Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic Staphylococcus Species Based on Novel Molecular Target Genes

Coagulase-positive Staphylococcus aureus is a foodborne pathogen considered one of the causes of food-related disease outbreaks. Like S. aureus, Staphylococcus capitis, Staphylococcus caprae, and S. epidermidis</...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eiseul Kim, Seung-Min Yang, Ji-Eun Won, Da-Young Kim, Da-Som Kim, Hae-Yeong Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> <i>Staphylococcus capitis</i> <i>Staphylococcus caprae</i> <i>Staphylococcus epidermidis</i> real-time PCR pan-genome analysis Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/fb6b32542d5842ebb4a7fead2b6e46cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb6b32542d5842ebb4a7fead2b6e46cb
record_format	dspace
spelling	oai:doaj.org-article:fb6b32542d5842ebb4a7fead2b6e46cb2021-11-25T17:36:18ZReal-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes10.3390/foods101128392304-8158https://doaj.org/article/fb6b32542d5842ebb4a7fead2b6e46cb2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2839https://doaj.org/toc/2304-8158Coagulase-positive <i>Staphylococcus aureus</i> is a foodborne pathogen considered one of the causes of food-related disease outbreaks. Like <i>S. aureus</i>, <i>Staphylococcus capitis</i>, <i>Staphylococcus caprae</i>, and <i>S. epidermidis</i> are opportunistic pathogens causing clinical infections and food contamination. The objective of our study was to develop a rapid, accurate, and monitoring technique to detect four <i>Staphylococcus</i> species in food. Four novel molecular targets (GntR family transcriptional regulator for <i>S. aureus</i>, phosphomannomutase for <i>S. epidermidis</i>, FAD-dependent urate hydroxylase for <i>S. capitis</i>, and Gram-positive signal peptide protein for <i>S. caprae</i>) were mined based on pan-genome analysis. Primers targeting molecular target genes showed 100% specificity for 100 non-target reference strains. The detection limit in pure cultures and artificially contaminated food samples was 10<sup>2</sup> colony-forming unit/mL for <i>S. aureus</i>, <i>S. capitis</i>, <i>S. caprae</i>, and <i>S. epidermidis</i>. Moreover, real-time polymerase chain reaction successfully detected strains isolated from various food matrices. Thus, our method allows an accurate and rapid monitoring of <i>Staphylococcus</i> species and may help control staphylococcal contamination of food.Eiseul KimSeung-Min YangJi-Eun WonDa-Young KimDa-Som KimHae-Yeong KimMDPI AGarticle<i>Staphylococcus aureus</i><i>Staphylococcus capitis</i><i>Staphylococcus caprae</i><i>Staphylococcus epidermidis</i>real-time PCRpan-genome analysisChemical technologyTP1-1185ENFoods, Vol 10, Iss 2839, p 2839 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> <i>Staphylococcus capitis</i> <i>Staphylococcus caprae</i> <i>Staphylococcus epidermidis</i> real-time PCR pan-genome analysis Chemical technology TP1-1185
spellingShingle	<i>Staphylococcus aureus</i> <i>Staphylococcus capitis</i> <i>Staphylococcus caprae</i> <i>Staphylococcus epidermidis</i> real-time PCR pan-genome analysis Chemical technology TP1-1185 Eiseul Kim Seung-Min Yang Ji-Eun Won Da-Young Kim Da-Som Kim Hae-Yeong Kim Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
description	Coagulase-positive <i>Staphylococcus aureus</i> is a foodborne pathogen considered one of the causes of food-related disease outbreaks. Like <i>S. aureus</i>, <i>Staphylococcus capitis</i>, <i>Staphylococcus caprae</i>, and <i>S. epidermidis</i> are opportunistic pathogens causing clinical infections and food contamination. The objective of our study was to develop a rapid, accurate, and monitoring technique to detect four <i>Staphylococcus</i> species in food. Four novel molecular targets (GntR family transcriptional regulator for <i>S. aureus</i>, phosphomannomutase for <i>S. epidermidis</i>, FAD-dependent urate hydroxylase for <i>S. capitis</i>, and Gram-positive signal peptide protein for <i>S. caprae</i>) were mined based on pan-genome analysis. Primers targeting molecular target genes showed 100% specificity for 100 non-target reference strains. The detection limit in pure cultures and artificially contaminated food samples was 10<sup>2</sup> colony-forming unit/mL for <i>S. aureus</i>, <i>S. capitis</i>, <i>S. caprae</i>, and <i>S. epidermidis</i>. Moreover, real-time polymerase chain reaction successfully detected strains isolated from various food matrices. Thus, our method allows an accurate and rapid monitoring of <i>Staphylococcus</i> species and may help control staphylococcal contamination of food.
format	article
author	Eiseul Kim Seung-Min Yang Ji-Eun Won Da-Young Kim Da-Som Kim Hae-Yeong Kim
author_facet	Eiseul Kim Seung-Min Yang Ji-Eun Won Da-Young Kim Da-Som Kim Hae-Yeong Kim
author_sort	Eiseul Kim
title	Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
title_short	Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
title_full	Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
title_fullStr	Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
title_full_unstemmed	Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes
title_sort	real-time pcr method for the rapid detection and quantification of pathogenic <i>staphylococcus</i> species based on novel molecular target genes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fb6b32542d5842ebb4a7fead2b6e46cb
work_keys_str_mv	AT eiseulkim realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes AT seungminyang realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes AT jieunwon realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes AT dayoungkim realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes AT dasomkim realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes AT haeyeongkim realtimepcrmethodfortherapiddetectionandquantificationofpathogenicistaphylococcusispeciesbasedonnovelmoleculartargetgenes
_version_	1718412203661459456

Real-Time PCR Method for the Rapid Detection and Quantification of Pathogenic <i>Staphylococcus</i> Species Based on Novel Molecular Target Genes

Ejemplares similares