Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s...

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Autores principales:	Asghar Fallah, Mahdieh Alipour, Zahra Jamali, Akbar Farjadfar, Leila Roshangar, Minoo Partovi Nasr, Parisa Hashemi, Marziyeh Aghazadeh
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/fb379b05e0db4e6fbce6090d69de2d14
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spelling	oai:doaj.org-article:fb379b05e0db4e6fbce6090d69de2d142021-11-22T01:09:51ZOverexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells2314-614110.1155/2021/7031492https://doaj.org/article/fb379b05e0db4e6fbce6090d69de2d142021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/7031492https://doaj.org/toc/2314-6141Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s jelly-derived stem cells (WJSCs). For this purpose, human BMP2 and miR-424 DNA codes were cloned in the third generation of lentiviral vectors and then used for HEK-293T cell transfection. Lentiviral plasmids contained miR424, BMP-2, miR424-BMP2, green fluorescent protein (GFP) genes, and helper vectors. The recombinant lentiviral particles transduced the WJSCs, and the osteogenesis was evaluated by real-time PCR, Western blot, Alizarin Red staining, and alkaline phosphatase enzyme activity. According to the results, there was a significant increase in the expression of the BMP2 gene and secretion of Osteocalcin protein in the group of miR424-BMP2. Moreover, the amount of dye deposition in Alizarin Red staining and alkaline phosphatase activity was significantly higher in the mentioned group (p<0.05). Thus, the current study results clarify the efficacy of gene therapy by miR424-BMP2 vectors for bone tissue engineering. These data could help guide the development of gene therapy-based protocols for bone tissue engineering.Asghar FallahMahdieh AlipourZahra JamaliAkbar FarjadfarLeila RoshangarMinoo Partovi NasrParisa HashemiMarziyeh AghazadehHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Asghar Fallah Mahdieh Alipour Zahra Jamali Akbar Farjadfar Leila Roshangar Minoo Partovi Nasr Parisa Hashemi Marziyeh Aghazadeh Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
description	Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s jelly-derived stem cells (WJSCs). For this purpose, human BMP2 and miR-424 DNA codes were cloned in the third generation of lentiviral vectors and then used for HEK-293T cell transfection. Lentiviral plasmids contained miR424, BMP-2, miR424-BMP2, green fluorescent protein (GFP) genes, and helper vectors. The recombinant lentiviral particles transduced the WJSCs, and the osteogenesis was evaluated by real-time PCR, Western blot, Alizarin Red staining, and alkaline phosphatase enzyme activity. According to the results, there was a significant increase in the expression of the BMP2 gene and secretion of Osteocalcin protein in the group of miR424-BMP2. Moreover, the amount of dye deposition in Alizarin Red staining and alkaline phosphatase activity was significantly higher in the mentioned group (p<0.05). Thus, the current study results clarify the efficacy of gene therapy by miR424-BMP2 vectors for bone tissue engineering. These data could help guide the development of gene therapy-based protocols for bone tissue engineering.
format	article
author	Asghar Fallah Mahdieh Alipour Zahra Jamali Akbar Farjadfar Leila Roshangar Minoo Partovi Nasr Parisa Hashemi Marziyeh Aghazadeh
author_facet	Asghar Fallah Mahdieh Alipour Zahra Jamali Akbar Farjadfar Leila Roshangar Minoo Partovi Nasr Parisa Hashemi Marziyeh Aghazadeh
author_sort	Asghar Fallah
title	Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
title_short	Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
title_full	Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
title_fullStr	Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
title_full_unstemmed	Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells
title_sort	overexpression effects of mir-424 and bmp2 on the osteogenesis of wharton’s jelly-derived stem cells
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/fb379b05e0db4e6fbce6090d69de2d14
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Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

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