Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)

Mungbean yellow mosaic virus (MYMV) causes yield loss up to 80 % and is becoming problematic in French bean growing areas. Molecular marker linked selection to MYMV resistance is helpful in rapid identification of genotypes carrying resistant genes. Hence, the present study was undertaken to identif...

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Autores principales:	K V Ravishankar, T S Aghora, N Mohan, A H Naveen, M Krishnareddy
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2009
Materias:	bulk segregant analysis mymv phaseolus vulgaris l. rapd marker Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/fb2c25a1b18b4ae595e8a7f5853c88f8
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spelling	oai:doaj.org-article:fb2c25a1b18b4ae595e8a7f5853c88f82021-12-02T12:06:39ZIdentification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)0973-354X2582-4899https://doaj.org/article/fb2c25a1b18b4ae595e8a7f5853c88f82009-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/538https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Mungbean yellow mosaic virus (MYMV) causes yield loss up to 80 % and is becoming problematic in French bean growing areas. Molecular marker linked selection to MYMV resistance is helpful in rapid identification of genotypes carrying resistant genes. Hence, the present study was undertaken to identify the RAPD marker associated with MYMV resistance in French bean (Phaseolus vulgaris L.). Bulk segregant analysis (BSA) was used to identify RAPD marker linked to MYMV resistance. More than 140 random decamers were surveyed for identification of polymorphic markers between the DNA bulks of resistant and susceptible F2 individuals and their contrasting parents. Ninety eight per cent of these primers amplified DNA in both parents and bulks. Twenty two primers produced specific bands for resistant parent which was absent in the susceptible parent. Out of 22 primers, four primers produced specific fragments viz., OPG 13458, OPX 5670, OPW 17380 and OPP 07730, respectively in resistant parent and bulk, which were absent in susceptible parent and bulk. Amplification of individual DNA samples of segregating F2 resistant individuals using putative marker, OPP 07730, a decamer revealed polymorphism in all four resistant and four susceptible F2 segregants, indicating that the marker OPP 07730 was associated with MYMV resistance in IC-525260, a resistant genotype.K V RavishankarT S AghoraN MohanA H NaveenM KrishnareddySociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlebulk segregant analysismymvphaseolus vulgaris l.rapd markerPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 4, Iss 2, Pp 167-169 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	bulk segregant analysis mymv phaseolus vulgaris l. rapd marker Plant culture SB1-1110
spellingShingle	bulk segregant analysis mymv phaseolus vulgaris l. rapd marker Plant culture SB1-1110 K V Ravishankar T S Aghora N Mohan A H Naveen M Krishnareddy Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
description	Mungbean yellow mosaic virus (MYMV) causes yield loss up to 80 % and is becoming problematic in French bean growing areas. Molecular marker linked selection to MYMV resistance is helpful in rapid identification of genotypes carrying resistant genes. Hence, the present study was undertaken to identify the RAPD marker associated with MYMV resistance in French bean (Phaseolus vulgaris L.). Bulk segregant analysis (BSA) was used to identify RAPD marker linked to MYMV resistance. More than 140 random decamers were surveyed for identification of polymorphic markers between the DNA bulks of resistant and susceptible F2 individuals and their contrasting parents. Ninety eight per cent of these primers amplified DNA in both parents and bulks. Twenty two primers produced specific bands for resistant parent which was absent in the susceptible parent. Out of 22 primers, four primers produced specific fragments viz., OPG 13458, OPX 5670, OPW 17380 and OPP 07730, respectively in resistant parent and bulk, which were absent in susceptible parent and bulk. Amplification of individual DNA samples of segregating F2 resistant individuals using putative marker, OPP 07730, a decamer revealed polymorphism in all four resistant and four susceptible F2 segregants, indicating that the marker OPP 07730 was associated with MYMV resistance in IC-525260, a resistant genotype.
format	article
author	K V Ravishankar T S Aghora N Mohan A H Naveen M Krishnareddy
author_facet	K V Ravishankar T S Aghora N Mohan A H Naveen M Krishnareddy
author_sort	K V Ravishankar
title	Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
title_short	Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
title_full	Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
title_fullStr	Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
title_full_unstemmed	Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)
title_sort	identification of rapd marker linked to mungbean yellow mosaic virus resistance in french bean (phaseolus vulgaris l.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2009
url	https://doaj.org/article/fb2c25a1b18b4ae595e8a7f5853c88f8
work_keys_str_mv	AT kvravishankar identificationofrapdmarkerlinkedtomungbeanyellowmosaicvirusresistanceinfrenchbeanphaseolusvulgarisl AT tsaghora identificationofrapdmarkerlinkedtomungbeanyellowmosaicvirusresistanceinfrenchbeanphaseolusvulgarisl AT nmohan identificationofrapdmarkerlinkedtomungbeanyellowmosaicvirusresistanceinfrenchbeanphaseolusvulgarisl AT ahnaveen identificationofrapdmarkerlinkedtomungbeanyellowmosaicvirusresistanceinfrenchbeanphaseolusvulgarisl AT mkrishnareddy identificationofrapdmarkerlinkedtomungbeanyellowmosaicvirusresistanceinfrenchbeanphaseolusvulgarisl
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Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)

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