Identification of proteins and miRNAs that specifically bind an mRNA in vivo

The entire mRNA interactome capture has been established but identification of specific mRNA-binding proteins has been challenging. Here, the authors developed an in vivo RNA–protein crosslinking and RNA pulldown approach and characterized novel interactors of the C. elegans gld-1 mRNA, including DA...

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Autores principales:	Kathrin Theil, Koshi Imami, Nikolaus Rajewsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fb430fe1570143bcbdaa03bc4370fae0
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spelling	oai:doaj.org-article:fb430fe1570143bcbdaa03bc4370fae02021-12-02T15:35:06ZIdentification of proteins and miRNAs that specifically bind an mRNA in vivo10.1038/s41467-019-12050-72041-1723https://doaj.org/article/fb430fe1570143bcbdaa03bc4370fae02019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12050-7https://doaj.org/toc/2041-1723The entire mRNA interactome capture has been established but identification of specific mRNA-binding proteins has been challenging. Here, the authors developed an in vivo RNA–protein crosslinking and RNA pulldown approach and characterized novel interactors of the C. elegans gld-1 mRNA, including DAZ-1 and miR-84.Kathrin TheilKoshi ImamiNikolaus RajewskyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kathrin Theil Koshi Imami Nikolaus Rajewsky Identification of proteins and miRNAs that specifically bind an mRNA in vivo
description	The entire mRNA interactome capture has been established but identification of specific mRNA-binding proteins has been challenging. Here, the authors developed an in vivo RNA–protein crosslinking and RNA pulldown approach and characterized novel interactors of the C. elegans gld-1 mRNA, including DAZ-1 and miR-84.
format	article
author	Kathrin Theil Koshi Imami Nikolaus Rajewsky
author_facet	Kathrin Theil Koshi Imami Nikolaus Rajewsky
author_sort	Kathrin Theil
title	Identification of proteins and miRNAs that specifically bind an mRNA in vivo
title_short	Identification of proteins and miRNAs that specifically bind an mRNA in vivo
title_full	Identification of proteins and miRNAs that specifically bind an mRNA in vivo
title_fullStr	Identification of proteins and miRNAs that specifically bind an mRNA in vivo
title_full_unstemmed	Identification of proteins and miRNAs that specifically bind an mRNA in vivo
title_sort	identification of proteins and mirnas that specifically bind an mrna in vivo
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/fb430fe1570143bcbdaa03bc4370fae0
work_keys_str_mv	AT kathrintheil identificationofproteinsandmirnasthatspecificallybindanmrnainvivo AT koshiimami identificationofproteinsandmirnasthatspecificallybindanmrnainvivo AT nikolausrajewsky identificationofproteinsandmirnasthatspecificallybindanmrnainvivo
_version_	1718386696016363520

Identification of proteins and miRNAs that specifically bind an mRNA in vivo

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