Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital

Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configu...

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Autores principales:	Trupti Bajpai, M Pandey, M Varma, G S Bhatambare
Formato:	article
Lenguaje:	EN
Publicado:	Thieme Medical and Scientific Publishers Pvt. Ltd. 2017
Materias:	beta-lactam antibiotics extended-spectrum beta-lactamases polymerase chain reaction Medicine R
Acceso en línea:	https://doaj.org/article/faf43993c17b4a72aa242abf150dd6f6
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spelling	oai:doaj.org-article:faf43993c17b4a72aa242abf150dd6f62021-12-02T16:37:41ZPrevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital2231-07702249-446410.4103/2231-0770.197508https://doaj.org/article/faf43993c17b4a72aa242abf150dd6f62017-01-01T00:00:00Zhttp://www.thieme-connect.de/DOI/DOI?10.4103/2231-0770.197508https://doaj.org/toc/2231-0770https://doaj.org/toc/2249-4464Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.Trupti BajpaiM PandeyM VarmaG S BhatambareThieme Medical and Scientific Publishers Pvt. Ltd.articlebeta-lactam antibioticsextended-spectrum beta-lactamasespolymerase chain reactionMedicineRENAvicenna Journal of Medicine, Vol 07, Iss 01, Pp 12-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	beta-lactam antibiotics extended-spectrum beta-lactamases polymerase chain reaction Medicine R
spellingShingle	beta-lactam antibiotics extended-spectrum beta-lactamases polymerase chain reaction Medicine R Trupti Bajpai M Pandey M Varma G S Bhatambare Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
description	Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.
format	article
author	Trupti Bajpai M Pandey M Varma G S Bhatambare
author_facet	Trupti Bajpai M Pandey M Varma G S Bhatambare
author_sort	Trupti Bajpai
title	Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
title_short	Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
title_full	Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
title_fullStr	Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
title_full_unstemmed	Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital
title_sort	prevalence of tem, shv, and ctx-m beta-lactamase genes in the urinary isolates of a tertiary care hospital
publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
publishDate	2017
url	https://doaj.org/article/faf43993c17b4a72aa242abf150dd6f6
work_keys_str_mv	AT truptibajpai prevalenceoftemshvandctxmbetalactamasegenesintheurinaryisolatesofatertiarycarehospital AT mpandey prevalenceoftemshvandctxmbetalactamasegenesintheurinaryisolatesofatertiarycarehospital AT mvarma prevalenceoftemshvandctxmbetalactamasegenesintheurinaryisolatesofatertiarycarehospital AT gsbhatambare prevalenceoftemshvandctxmbetalactamasegenesintheurinaryisolatesofatertiarycarehospital
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Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital

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