Multiplex secretome engineering enhances recombinant protein production and purity

Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.

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Autores principales:	Stefan Kol, Daniel Ley, Tune Wulff, Marianne Decker, Johnny Arnsdorf, Sanne Schoffelen, Anders Holmgaard Hansen, Tanja Lyholm Jensen, Jahir M. Gutierrez, Austin W. T. Chiang, Helen O. Masson, Bernhard O. Palsson, Bjørn G. Voldborg, Lasse Ebdrup Pedersen, Helene Faustrup Kildegaard, Gyun Min Lee, Nathan E. Lewis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fb48699f0dc94c81a1ca056457c7fde6
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spelling	oai:doaj.org-article:fb48699f0dc94c81a1ca056457c7fde62021-12-02T15:39:28ZMultiplex secretome engineering enhances recombinant protein production and purity10.1038/s41467-020-15866-w2041-1723https://doaj.org/article/fb48699f0dc94c81a1ca056457c7fde62020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15866-whttps://doaj.org/toc/2041-1723Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.Stefan KolDaniel LeyTune WulffMarianne DeckerJohnny ArnsdorfSanne SchoffelenAnders Holmgaard HansenTanja Lyholm JensenJahir M. GutierrezAustin W. T. ChiangHelen O. MassonBernhard O. PalssonBjørn G. VoldborgLasse Ebdrup PedersenHelene Faustrup KildegaardGyun Min LeeNathan E. LewisNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis Multiplex secretome engineering enhances recombinant protein production and purity
description	Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.
format	article
author	Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis
author_facet	Stefan Kol Daniel Ley Tune Wulff Marianne Decker Johnny Arnsdorf Sanne Schoffelen Anders Holmgaard Hansen Tanja Lyholm Jensen Jahir M. Gutierrez Austin W. T. Chiang Helen O. Masson Bernhard O. Palsson Bjørn G. Voldborg Lasse Ebdrup Pedersen Helene Faustrup Kildegaard Gyun Min Lee Nathan E. Lewis
author_sort	Stefan Kol
title	Multiplex secretome engineering enhances recombinant protein production and purity
title_short	Multiplex secretome engineering enhances recombinant protein production and purity
title_full	Multiplex secretome engineering enhances recombinant protein production and purity
title_fullStr	Multiplex secretome engineering enhances recombinant protein production and purity
title_full_unstemmed	Multiplex secretome engineering enhances recombinant protein production and purity
title_sort	multiplex secretome engineering enhances recombinant protein production and purity
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/fb48699f0dc94c81a1ca056457c7fde6
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Multiplex secretome engineering enhances recombinant protein production and purity

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