Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses

Crayfish are a keystone species of freshwater ecosystems and a successful invasive species. However, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of the invasive signal crayfish (<i>Pacifastacus leniusculus</i>) and to elucidate...

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Autores principales:	Katarina Bačnik, Denis Kutnjak, Silvija Černi, Ana Bielen, Sandra Hudina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	signal crayfish virome RNA viruses invasive alien species invasion range high-throughput sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fb76138894dd47bda9aae4fa831a3b77
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spelling	oai:doaj.org-article:fb76138894dd47bda9aae4fa831a3b772021-11-25T19:13:51ZVirome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses10.3390/v131122591999-4915https://doaj.org/article/fb76138894dd47bda9aae4fa831a3b772021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2259https://doaj.org/toc/1999-4915Crayfish are a keystone species of freshwater ecosystems and a successful invasive species. However, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of the invasive signal crayfish (<i>Pacifastacus leniusculus</i>) and to elucidate the potential differences in viral composition and abundance along its invasion range in the Korana River, Croatia. By the high-throughput sequencing of ribosomal RNA, depleted total RNA isolated from the crayfish hepatopancreas, and subsequent sequence data analysis, we identified novel and divergent RNA viruses, including signal crayfish-associated reo-like, hepe-like, toti-like, and picorna-like viruses, phylogenetically related to viruses previously associated with crustacean hosts. The patterns of reads abundance and calculated nucleotide diversities of the detected viral sequences varied along the invasion range. This could indicate the possible influence of different factors and processes on signal crayfish virome composition: e.g., the differences in signal crayfish population density, the non-random dispersal of host individuals from the core to the invasion fronts, and the transfer of viruses from the native co-occurring and phylogenetically related crayfish species. The study reveals a high, previously undiscovered diversity of divergent RNA viruses associated with signal crayfish, and sets foundations for understanding the potential risk of virus transmissions as a result of this invader’s dispersal.Katarina BačnikDenis KutnjakSilvija ČerniAna BielenSandra HudinaMDPI AGarticlesignal crayfish viromeRNA virusesinvasive alien speciesinvasion rangehigh-throughput sequencingMicrobiologyQR1-502ENViruses, Vol 13, Iss 2259, p 2259 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	signal crayfish virome RNA viruses invasive alien species invasion range high-throughput sequencing Microbiology QR1-502
spellingShingle	signal crayfish virome RNA viruses invasive alien species invasion range high-throughput sequencing Microbiology QR1-502 Katarina Bačnik Denis Kutnjak Silvija Černi Ana Bielen Sandra Hudina Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
description	Crayfish are a keystone species of freshwater ecosystems and a successful invasive species. However, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of the invasive signal crayfish (<i>Pacifastacus leniusculus</i>) and to elucidate the potential differences in viral composition and abundance along its invasion range in the Korana River, Croatia. By the high-throughput sequencing of ribosomal RNA, depleted total RNA isolated from the crayfish hepatopancreas, and subsequent sequence data analysis, we identified novel and divergent RNA viruses, including signal crayfish-associated reo-like, hepe-like, toti-like, and picorna-like viruses, phylogenetically related to viruses previously associated with crustacean hosts. The patterns of reads abundance and calculated nucleotide diversities of the detected viral sequences varied along the invasion range. This could indicate the possible influence of different factors and processes on signal crayfish virome composition: e.g., the differences in signal crayfish population density, the non-random dispersal of host individuals from the core to the invasion fronts, and the transfer of viruses from the native co-occurring and phylogenetically related crayfish species. The study reveals a high, previously undiscovered diversity of divergent RNA viruses associated with signal crayfish, and sets foundations for understanding the potential risk of virus transmissions as a result of this invader’s dispersal.
format	article
author	Katarina Bačnik Denis Kutnjak Silvija Černi Ana Bielen Sandra Hudina
author_facet	Katarina Bačnik Denis Kutnjak Silvija Černi Ana Bielen Sandra Hudina
author_sort	Katarina Bačnik
title	Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
title_short	Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
title_full	Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
title_fullStr	Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
title_full_unstemmed	Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses
title_sort	virome analysis of signal crayfish (<i>pacifastacus leniusculus</i>) along its invasion range reveals diverse and divergent rna viruses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fb76138894dd47bda9aae4fa831a3b77
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Virome Analysis of Signal Crayfish (<i>Pacifastacus leniusculus</i>) along Its Invasion Range Reveals Diverse and Divergent RNA Viruses

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