Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma

Abstract Colorectal carcinoma (CRC) is associated with significant morbidity and mortality worldwide. Cytokeratins (CKs) are widely expressed in various types of carcinomas, whereas in CRC it is usually CK7 − and CK20 + . A subset of CRCs is CK7 + . This study aims to determine the prevalence of CK7...

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Autores principales:	Jan Hrudka, Hana Fišerová, Karolína Jelínková, Radoslav Matěj, Petr Waldauf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb6edba5a7244cdba6cb101c64dd69d3
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spelling	oai:doaj.org-article:fb6edba5a7244cdba6cb101c64dd69d32021-12-02T19:12:35ZCytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma10.1038/s41598-021-97480-42045-2322https://doaj.org/article/fb6edba5a7244cdba6cb101c64dd69d32021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97480-4https://doaj.org/toc/2045-2322Abstract Colorectal carcinoma (CRC) is associated with significant morbidity and mortality worldwide. Cytokeratins (CKs) are widely expressed in various types of carcinomas, whereas in CRC it is usually CK7 − and CK20 + . A subset of CRCs is CK7 + . This study aims to determine the prevalence of CK7 expression in CRC and its impact on overall survival. We analyzed 300 randomly selected surgically treated CRC cases using paraffin embedded tumor tissue samples and evaluated CK7 and CK20 expression using the tissue microarray method. Tumors with positivity > 10% and > 25% of tumor cells were considered CK7 and CK20 positive, respectively. Expression of both CKs and several clinical-pathological variables (stage, grade, laterality, mismatch-repair/MMR status) were evaluated using patient follow up data (Kaplan–Meier analysis of cancer-specific survival (CSS)). Significant results include shorter CSS (restricted mean 4.98 vs. 7.74 years, P = 0.007) and 5-year survival (29.4% vs. 64.6%, P = 0.0221) in CK7 + tumors compared to CK7 − tumors, respectively; without significant association with grade, stage or right-sided location. These results were significant in a multivariate analysis. CK20 + tumors are more frequently MMR-proficient and left-sided. MMR-deficient tumors are more frequently right-sided and had longer survival. CK7 expression, right-sided location (rmean CSS 6.83 vs. 8.0 years, P = 0.043), MMR-proficiency (rmean CSS 7.41 vs. 9.32 years, P = 0.012), and UICC stages III + IV (rmean CSS 6.03 vs. 8.92 years, P < 0.001) of the tumor correlated with negative prognostic outcomes, whereas the most significant results concern stage and CK7 positivity. The result concerning negative prognostic role of CK7 differs from those obtained by several previous studies focused on this topic.Jan HrudkaHana FišerováKarolína JelínkováRadoslav MatějPetr WaldaufNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jan Hrudka Hana Fišerová Karolína Jelínková Radoslav Matěj Petr Waldauf Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
description	Abstract Colorectal carcinoma (CRC) is associated with significant morbidity and mortality worldwide. Cytokeratins (CKs) are widely expressed in various types of carcinomas, whereas in CRC it is usually CK7 − and CK20 + . A subset of CRCs is CK7 + . This study aims to determine the prevalence of CK7 expression in CRC and its impact on overall survival. We analyzed 300 randomly selected surgically treated CRC cases using paraffin embedded tumor tissue samples and evaluated CK7 and CK20 expression using the tissue microarray method. Tumors with positivity > 10% and > 25% of tumor cells were considered CK7 and CK20 positive, respectively. Expression of both CKs and several clinical-pathological variables (stage, grade, laterality, mismatch-repair/MMR status) were evaluated using patient follow up data (Kaplan–Meier analysis of cancer-specific survival (CSS)). Significant results include shorter CSS (restricted mean 4.98 vs. 7.74 years, P = 0.007) and 5-year survival (29.4% vs. 64.6%, P = 0.0221) in CK7 + tumors compared to CK7 − tumors, respectively; without significant association with grade, stage or right-sided location. These results were significant in a multivariate analysis. CK20 + tumors are more frequently MMR-proficient and left-sided. MMR-deficient tumors are more frequently right-sided and had longer survival. CK7 expression, right-sided location (rmean CSS 6.83 vs. 8.0 years, P = 0.043), MMR-proficiency (rmean CSS 7.41 vs. 9.32 years, P = 0.012), and UICC stages III + IV (rmean CSS 6.03 vs. 8.92 years, P < 0.001) of the tumor correlated with negative prognostic outcomes, whereas the most significant results concern stage and CK7 positivity. The result concerning negative prognostic role of CK7 differs from those obtained by several previous studies focused on this topic.
format	article
author	Jan Hrudka Hana Fišerová Karolína Jelínková Radoslav Matěj Petr Waldauf
author_facet	Jan Hrudka Hana Fišerová Karolína Jelínková Radoslav Matěj Petr Waldauf
author_sort	Jan Hrudka
title	Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
title_short	Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
title_full	Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
title_fullStr	Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
title_full_unstemmed	Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
title_sort	cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fb6edba5a7244cdba6cb101c64dd69d3
work_keys_str_mv	AT janhrudka cytokeratin7expressionasapredictorofanunfavorableprognosisincolorectalcarcinoma AT hanafiserova cytokeratin7expressionasapredictorofanunfavorableprognosisincolorectalcarcinoma AT karolinajelinkova cytokeratin7expressionasapredictorofanunfavorableprognosisincolorectalcarcinoma AT radoslavmatej cytokeratin7expressionasapredictorofanunfavorableprognosisincolorectalcarcinoma AT petrwaldauf cytokeratin7expressionasapredictorofanunfavorableprognosisincolorectalcarcinoma
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Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma

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