Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers

Coleus forskohlii Briq. is an indigenous medicinal plant with high traditional use in India. Genetic analysis of 37 diverse C. forskohlii genotypes was performed using 25 RAPD primers, which yielded 117 bands, of which 60 (51.28%) were polymorphic providing an average of 3.75 bands per primer. There...

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Autores principales:	C Kavitha, E Vadivel, R Sivasamy, K Rajamani
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2009
Materias:	coleus forskohlii genetic diversity rapd Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/faeec99f8f684e4e8631657147e9d9fb
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spelling	oai:doaj.org-article:faeec99f8f684e4e8631657147e9d9fb2021-12-02T12:06:39ZAnalyzing Variability in Coleus forskohlii Briq. Using RAPD Markers0973-354X2582-4899https://doaj.org/article/faeec99f8f684e4e8631657147e9d9fb2009-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/532https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Coleus forskohlii Briq. is an indigenous medicinal plant with high traditional use in India. Genetic analysis of 37 diverse C. forskohlii genotypes was performed using 25 RAPD primers, which yielded 117 bands, of which 60 (51.28%) were polymorphic providing an average of 3.75 bands per primer. There were no genotype-specific products. The number of bands per primer varied from 1 (OPZ 8&16) to 7 (OPZ 11). Similarity matrix was constructed using Jaccard's Coefficient and the data matrix of coefficient of similarity was subjected to cluster analysis using unweighted pair group methodology with arithmetic average (UPGMA). Cluster analysis resulted in grouping of 37 genotypes into two major clusters. The results indicated that RAPD could be used for genetic diversity analysis in C. forskohlii using higher number of primers as it is reliable, easy, rapid and cost-effective.C KavithaE VadivelR SivasamyK RajamaniSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlecoleus forskohliigenetic diversityrapdPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 4, Iss 2, Pp 143-147 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	coleus forskohlii genetic diversity rapd Plant culture SB1-1110
spellingShingle	coleus forskohlii genetic diversity rapd Plant culture SB1-1110 C Kavitha E Vadivel R Sivasamy K Rajamani Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
description	Coleus forskohlii Briq. is an indigenous medicinal plant with high traditional use in India. Genetic analysis of 37 diverse C. forskohlii genotypes was performed using 25 RAPD primers, which yielded 117 bands, of which 60 (51.28%) were polymorphic providing an average of 3.75 bands per primer. There were no genotype-specific products. The number of bands per primer varied from 1 (OPZ 8&16) to 7 (OPZ 11). Similarity matrix was constructed using Jaccard's Coefficient and the data matrix of coefficient of similarity was subjected to cluster analysis using unweighted pair group methodology with arithmetic average (UPGMA). Cluster analysis resulted in grouping of 37 genotypes into two major clusters. The results indicated that RAPD could be used for genetic diversity analysis in C. forskohlii using higher number of primers as it is reliable, easy, rapid and cost-effective.
format	article
author	C Kavitha E Vadivel R Sivasamy K Rajamani
author_facet	C Kavitha E Vadivel R Sivasamy K Rajamani
author_sort	C Kavitha
title	Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
title_short	Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
title_full	Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
title_fullStr	Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
title_full_unstemmed	Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers
title_sort	analyzing variability in coleus forskohlii briq. using rapd markers
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2009
url	https://doaj.org/article/faeec99f8f684e4e8631657147e9d9fb
work_keys_str_mv	AT ckavitha analyzingvariabilityincoleusforskohliibriqusingrapdmarkers AT evadivel analyzingvariabilityincoleusforskohliibriqusingrapdmarkers AT rsivasamy analyzingvariabilityincoleusforskohliibriqusingrapdmarkers AT krajamani analyzingvariabilityincoleusforskohliibriqusingrapdmarkers
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Analyzing Variability in Coleus forskohlii Briq. Using RAPD Markers

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