N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont

Abstract Mangrove forests are among the most productive and diverse ecosystems on the planet, despite limited nitrogen (N) availability. Under such conditions, animal-microbe associations (holobionts) are often key to ecosystem functioning. Here, we investigated the role of fiddler crabs and their c...

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Autores principales:	Mindaugas Zilius, Stefano Bonaglia, Elias Broman, Vitor Gonsalez Chiozzini, Aurelija Samuiloviene, Francisco J. A. Nascimento, Ulisse Cardini, Marco Bartoli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb67db95c4d043baae424f1939596b1f
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spelling	oai:doaj.org-article:fb67db95c4d043baae424f1939596b1f2021-12-02T16:45:53ZN2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont10.1038/s41598-020-70834-02045-2322https://doaj.org/article/fb67db95c4d043baae424f1939596b1f2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70834-0https://doaj.org/toc/2045-2322Abstract Mangrove forests are among the most productive and diverse ecosystems on the planet, despite limited nitrogen (N) availability. Under such conditions, animal-microbe associations (holobionts) are often key to ecosystem functioning. Here, we investigated the role of fiddler crabs and their carapace-associated microbial biofilm as hotspots of microbial N transformations and sources of N within the mangrove ecosystem. 16S rRNA gene and metagenomic sequencing provided evidence of a microbial biofilm dominated by Cyanobacteria, Alphaproteobacteria, Actinobacteria, and Bacteroidota with a community encoding both aerobic and anaerobic pathways of the N cycle. Dinitrogen (N2) fixation was among the most commonly predicted process. Net N fluxes between the biofilm-covered crabs and the water and microbial N transformation rates in suspended biofilm slurries portray these holobionts as a net N2 sink, with N2 fixation exceeding N losses, and as a significant source of ammonium and dissolved organic N to the surrounding environment. N stable isotope natural abundances of fiddler crab carapace-associated biofilms were within the range expected for fixed N, further suggesting active microbial N2 fixation. These results extend our knowledge on the diversity of invertebrate-microbe associations, and provide a clear example of how animal microbiota can mediate a plethora of essential biogeochemical processes in mangrove ecosystems.Mindaugas ZiliusStefano BonagliaElias BromanVitor Gonsalez ChiozziniAurelija SamuilovieneFrancisco J. A. NascimentoUlisse CardiniMarco BartoliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mindaugas Zilius Stefano Bonaglia Elias Broman Vitor Gonsalez Chiozzini Aurelija Samuiloviene Francisco J. A. Nascimento Ulisse Cardini Marco Bartoli N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
description	Abstract Mangrove forests are among the most productive and diverse ecosystems on the planet, despite limited nitrogen (N) availability. Under such conditions, animal-microbe associations (holobionts) are often key to ecosystem functioning. Here, we investigated the role of fiddler crabs and their carapace-associated microbial biofilm as hotspots of microbial N transformations and sources of N within the mangrove ecosystem. 16S rRNA gene and metagenomic sequencing provided evidence of a microbial biofilm dominated by Cyanobacteria, Alphaproteobacteria, Actinobacteria, and Bacteroidota with a community encoding both aerobic and anaerobic pathways of the N cycle. Dinitrogen (N2) fixation was among the most commonly predicted process. Net N fluxes between the biofilm-covered crabs and the water and microbial N transformation rates in suspended biofilm slurries portray these holobionts as a net N2 sink, with N2 fixation exceeding N losses, and as a significant source of ammonium and dissolved organic N to the surrounding environment. N stable isotope natural abundances of fiddler crab carapace-associated biofilms were within the range expected for fixed N, further suggesting active microbial N2 fixation. These results extend our knowledge on the diversity of invertebrate-microbe associations, and provide a clear example of how animal microbiota can mediate a plethora of essential biogeochemical processes in mangrove ecosystems.
format	article
author	Mindaugas Zilius Stefano Bonaglia Elias Broman Vitor Gonsalez Chiozzini Aurelija Samuiloviene Francisco J. A. Nascimento Ulisse Cardini Marco Bartoli
author_facet	Mindaugas Zilius Stefano Bonaglia Elias Broman Vitor Gonsalez Chiozzini Aurelija Samuiloviene Francisco J. A. Nascimento Ulisse Cardini Marco Bartoli
author_sort	Mindaugas Zilius
title	N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
title_short	N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
title_full	N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
title_fullStr	N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
title_full_unstemmed	N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
title_sort	n2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/fb67db95c4d043baae424f1939596b1f
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_version_	1718383493201788928

N2 fixation dominates nitrogen cycling in a mangrove fiddler crab holobiont

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