Tear protein analysis in presumed congenital alacrima

Shigeharu Yaginuma,1 Yoko Akune,2 Chika Shigeyasu,1,2 Yoji Takano,3 Masakazu Yamada1 1Department of Ophthalmology, Kyorin University School of Medicine, Tokyo 181-8611, Japan; 2Division for Vision Research, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, T...

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Autores principales:	Yaginuma S, Akune Y, Shigeyasu C, Takano Y, Yamada M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	alacrima albumin dry eye lactoferrin tears Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/fae3917499ef41c6b75642c8de623fa7
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spelling	oai:doaj.org-article:fae3917499ef41c6b75642c8de623fa72021-12-02T07:11:56ZTear protein analysis in presumed congenital alacrima1177-5483https://doaj.org/article/fae3917499ef41c6b75642c8de623fa72018-12-01T00:00:00Zhttps://www.dovepress.com/tear-protein-analysis-in-presumed-congenital-alacrima-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Shigeharu Yaginuma,1 Yoko Akune,2 Chika Shigeyasu,1,2 Yoji Takano,3 Masakazu Yamada1 1Department of Ophthalmology, Kyorin University School of Medicine, Tokyo 181-8611, Japan; 2Division for Vision Research, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo 152-8902, Japan; 3Department of Ophthalmology, Kawasaki Municipal Ida Hospital, Kawasaki 211-0035, Japan Objectives: It is often hard to reach a definitive diagnosis of congenital alacrima because of the difficultly in proving the lack of lacrimal tissue. We report here the distinct tear protein profile in presumed congenital alacrima. Patients and methods: A 13-year-old girl with presumed congenital alacrima and 15 healthy volunteers aged 23–35 years were included in this study. Tears were collected using Schirmer strips, and tear protein analyses were performed. Tear samples were collected from the patient with alacrima before and after surgical punctal occlusion of both the upper and lower puncta, and tear protein extract was fractionated using HPLC. Lactoferrin and albumin concentrations in the tears were separately determined using ELISA. Results: The chromatogram in the patient greatly differed from that in normal subjects with low concentrations of secretory IgA, lipocalin-1, and lysozyme. ELISA results indicated that the tear fluid in the patient contained a high concentration of albumin but a minimal concentration of lactoferrin. Punctal occlusion improved the ocular surface findings, but major lacrimal protein concentrations remained low and tear albumin concentrations further increased. Conclusion: The tear protein profile of a patient with presumed congenital alacrima greatly differed from that of normal subjects. Tear protein analysis may be useful in making a diagnosis of congenital alacrima and in assessing the efficacy and mechanism of punctal occlusion. Keywords: alacrima, albumin, dry eye, lactoferrin, tearsYaginuma SAkune YShigeyasu CTakano YYamada MDove Medical Pressarticlealacrimaalbumindry eyelactoferrintearsOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 2591-2595 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	alacrima albumin dry eye lactoferrin tears Ophthalmology RE1-994
spellingShingle	alacrima albumin dry eye lactoferrin tears Ophthalmology RE1-994 Yaginuma S Akune Y Shigeyasu C Takano Y Yamada M Tear protein analysis in presumed congenital alacrima
description	Shigeharu Yaginuma,1 Yoko Akune,2 Chika Shigeyasu,1,2 Yoji Takano,3 Masakazu Yamada1 1Department of Ophthalmology, Kyorin University School of Medicine, Tokyo 181-8611, Japan; 2Division for Vision Research, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo 152-8902, Japan; 3Department of Ophthalmology, Kawasaki Municipal Ida Hospital, Kawasaki 211-0035, Japan Objectives: It is often hard to reach a definitive diagnosis of congenital alacrima because of the difficultly in proving the lack of lacrimal tissue. We report here the distinct tear protein profile in presumed congenital alacrima. Patients and methods: A 13-year-old girl with presumed congenital alacrima and 15 healthy volunteers aged 23–35 years were included in this study. Tears were collected using Schirmer strips, and tear protein analyses were performed. Tear samples were collected from the patient with alacrima before and after surgical punctal occlusion of both the upper and lower puncta, and tear protein extract was fractionated using HPLC. Lactoferrin and albumin concentrations in the tears were separately determined using ELISA. Results: The chromatogram in the patient greatly differed from that in normal subjects with low concentrations of secretory IgA, lipocalin-1, and lysozyme. ELISA results indicated that the tear fluid in the patient contained a high concentration of albumin but a minimal concentration of lactoferrin. Punctal occlusion improved the ocular surface findings, but major lacrimal protein concentrations remained low and tear albumin concentrations further increased. Conclusion: The tear protein profile of a patient with presumed congenital alacrima greatly differed from that of normal subjects. Tear protein analysis may be useful in making a diagnosis of congenital alacrima and in assessing the efficacy and mechanism of punctal occlusion. Keywords: alacrima, albumin, dry eye, lactoferrin, tears
format	article
author	Yaginuma S Akune Y Shigeyasu C Takano Y Yamada M
author_facet	Yaginuma S Akune Y Shigeyasu C Takano Y Yamada M
author_sort	Yaginuma S
title	Tear protein analysis in presumed congenital alacrima
title_short	Tear protein analysis in presumed congenital alacrima
title_full	Tear protein analysis in presumed congenital alacrima
title_fullStr	Tear protein analysis in presumed congenital alacrima
title_full_unstemmed	Tear protein analysis in presumed congenital alacrima
title_sort	tear protein analysis in presumed congenital alacrima
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/fae3917499ef41c6b75642c8de623fa7
work_keys_str_mv	AT yaginumas tearproteinanalysisinpresumedcongenitalalacrima AT akuney tearproteinanalysisinpresumedcongenitalalacrima AT shigeyasuc tearproteinanalysisinpresumedcongenitalalacrima AT takanoy tearproteinanalysisinpresumedcongenitalalacrima AT yamadam tearproteinanalysisinpresumedcongenitalalacrima
_version_	1718399612313665536

Tear protein analysis in presumed congenital alacrima

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