Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury

Abstract In this study, GFP-MSCs were topically applied to the surface of cerebral cortex within 1 hour of experimental TBI. No treatment was given to the control group. Three days after topical application, the MSCs homed to the injured parenchyma and improved the neurological function. Topical MSC...

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Autores principales:	Ping K. Lam, Kevin K. W. Wang, Anthony W. I. Lo, Cindy S. W. Tong, Don W. C. Ching, Kenneth Wong, Zhihui Yang, Themis Kong, Kin K. Y. Lo, Richard K. W. Choy, Paul B. S. Lai, George K. C. Wong, Wai S. Poon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb1e4c4f80be4a91b599f10cc86a1c7f
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spelling	oai:doaj.org-article:fb1e4c4f80be4a91b599f10cc86a1c7f2021-12-02T15:06:12ZInteractome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury10.1038/s41598-017-01772-72045-2322https://doaj.org/article/fb1e4c4f80be4a91b599f10cc86a1c7f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01772-7https://doaj.org/toc/2045-2322Abstract In this study, GFP-MSCs were topically applied to the surface of cerebral cortex within 1 hour of experimental TBI. No treatment was given to the control group. Three days after topical application, the MSCs homed to the injured parenchyma and improved the neurological function. Topical MSCs triggered earlier astrocytosis and reactive microglia. TBI penumbra and hippocampus had higher cellular proliferation. Apoptosis was suppressed at hippocampus at 1 week and reduced neuronal damaged was found in the penumbral at day 14 apoptosis. Proteolytic neuronal injury biomarkers (alphaII-spectrin breakdown products, SBDPs) and glial cell injury biomarker, glial fibrillary acidic protein (GFAP)-breakdown product (GBDPs) in injured cortex were also attenuated by MSCs. In the penumbra, six genes related to axongenesis (Erbb2); growth factors (Artn, Ptn); cytokine (IL3); cell cycle (Hdac4); and notch signaling (Hes1) were up-regulated three days after MSC transplant. Transcriptome analysis demonstrated that 7,943 genes were differentially expressed and 94 signaling pathways were activated in the topical MSCs transplanted onto the cortex of brain injured rats with TBI. In conclusion, topical application offers a direct and efficient delivery of MSCs to the brain.Ping K. LamKevin K. W. WangAnthony W. I. LoCindy S. W. TongDon W. C. ChingKenneth WongZhihui YangThemis KongKin K. Y. LoRichard K. W. ChoyPaul B. S. LaiGeorge K. C. WongWai S. PoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping K. Lam Kevin K. W. Wang Anthony W. I. Lo Cindy S. W. Tong Don W. C. Ching Kenneth Wong Zhihui Yang Themis Kong Kin K. Y. Lo Richard K. W. Choy Paul B. S. Lai George K. C. Wong Wai S. Poon Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
description	Abstract In this study, GFP-MSCs were topically applied to the surface of cerebral cortex within 1 hour of experimental TBI. No treatment was given to the control group. Three days after topical application, the MSCs homed to the injured parenchyma and improved the neurological function. Topical MSCs triggered earlier astrocytosis and reactive microglia. TBI penumbra and hippocampus had higher cellular proliferation. Apoptosis was suppressed at hippocampus at 1 week and reduced neuronal damaged was found in the penumbral at day 14 apoptosis. Proteolytic neuronal injury biomarkers (alphaII-spectrin breakdown products, SBDPs) and glial cell injury biomarker, glial fibrillary acidic protein (GFAP)-breakdown product (GBDPs) in injured cortex were also attenuated by MSCs. In the penumbra, six genes related to axongenesis (Erbb2); growth factors (Artn, Ptn); cytokine (IL3); cell cycle (Hdac4); and notch signaling (Hes1) were up-regulated three days after MSC transplant. Transcriptome analysis demonstrated that 7,943 genes were differentially expressed and 94 signaling pathways were activated in the topical MSCs transplanted onto the cortex of brain injured rats with TBI. In conclusion, topical application offers a direct and efficient delivery of MSCs to the brain.
format	article
author	Ping K. Lam Kevin K. W. Wang Anthony W. I. Lo Cindy S. W. Tong Don W. C. Ching Kenneth Wong Zhihui Yang Themis Kong Kin K. Y. Lo Richard K. W. Choy Paul B. S. Lai George K. C. Wong Wai S. Poon
author_facet	Ping K. Lam Kevin K. W. Wang Anthony W. I. Lo Cindy S. W. Tong Don W. C. Ching Kenneth Wong Zhihui Yang Themis Kong Kin K. Y. Lo Richard K. W. Choy Paul B. S. Lai George K. C. Wong Wai S. Poon
author_sort	Ping K. Lam
title	Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
title_short	Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
title_full	Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
title_fullStr	Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
title_full_unstemmed	Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
title_sort	interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fb1e4c4f80be4a91b599f10cc86a1c7f
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Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury

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