Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification

BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopatho­...

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Autores principales: Sara Moreno, Oscar Maiques, Carla Barcelo, Marta Romero, Maria Santacana, Ignacio Gómez, Dolors Cuevas, Ana Velasco, Alvar Vea, Anna Macia, Ramon Boix, Joan Valls, Sonia Gatius, Carles Canti, Xavier Matias-Guiu, Xavier Soria, Rosa M. Marti
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Publicado: Society for Publication of Acta Dermato-Venereologica 2021
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spelling oai:doaj.org-article:fb10f1cd9c824c5b98cd290cac95a8502021-11-24T13:54:07ZDifferential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification10.2340/actadv.v101.3610001-55551651-2057https://doaj.org/article/fb10f1cd9c824c5b98cd290cac95a8502021-11-01T00:00:00Zhttps://medicaljournalssweden.se/actadv/article/view/361https://doaj.org/toc/0001-5555https://doaj.org/toc/1651-2057BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopatho­logically and dermoscopically classified acquired naevi. Regarding BRAF/V600E, dysplastic naevi showed lower immunostaining than common naevi, which was significant in comparison with intradermal naevi, which showed the highest BRAF/V600E histoscore. Junctional naevi showed the lowest BRAF/V600E levels. Globular/cobblestone and reticular dermoscopic patterns were consistently associated with high and low BRAF/V600E immunoexpression, respectively, but Zalaudek’s peripheral globule pattern (CR/PG) showed the highest BRAF/V600E immunoexpression. Among global patterns, the previously not investigated multicomponent pattern showed the lowest BRAF/V600E immunoexpression. Regarding the remaining biomarkers, new immunohistochemical features were found, in particular p16 and PTEN low expression in multicomponent pattern; and Ki67, hTERT and Cav.3.1 high expression in CR/PG. In conclusion, histopathology and dermoscopy provide complementary information regarding the biology of melanocytic naevi. Sara MorenoOscar MaiquesCarla BarceloMarta RomeroMaria SantacanaIgnacio GómezDolors CuevasAna VelascoAlvar VeaAnna MaciaRamon BoixJoan VallsSonia GatiusCarles CantiXavier Matias-GuiuXavier SoriaRosa M. MartiSociety for Publication of Acta Dermato-Venereologicaarticleacquired melanocytic naevusBRAF-V600Esenescence markersPTENT-type calcium channelsDermatologyRL1-803ENActa Dermato-Venereologica, Vol 101, Iss 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic acquired melanocytic naevus
BRAF-V600E
senescence markers
PTEN
T-type calcium channels
Dermatology
RL1-803
spellingShingle acquired melanocytic naevus
BRAF-V600E
senescence markers
PTEN
T-type calcium channels
Dermatology
RL1-803
Sara Moreno
Oscar Maiques
Carla Barcelo
Marta Romero
Maria Santacana
Ignacio Gómez
Dolors Cuevas
Ana Velasco
Alvar Vea
Anna Macia
Ramon Boix
Joan Valls
Sonia Gatius
Carles Canti
Xavier Matias-Guiu
Xavier Soria
Rosa M. Marti
Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
description BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopatho­logically and dermoscopically classified acquired naevi. Regarding BRAF/V600E, dysplastic naevi showed lower immunostaining than common naevi, which was significant in comparison with intradermal naevi, which showed the highest BRAF/V600E histoscore. Junctional naevi showed the lowest BRAF/V600E levels. Globular/cobblestone and reticular dermoscopic patterns were consistently associated with high and low BRAF/V600E immunoexpression, respectively, but Zalaudek’s peripheral globule pattern (CR/PG) showed the highest BRAF/V600E immunoexpression. Among global patterns, the previously not investigated multicomponent pattern showed the lowest BRAF/V600E immunoexpression. Regarding the remaining biomarkers, new immunohistochemical features were found, in particular p16 and PTEN low expression in multicomponent pattern; and Ki67, hTERT and Cav.3.1 high expression in CR/PG. In conclusion, histopathology and dermoscopy provide complementary information regarding the biology of melanocytic naevi.
format article
author Sara Moreno
Oscar Maiques
Carla Barcelo
Marta Romero
Maria Santacana
Ignacio Gómez
Dolors Cuevas
Ana Velasco
Alvar Vea
Anna Macia
Ramon Boix
Joan Valls
Sonia Gatius
Carles Canti
Xavier Matias-Guiu
Xavier Soria
Rosa M. Marti
author_facet Sara Moreno
Oscar Maiques
Carla Barcelo
Marta Romero
Maria Santacana
Ignacio Gómez
Dolors Cuevas
Ana Velasco
Alvar Vea
Anna Macia
Ramon Boix
Joan Valls
Sonia Gatius
Carles Canti
Xavier Matias-Guiu
Xavier Soria
Rosa M. Marti
author_sort Sara Moreno
title Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
title_short Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
title_full Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
title_fullStr Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
title_full_unstemmed Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
title_sort differential immunoexpression of braf/v600e, senescence markers, pten, and t-type calcium channels in acquired naevi according to their histopathological and dermoscopic classification
publisher Society for Publication of Acta Dermato-Venereologica
publishDate 2021
url https://doaj.org/article/fb10f1cd9c824c5b98cd290cac95a850
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