LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis

Abstract Purpose Ovarian cancer (OC) is the most common malignancy in women with high mortality. Increasing studies have revealed that long non-coding RNA (lncRNA) MNX1-AS1 has a promoting effect on various cancers. However, the mechanisms of MNX1-AS1 in OC are still unclear. Therefore, this study f...

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Autores principales:	Yang Shen, Mengmeng Lv, Yichen Fang, Jin Lu, Yuzhong Wu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Ovarian cancer Long non-coding RNA MNX1-AS1 MiR-744-5p SOX12 Gynecology and obstetrics RG1-991
Acceso en línea:	https://doaj.org/article/fb14f69f1d074935bdd292f964d7aed3
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spelling	oai:doaj.org-article:fb14f69f1d074935bdd292f964d7aed32021-11-21T12:32:13ZLncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis10.1186/s13048-021-00910-01757-2215https://doaj.org/article/fb14f69f1d074935bdd292f964d7aed32021-11-01T00:00:00Zhttps://doi.org/10.1186/s13048-021-00910-0https://doaj.org/toc/1757-2215Abstract Purpose Ovarian cancer (OC) is the most common malignancy in women with high mortality. Increasing studies have revealed that long non-coding RNA (lncRNA) MNX1-AS1 has a promoting effect on various cancers. However, the mechanisms of MNX1-AS1 in OC are still unclear. Therefore, this study focused on exploring the mechanisms of MNX1-AS1 in OC. Materials and methods The expression of SOX12 at the protein level was detected by western blot. Cell proliferation was detected by CCK8 assay and colony formation assay. Cell cycle and cell apoptosis were detected by flow cytometry. Wound-healing assay, transwell assay and western blot were used to detect the ability of cell migration and invasion. The target binding was confirmed through the luciferase reporter assay. Results The expression of MNX1-AS1 was increased in OC tumor tissues and cells. Elevated MNX1-AS1 expression is associated with advanced stage and lower overall survival rate. Knockdown of MNX1-AS1 inhibited cell proliferation, migration and invasion, blocked cell cycle, and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells. MNX1-AS1 was competitively binding with miR-744-5p, and its downstream target gene was SOX12. miR-544-5p expression was decreased, while SOX12 expression was increased in OC tumor tissues and cells. Overexpression of miR-744-5p inhibited cell proliferation, migration, invasion and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells. Conclusion MNX1-AS1 promoted the development of OC through miR-744-5p/SOX12 axis. This study revealed a novel mechanism of MNX1-AS1 in OC, which may provide a new treatment or scanning target for OC.Yang ShenMengmeng LvYichen FangJin LuYuzhong WuBMCarticleOvarian cancerLong non-coding RNAMNX1-AS1MiR-744-5pSOX12Gynecology and obstetricsRG1-991ENJournal of Ovarian Research, Vol 14, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ovarian cancer Long non-coding RNA MNX1-AS1 MiR-744-5p SOX12 Gynecology and obstetrics RG1-991
spellingShingle	Ovarian cancer Long non-coding RNA MNX1-AS1 MiR-744-5p SOX12 Gynecology and obstetrics RG1-991 Yang Shen Mengmeng Lv Yichen Fang Jin Lu Yuzhong Wu LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
description	Abstract Purpose Ovarian cancer (OC) is the most common malignancy in women with high mortality. Increasing studies have revealed that long non-coding RNA (lncRNA) MNX1-AS1 has a promoting effect on various cancers. However, the mechanisms of MNX1-AS1 in OC are still unclear. Therefore, this study focused on exploring the mechanisms of MNX1-AS1 in OC. Materials and methods The expression of SOX12 at the protein level was detected by western blot. Cell proliferation was detected by CCK8 assay and colony formation assay. Cell cycle and cell apoptosis were detected by flow cytometry. Wound-healing assay, transwell assay and western blot were used to detect the ability of cell migration and invasion. The target binding was confirmed through the luciferase reporter assay. Results The expression of MNX1-AS1 was increased in OC tumor tissues and cells. Elevated MNX1-AS1 expression is associated with advanced stage and lower overall survival rate. Knockdown of MNX1-AS1 inhibited cell proliferation, migration and invasion, blocked cell cycle, and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells. MNX1-AS1 was competitively binding with miR-744-5p, and its downstream target gene was SOX12. miR-544-5p expression was decreased, while SOX12 expression was increased in OC tumor tissues and cells. Overexpression of miR-744-5p inhibited cell proliferation, migration, invasion and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells. Conclusion MNX1-AS1 promoted the development of OC through miR-744-5p/SOX12 axis. This study revealed a novel mechanism of MNX1-AS1 in OC, which may provide a new treatment or scanning target for OC.
format	article
author	Yang Shen Mengmeng Lv Yichen Fang Jin Lu Yuzhong Wu
author_facet	Yang Shen Mengmeng Lv Yichen Fang Jin Lu Yuzhong Wu
author_sort	Yang Shen
title	LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
title_short	LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
title_full	LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
title_fullStr	LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
title_full_unstemmed	LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis
title_sort	lncrna mnx1-as1 promotes ovarian cancer process via targeting the mir-744-5p/sox12 axis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/fb14f69f1d074935bdd292f964d7aed3
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LncRNA MNX1-AS1 promotes ovarian cancer process via targeting the miR-744-5p/SOX12 axis

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