Traumatic brain injury results in acute rarefication of the vascular network

Abstract The role of the cerebrovascular network and its acute response to TBI is poorly defined and emerging evidence suggests that cerebrovascular reactivity is altered. We explored how cortical vessels are physically altered following TBI using a newly developed technique, vessel painting. We tes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andre Obenaus, Michelle Ng, Amanda M. Orantes, Eli Kinney-Lang, Faisal Rashid, Mary Hamer, Richard A. DeFazio, Jiping Tang, John H. Zhang, William J. Pearce
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb10b248f09a471caf096a3e5d92ed51
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb10b248f09a471caf096a3e5d92ed51
record_format	dspace
spelling	oai:doaj.org-article:fb10b248f09a471caf096a3e5d92ed512021-12-02T12:32:57ZTraumatic brain injury results in acute rarefication of the vascular network10.1038/s41598-017-00161-42045-2322https://doaj.org/article/fb10b248f09a471caf096a3e5d92ed512017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00161-4https://doaj.org/toc/2045-2322Abstract The role of the cerebrovascular network and its acute response to TBI is poorly defined and emerging evidence suggests that cerebrovascular reactivity is altered. We explored how cortical vessels are physically altered following TBI using a newly developed technique, vessel painting. We tested our hypothesis that a focal moderate TBI results in global decrements to structural aspects of the vasculature. Rats (naïve, sham-operated, TBI) underwent a moderate controlled cortical impact. Animals underwent vessel painting perfusion to label the entire cortex at 1 day post TBI followed by whole brain axial and coronal images using a wide-field fluorescence microscope. Cortical vessel network characteristics were analyzed for classical angiographic features (junctions, lengths) wherein we observed significant global (both hemispheres) reductions in vessel junctions and vessel lengths of 33% and 22%, respectively. Biological complexity can be quantified using fractal geometric features where we observed that fractal measures were also reduced significantly by 33%, 16% and 13% for kurtosis, peak value frequency and skewness, respectively. Acutely after TBI there is a reduction in vascular network and vascular complexity that are exacerbated at the lesion site and provide structural evidence for the bilateral hemodynamic alterations that have been reported in patients after TBI.Andre ObenausMichelle NgAmanda M. OrantesEli Kinney-LangFaisal RashidMary HamerRichard A. DeFazioJiping TangJohn H. ZhangWilliam J. PearceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andre Obenaus Michelle Ng Amanda M. Orantes Eli Kinney-Lang Faisal Rashid Mary Hamer Richard A. DeFazio Jiping Tang John H. Zhang William J. Pearce Traumatic brain injury results in acute rarefication of the vascular network
description	Abstract The role of the cerebrovascular network and its acute response to TBI is poorly defined and emerging evidence suggests that cerebrovascular reactivity is altered. We explored how cortical vessels are physically altered following TBI using a newly developed technique, vessel painting. We tested our hypothesis that a focal moderate TBI results in global decrements to structural aspects of the vasculature. Rats (naïve, sham-operated, TBI) underwent a moderate controlled cortical impact. Animals underwent vessel painting perfusion to label the entire cortex at 1 day post TBI followed by whole brain axial and coronal images using a wide-field fluorescence microscope. Cortical vessel network characteristics were analyzed for classical angiographic features (junctions, lengths) wherein we observed significant global (both hemispheres) reductions in vessel junctions and vessel lengths of 33% and 22%, respectively. Biological complexity can be quantified using fractal geometric features where we observed that fractal measures were also reduced significantly by 33%, 16% and 13% for kurtosis, peak value frequency and skewness, respectively. Acutely after TBI there is a reduction in vascular network and vascular complexity that are exacerbated at the lesion site and provide structural evidence for the bilateral hemodynamic alterations that have been reported in patients after TBI.
format	article
author	Andre Obenaus Michelle Ng Amanda M. Orantes Eli Kinney-Lang Faisal Rashid Mary Hamer Richard A. DeFazio Jiping Tang John H. Zhang William J. Pearce
author_facet	Andre Obenaus Michelle Ng Amanda M. Orantes Eli Kinney-Lang Faisal Rashid Mary Hamer Richard A. DeFazio Jiping Tang John H. Zhang William J. Pearce
author_sort	Andre Obenaus
title	Traumatic brain injury results in acute rarefication of the vascular network
title_short	Traumatic brain injury results in acute rarefication of the vascular network
title_full	Traumatic brain injury results in acute rarefication of the vascular network
title_fullStr	Traumatic brain injury results in acute rarefication of the vascular network
title_full_unstemmed	Traumatic brain injury results in acute rarefication of the vascular network
title_sort	traumatic brain injury results in acute rarefication of the vascular network
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fb10b248f09a471caf096a3e5d92ed51
work_keys_str_mv	AT andreobenaus traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT michelleng traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT amandamorantes traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT elikinneylang traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT faisalrashid traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT maryhamer traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT richardadefazio traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT jipingtang traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT johnhzhang traumaticbraininjuryresultsinacuterareficationofthevascularnetwork AT williamjpearce traumaticbraininjuryresultsinacuterareficationofthevascularnetwork
_version_	1718393956401676288

Traumatic brain injury results in acute rarefication of the vascular network

Ejemplares similares