Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands

Abstract Spider silk synthesis is an emerging model for the evolution of tissue-specific gene expression and the role of gene duplication in functional novelty, but its potential has not been fully realized. Accordingly, we quantified transcript (mRNA) abundance in seven silk gland types and three n...

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Autores principales:	Thomas H. Clarke, Jessica E. Garb, Robert A. Haney, R. Crystal Chaw, Cheryl Y. Hayashi, Nadia A. Ayoub
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fafee9fd6f4c49f9851092ef3caec1ed
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spelling	oai:doaj.org-article:fafee9fd6f4c49f9851092ef3caec1ed2021-12-02T16:08:08ZEvolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands10.1038/s41598-017-07388-12045-2322https://doaj.org/article/fafee9fd6f4c49f9851092ef3caec1ed2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07388-1https://doaj.org/toc/2045-2322Abstract Spider silk synthesis is an emerging model for the evolution of tissue-specific gene expression and the role of gene duplication in functional novelty, but its potential has not been fully realized. Accordingly, we quantified transcript (mRNA) abundance in seven silk gland types and three non-silk gland tissues for three cobweb-weaving spider species. Evolutionary analyses based on expression levels of thousands of homologous transcripts and phylogenetic reconstruction of 605 gene families demonstrated conservation of expression for each gland type among species. Despite serial homology of all silk glands, the expression profiles of the glue-forming aggregate glands were divergent from fiber-forming glands. Also surprising was our finding that shifts in gene expression among silk gland types were not necessarily coupled with gene duplication, even though silk-specific genes belong to multi-paralog gene families. Our results challenge widely accepted models of tissue specialization and significantly advance efforts to replicate silk-based high-performance biomaterials.Thomas H. ClarkeJessica E. GarbRobert A. HaneyR. Crystal ChawCheryl Y. HayashiNadia A. AyoubNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas H. Clarke Jessica E. Garb Robert A. Haney R. Crystal Chaw Cheryl Y. Hayashi Nadia A. Ayoub Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
description	Abstract Spider silk synthesis is an emerging model for the evolution of tissue-specific gene expression and the role of gene duplication in functional novelty, but its potential has not been fully realized. Accordingly, we quantified transcript (mRNA) abundance in seven silk gland types and three non-silk gland tissues for three cobweb-weaving spider species. Evolutionary analyses based on expression levels of thousands of homologous transcripts and phylogenetic reconstruction of 605 gene families demonstrated conservation of expression for each gland type among species. Despite serial homology of all silk glands, the expression profiles of the glue-forming aggregate glands were divergent from fiber-forming glands. Also surprising was our finding that shifts in gene expression among silk gland types were not necessarily coupled with gene duplication, even though silk-specific genes belong to multi-paralog gene families. Our results challenge widely accepted models of tissue specialization and significantly advance efforts to replicate silk-based high-performance biomaterials.
format	article
author	Thomas H. Clarke Jessica E. Garb Robert A. Haney R. Crystal Chaw Cheryl Y. Hayashi Nadia A. Ayoub
author_facet	Thomas H. Clarke Jessica E. Garb Robert A. Haney R. Crystal Chaw Cheryl Y. Hayashi Nadia A. Ayoub
author_sort	Thomas H. Clarke
title	Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
title_short	Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
title_full	Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
title_fullStr	Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
title_full_unstemmed	Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
title_sort	evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fafee9fd6f4c49f9851092ef3caec1ed
work_keys_str_mv	AT thomashclarke evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands AT jessicaegarb evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands AT robertahaney evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands AT rcrystalchaw evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands AT cherylyhayashi evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands AT nadiaaayoub evolutionaryshiftsingeneexpressiondecoupledfromgeneduplicationacrossfunctionallydistinctspidersilkglands
_version_	1718384633963347968

Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands

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