Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.

<h4>Background</h4>During their transit through the female genital tract, sperm have to recognize and discriminate numerous chemical compounds. However, our current knowledge of the molecular identity of appropriate chemosensory receptor proteins in sperm is still rudimentary. Considerin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dorke Meyer, Anja Voigt, Patricia Widmayer, Heike Borth, Sandra Huebner, Andreas Breit, Susan Marschall, Martin Hrabé de Angelis, Ulrich Boehm, Wolfgang Meyerhof, Thomas Gudermann, Ingrid Boekhoff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb4ac851ee8d40d4a02f591f1fcab6f0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb4ac851ee8d40d4a02f591f1fcab6f0
record_format	dspace
spelling	oai:doaj.org-article:fb4ac851ee8d40d4a02f591f1fcab6f02021-11-18T07:26:20ZExpression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.1932-620310.1371/journal.pone.0032354https://doaj.org/article/fb4ac851ee8d40d4a02f591f1fcab6f02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22427794/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>During their transit through the female genital tract, sperm have to recognize and discriminate numerous chemical compounds. However, our current knowledge of the molecular identity of appropriate chemosensory receptor proteins in sperm is still rudimentary. Considering that members of the Tas1r family of taste receptors are able to discriminate between a broad diversity of hydrophilic chemosensory substances, the expression of taste receptors in mammalian spermatozoa was examined.<h4>Methodology/principal findings</h4>The present manuscript documents that Tas1r1 and Tas1r3, which form the functional receptor for monosodium glutamate (umami) in taste buds on the tongue, are expressed in murine and human spermatozoa, where their localization is restricted to distinct segments of the flagellum and the acrosomal cap of the sperm head. Employing a Tas1r1-deficient mCherry reporter mouse strain, we found that Tas1r1 gene deletion resulted in spermatogenic abnormalities. In addition, a significant increase in spontaneous acrosomal reaction was observed in Tas1r1 null mutant sperm whereas acrosomal secretion triggered by isolated zona pellucida or the Ca²⁺ ionophore A23187 was not different from wild-type spermatozoa. Remarkably, cytosolic Ca²⁺ levels in freshly isolated Tas1r1-deficient sperm were significantly higher compared to wild-type cells. Moreover, a significantly higher basal cAMP concentration was detected in freshly isolated Tas1r1-deficient epididymal spermatozoa, whereas upon inhibition of phosphodiesterase or sperm capacitation, the amount of cAMP was not different between both genotypes.<h4>Conclusions/significance</h4>Since Ca²⁺ and cAMP control fundamental processes during the sequential process of fertilization, we propose that the identified taste receptors and coupled signaling cascades keep sperm in a chronically quiescent state until they arrive in the vicinity of the egg - either by constitutive receptor activity and/or by tonic receptor activation by gradients of diverse chemical compounds in different compartments of the female reproductive tract.Dorke MeyerAnja VoigtPatricia WidmayerHeike BorthSandra HuebnerAndreas BreitSusan MarschallMartin Hrabé de AngelisUlrich BoehmWolfgang MeyerhofThomas GudermannIngrid BoekhoffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32354 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorke Meyer Anja Voigt Patricia Widmayer Heike Borth Sandra Huebner Andreas Breit Susan Marschall Martin Hrabé de Angelis Ulrich Boehm Wolfgang Meyerhof Thomas Gudermann Ingrid Boekhoff Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
description	<h4>Background</h4>During their transit through the female genital tract, sperm have to recognize and discriminate numerous chemical compounds. However, our current knowledge of the molecular identity of appropriate chemosensory receptor proteins in sperm is still rudimentary. Considering that members of the Tas1r family of taste receptors are able to discriminate between a broad diversity of hydrophilic chemosensory substances, the expression of taste receptors in mammalian spermatozoa was examined.<h4>Methodology/principal findings</h4>The present manuscript documents that Tas1r1 and Tas1r3, which form the functional receptor for monosodium glutamate (umami) in taste buds on the tongue, are expressed in murine and human spermatozoa, where their localization is restricted to distinct segments of the flagellum and the acrosomal cap of the sperm head. Employing a Tas1r1-deficient mCherry reporter mouse strain, we found that Tas1r1 gene deletion resulted in spermatogenic abnormalities. In addition, a significant increase in spontaneous acrosomal reaction was observed in Tas1r1 null mutant sperm whereas acrosomal secretion triggered by isolated zona pellucida or the Ca²⁺ ionophore A23187 was not different from wild-type spermatozoa. Remarkably, cytosolic Ca²⁺ levels in freshly isolated Tas1r1-deficient sperm were significantly higher compared to wild-type cells. Moreover, a significantly higher basal cAMP concentration was detected in freshly isolated Tas1r1-deficient epididymal spermatozoa, whereas upon inhibition of phosphodiesterase or sperm capacitation, the amount of cAMP was not different between both genotypes.<h4>Conclusions/significance</h4>Since Ca²⁺ and cAMP control fundamental processes during the sequential process of fertilization, we propose that the identified taste receptors and coupled signaling cascades keep sperm in a chronically quiescent state until they arrive in the vicinity of the egg - either by constitutive receptor activity and/or by tonic receptor activation by gradients of diverse chemical compounds in different compartments of the female reproductive tract.
format	article
author	Dorke Meyer Anja Voigt Patricia Widmayer Heike Borth Sandra Huebner Andreas Breit Susan Marschall Martin Hrabé de Angelis Ulrich Boehm Wolfgang Meyerhof Thomas Gudermann Ingrid Boekhoff
author_facet	Dorke Meyer Anja Voigt Patricia Widmayer Heike Borth Sandra Huebner Andreas Breit Susan Marschall Martin Hrabé de Angelis Ulrich Boehm Wolfgang Meyerhof Thomas Gudermann Ingrid Boekhoff
author_sort	Dorke Meyer
title	Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
title_short	Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
title_full	Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
title_fullStr	Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
title_full_unstemmed	Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
title_sort	expression of tas1 taste receptors in mammalian spermatozoa: functional role of tas1r1 in regulating basal ca²⁺ and camp concentrations in spermatozoa.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fb4ac851ee8d40d4a02f591f1fcab6f0
work_keys_str_mv	AT dorkemeyer expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT anjavoigt expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT patriciawidmayer expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT heikeborth expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT sandrahuebner expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT andreasbreit expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT susanmarschall expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT martinhrabedeangelis expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT ulrichboehm expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT wolfgangmeyerhof expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT thomasgudermann expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa AT ingridboekhoff expressionoftas1tastereceptorsinmammalianspermatozoafunctionalroleoftas1r1inregulatingbasalca2andcampconcentrationsinspermatozoa
_version_	1718423511946493952

Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.

Ejemplares similares