UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats

Context: Melastoma dodecandrum Lour. (Melastomataceae) is a traditional Chinese medicine. This is the first study to report a protective effect of the ethanol extract from M. dodecandrum (MDE) in type 2 diabetic (T2DM) rats. Objective: To investigate the therapeutic mechanism of MDE in T2DM rats. Ma...

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Autores principales:	Jingyu Weng, Jingkai Zhou, Liqing Liang, Li Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	flavonoid blood lipid blood glucose oxidative stress streptozotocin Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/fb649969adb344e883c410d3194a5c72
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spelling	oai:doaj.org-article:fb649969adb344e883c410d3194a5c722021-11-17T14:21:56ZUHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats1388-02091744-511610.1080/13880209.2019.1693605https://doaj.org/article/fb649969adb344e883c410d3194a5c722019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1693605https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Melastoma dodecandrum Lour. (Melastomataceae) is a traditional Chinese medicine. This is the first study to report a protective effect of the ethanol extract from M. dodecandrum (MDE) in type 2 diabetic (T2DM) rats. Objective: To investigate the therapeutic mechanism of MDE in T2DM rats. Materials and methods: Sprague-Dawley rats were fed a high-fat diet for 6 consecutive weeks, followed by intraperitoneal injection of streptozotocin (STZ) (30 mg/kg) to induce diabetes. T2DM rats were divided into untreated diabetic, metformin-treated and MDE-treated groups. Additionally, normal rats without treatment served as the control group (n = 6). Metformin (250 mg/kg) and MDE (600 mg/kg) were intragastrically administered to T2DM rats for 5 consecutive weeks. Serum samples were evaluated via ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS), followed by principal components analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Results: The 17 identified potential biomarkers were mainly involved in lipid, amino acid, arachidonic acid, taurine and nicotinic acid metabolism. MDE also significantly reduced the level of fasting blood glucose (FBG), oral glucose tolerance, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and urea nitrogen (BUN) in T2DM rats. The high-density lipoprotein (HDL), serum creatinine (Scr), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) levels were elevated in MDE-treated group. Discussion and conclusion: MDE possesses substantial antidiabetic activity, especially in lipid disorder regulation. This suggests that the use of MDE can be generalized to broader pharmacological studies, such as obesity and hyperlipidaemia.Jingyu WengJingkai ZhouLiqing LiangLi LiTaylor & Francis Grouparticleflavonoidblood lipidblood glucoseoxidative stressstreptozotocinTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 807-816 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	flavonoid blood lipid blood glucose oxidative stress streptozotocin Therapeutics. Pharmacology RM1-950
spellingShingle	flavonoid blood lipid blood glucose oxidative stress streptozotocin Therapeutics. Pharmacology RM1-950 Jingyu Weng Jingkai Zhou Liqing Liang Li Li UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
description	Context: Melastoma dodecandrum Lour. (Melastomataceae) is a traditional Chinese medicine. This is the first study to report a protective effect of the ethanol extract from M. dodecandrum (MDE) in type 2 diabetic (T2DM) rats. Objective: To investigate the therapeutic mechanism of MDE in T2DM rats. Materials and methods: Sprague-Dawley rats were fed a high-fat diet for 6 consecutive weeks, followed by intraperitoneal injection of streptozotocin (STZ) (30 mg/kg) to induce diabetes. T2DM rats were divided into untreated diabetic, metformin-treated and MDE-treated groups. Additionally, normal rats without treatment served as the control group (n = 6). Metformin (250 mg/kg) and MDE (600 mg/kg) were intragastrically administered to T2DM rats for 5 consecutive weeks. Serum samples were evaluated via ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS), followed by principal components analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Results: The 17 identified potential biomarkers were mainly involved in lipid, amino acid, arachidonic acid, taurine and nicotinic acid metabolism. MDE also significantly reduced the level of fasting blood glucose (FBG), oral glucose tolerance, insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and urea nitrogen (BUN) in T2DM rats. The high-density lipoprotein (HDL), serum creatinine (Scr), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) levels were elevated in MDE-treated group. Discussion and conclusion: MDE possesses substantial antidiabetic activity, especially in lipid disorder regulation. This suggests that the use of MDE can be generalized to broader pharmacological studies, such as obesity and hyperlipidaemia.
format	article
author	Jingyu Weng Jingkai Zhou Liqing Liang Li Li
author_facet	Jingyu Weng Jingkai Zhou Liqing Liang Li Li
author_sort	Jingyu Weng
title	UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
title_short	UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
title_full	UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
title_fullStr	UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
title_full_unstemmed	UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats
title_sort	uhplc/qtof-ms-based metabolomics reveal the effect of melastoma dodecandrum extract in type 2 diabetic rats
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/fb649969adb344e883c410d3194a5c72
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UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats

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