497280ca7cb8135752021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1442093https://doaj.org/toc/2314-6141Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay. Results. The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results. Conclusions. The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.Lihui YaoWenjing ZhangJian ZhengXing LuFan ZhangHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Lihui Yao Wenjing Zhang Jian Zheng Xing Lu Fan Zhang miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
description	Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay. Results. The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results. Conclusions. The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.
format	article
author	Lihui Yao Wenjing Zhang Jian Zheng Xing Lu Fan Zhang
author_facet	Lihui Yao Wenjing Zhang Jian Zheng Xing Lu Fan Zhang
author_sort	Lihui Yao
title	miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
title_short	miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
title_full	miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
title_fullStr	miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
title_full_unstemmed	miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma
title_sort	mir-199a targeting pnrc1 to promote keratinocyte proliferation and invasion in cholesteatoma
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/fadcd1063a844cb497280ca7cb813575
work_keys_str_mv	AT lihuiyao mir199atargetingpnrc1topromotekeratinocyteproliferationandinvasionincholesteatoma AT wenjingzhang mir199atargetingpnrc1topromotekeratinocyteproliferationandinvasionincholesteatoma AT jianzheng mir199atargetingpnrc1topromotekeratinocyteproliferationandinvasionincholesteatoma AT xinglu mir199atargetingpnrc1topromotekeratinocyteproliferationandinvasionincholesteatoma AT fanzhang mir199atargetingpnrc1topromotekeratinocyteproliferationandinvasionincholesteatoma
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miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

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