Studying Neutrophil Function in vitro: Cell Models and Environmental Factors

Marfa Blanter, Mieke Gouwy, Sofie Struyf Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven 3000, BelgiumCorrespondence: Sofie Struyf Rega Institute - Herestraat 49 – Bus 1042, Le...

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Autores principales:	Blanter M, Gouwy M, Struyf S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	neutrophils hl-60 plb-985 nb4 kasumi-1 induced pluripotent stem cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/faffab34a23940b08cd7a936c50717cd
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spelling	oai:doaj.org-article:faffab34a23940b08cd7a936c50717cd2021-12-02T10:56:37ZStudying Neutrophil Function in vitro: Cell Models and Environmental Factors1178-7031https://doaj.org/article/faffab34a23940b08cd7a936c50717cd2021-01-01T00:00:00Zhttps://www.dovepress.com/studying-neutrophil-function-in-vitro-cell-models-and-environmental-fa-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Marfa Blanter, Mieke Gouwy, Sofie Struyf Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven 3000, BelgiumCorrespondence: Sofie Struyf Rega Institute - Herestraat 49 – Bus 1042, Leuven 3000, BelgiumTel +32 16 32 24 22Email sofie.struyf@kuleuven.beAbstract: Neutrophils are the most abundant immune cell type in the blood and constitute the first line of defense against invading pathogens. Despite their important role in many diseases, they are challenging to study due to their short life span and the inability to cryopreserve or expand them in vitro. Thus, research into neutrophils has to rely on cells freshly isolated from peripheral blood of human donors, introducing donor-dependent variation in the experimental data. To counteract these problems, researchers tried to develop adequate cell models, such as cell lines. For those functional studies that cannot rely on cell models, a standardization of protocols regarding neutrophil purification and culturing could be a solution. In this review, we provide an overview of the most commonly used models for neutrophil function (HL-60, PLB-985, NB4, Kasumi-1 and induced pluripotent stem cells). In addition, we describe the effects of glucose concentration, pH, oxygen tension and temperature on neutrophil function.Keywords: neutrophils, HL-60, PLB-985, NB4, Kasumi-1, induced pluripotent stem cellsBlanter MGouwy MStruyf SDove Medical Pressarticleneutrophilshl-60plb-985nb4kasumi-1induced pluripotent stem cellsPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 141-162 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neutrophils hl-60 plb-985 nb4 kasumi-1 induced pluripotent stem cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	neutrophils hl-60 plb-985 nb4 kasumi-1 induced pluripotent stem cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Blanter M Gouwy M Struyf S Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
description	Marfa Blanter, Mieke Gouwy, Sofie Struyf Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven 3000, BelgiumCorrespondence: Sofie Struyf Rega Institute - Herestraat 49 – Bus 1042, Leuven 3000, BelgiumTel +32 16 32 24 22Email sofie.struyf@kuleuven.beAbstract: Neutrophils are the most abundant immune cell type in the blood and constitute the first line of defense against invading pathogens. Despite their important role in many diseases, they are challenging to study due to their short life span and the inability to cryopreserve or expand them in vitro. Thus, research into neutrophils has to rely on cells freshly isolated from peripheral blood of human donors, introducing donor-dependent variation in the experimental data. To counteract these problems, researchers tried to develop adequate cell models, such as cell lines. For those functional studies that cannot rely on cell models, a standardization of protocols regarding neutrophil purification and culturing could be a solution. In this review, we provide an overview of the most commonly used models for neutrophil function (HL-60, PLB-985, NB4, Kasumi-1 and induced pluripotent stem cells). In addition, we describe the effects of glucose concentration, pH, oxygen tension and temperature on neutrophil function.Keywords: neutrophils, HL-60, PLB-985, NB4, Kasumi-1, induced pluripotent stem cells
format	article
author	Blanter M Gouwy M Struyf S
author_facet	Blanter M Gouwy M Struyf S
author_sort	Blanter M
title	Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
title_short	Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
title_full	Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
title_fullStr	Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
title_full_unstemmed	Studying Neutrophil Function in vitro: Cell Models and Environmental Factors
title_sort	studying neutrophil function in vitro: cell models and environmental factors
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/faffab34a23940b08cd7a936c50717cd
work_keys_str_mv	AT blanterm studyingneutrophilfunctioninvitrocellmodelsandenvironmentalfactors AT gouwym studyingneutrophilfunctioninvitrocellmodelsandenvironmentalfactors AT struyfs studyingneutrophilfunctioninvitrocellmodelsandenvironmentalfactors
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Studying Neutrophil Function in vitro: Cell Models and Environmental Factors

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