Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC

Abstract Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. In recent years, epitranscriptomics has been an active area of research. However, a major issue has been the development of sequencing methods to map transcriptome-wide RN...

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Autores principales:	Takahito Ohshiro, Masamitsu Konno, Ayumu Asai, Yuki Komoto, Akira Yamagata, Yuichiro Doki, Hidetoshi Eguchi, Ken Ofusa, Masateru Taniguchi, Hideshi Ishii
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb68851eda5a4b55ad2abd1b0b484d75
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spelling	oai:doaj.org-article:fb68851eda5a4b55ad2abd1b0b484d752021-12-02T18:51:28ZSingle-molecule RNA sequencing for simultaneous detection of m6A and 5mC10.1038/s41598-021-98805-z2045-2322https://doaj.org/article/fb68851eda5a4b55ad2abd1b0b484d752021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98805-zhttps://doaj.org/toc/2045-2322Abstract Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. In recent years, epitranscriptomics has been an active area of research. However, a major issue has been the development of sequencing methods to map transcriptome-wide RNA base modifications. We have proposed a single-molecule quantum sequencer for mapping RNA base modifications in microRNAs (miRNAs), such as N6-methyladenosine (m6A) or 5-methylcytidine (5mC), which are related to cancer cell propagation and suppression. Here, we investigated 5mC and m6A in hsa-miR-200c-5p extracted from colorectal cancer cells and determined their methylation sites and rates; the data were comparable to those determined by mass spectrometry. Furthermore, we evaluated the methylation ratio of cytidine and adenosine at each site in the sequences and its relationship. These results suggest that the methylation ratio of cytidine and adenosine is facilitated by the presence of vicinal methylation. Our work provides a robust new tool for sequencing various types of RNA base modifications in their RNA context.Takahito OhshiroMasamitsu KonnoAyumu AsaiYuki KomotoAkira YamagataYuichiro DokiHidetoshi EguchiKen OfusaMasateru TaniguchiHideshi IshiiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahito Ohshiro Masamitsu Konno Ayumu Asai Yuki Komoto Akira Yamagata Yuichiro Doki Hidetoshi Eguchi Ken Ofusa Masateru Taniguchi Hideshi Ishii Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
description	Abstract Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. In recent years, epitranscriptomics has been an active area of research. However, a major issue has been the development of sequencing methods to map transcriptome-wide RNA base modifications. We have proposed a single-molecule quantum sequencer for mapping RNA base modifications in microRNAs (miRNAs), such as N6-methyladenosine (m6A) or 5-methylcytidine (5mC), which are related to cancer cell propagation and suppression. Here, we investigated 5mC and m6A in hsa-miR-200c-5p extracted from colorectal cancer cells and determined their methylation sites and rates; the data were comparable to those determined by mass spectrometry. Furthermore, we evaluated the methylation ratio of cytidine and adenosine at each site in the sequences and its relationship. These results suggest that the methylation ratio of cytidine and adenosine is facilitated by the presence of vicinal methylation. Our work provides a robust new tool for sequencing various types of RNA base modifications in their RNA context.
format	article
author	Takahito Ohshiro Masamitsu Konno Ayumu Asai Yuki Komoto Akira Yamagata Yuichiro Doki Hidetoshi Eguchi Ken Ofusa Masateru Taniguchi Hideshi Ishii
author_facet	Takahito Ohshiro Masamitsu Konno Ayumu Asai Yuki Komoto Akira Yamagata Yuichiro Doki Hidetoshi Eguchi Ken Ofusa Masateru Taniguchi Hideshi Ishii
author_sort	Takahito Ohshiro
title	Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
title_short	Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
title_full	Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
title_fullStr	Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
title_full_unstemmed	Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
title_sort	single-molecule rna sequencing for simultaneous detection of m6a and 5mc
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fb68851eda5a4b55ad2abd1b0b484d75
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Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC

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