The complex exogenous RNA spectra in human plasma: an interface with human gut biota?

Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we o...

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Autores principales:	Kai Wang, Hong Li, Yue Yuan, Alton Etheridge, Yong Zhou, David Huang, Paul Wilmes, David Galas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb1acdd82e79475eb9af28ac36c01147
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spelling	oai:doaj.org-article:fb1acdd82e79475eb9af28ac36c011472021-11-18T08:05:50ZThe complex exogenous RNA spectra in human plasma: an interface with human gut biota?1932-620310.1371/journal.pone.0051009https://doaj.org/article/fb1acdd82e79475eb9af28ac36c011472012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251414/?tool=EBIhttps://doaj.org/toc/1932-6203Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we observed that a significant fraction of the circulating RNA appear to originate from exogenous species. With careful analysis of sequence error statistics and other controls, we demonstrated that there is a wide range of RNA from many different organisms, including bacteria and fungi as well as from other species. These RNAs may be associated with protein, lipid or other molecules protecting them from RNase activity in plasma. Some of these RNAs are detected in intracellular complexes and may be able to influence cellular activities under in vitro conditions. These findings raise the possibility that plasma RNAs of exogenous origin may serve as signaling molecules mediating for example the human-microbiome interaction and may affect and/or indicate the state of human health.Kai WangHong LiYue YuanAlton EtheridgeYong ZhouDavid HuangPaul WilmesDavid GalasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51009 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Wang Hong Li Yue Yuan Alton Etheridge Yong Zhou David Huang Paul Wilmes David Galas The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
description	Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we observed that a significant fraction of the circulating RNA appear to originate from exogenous species. With careful analysis of sequence error statistics and other controls, we demonstrated that there is a wide range of RNA from many different organisms, including bacteria and fungi as well as from other species. These RNAs may be associated with protein, lipid or other molecules protecting them from RNase activity in plasma. Some of these RNAs are detected in intracellular complexes and may be able to influence cellular activities under in vitro conditions. These findings raise the possibility that plasma RNAs of exogenous origin may serve as signaling molecules mediating for example the human-microbiome interaction and may affect and/or indicate the state of human health.
format	article
author	Kai Wang Hong Li Yue Yuan Alton Etheridge Yong Zhou David Huang Paul Wilmes David Galas
author_facet	Kai Wang Hong Li Yue Yuan Alton Etheridge Yong Zhou David Huang Paul Wilmes David Galas
author_sort	Kai Wang
title	The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
title_short	The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
title_full	The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
title_fullStr	The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
title_full_unstemmed	The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
title_sort	complex exogenous rna spectra in human plasma: an interface with human gut biota?
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fb1acdd82e79475eb9af28ac36c01147
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The complex exogenous RNA spectra in human plasma: an interface with human gut biota?

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