Nuocytes from mesenteric lymph node promote allergic responses in a mouse model

Introduction: Nuocytes play an important role in Type 2 immunity. However, the contribution of ILC2s to allergic rhinitis remains to be clearly elucidated. Objective: To evaluate the role of nuocytes from mesenteric lymph node on allergic responses in mice. Methods: After intraperitoneal administrat...

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Autores principales: Lin Lin, Xinyue Tang, Zheng Chen, Jinjin Wei, Fei Dai, Guangbin Sun
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Lenguaje:EN
Publicado: Elsevier 2021
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spelling oai:doaj.org-article:fb78e58d6ec2469cb34611c61138b2402021-11-06T04:23:33ZNuocytes from mesenteric lymph node promote allergic responses in a mouse model1808-869410.1016/j.bjorl.2019.12.010https://doaj.org/article/fb78e58d6ec2469cb34611c61138b2402021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1808869420300240https://doaj.org/toc/1808-8694Introduction: Nuocytes play an important role in Type 2 immunity. However, the contribution of ILC2s to allergic rhinitis remains to be clearly elucidated. Objective: To evaluate the role of nuocytes from mesenteric lymph node on allergic responses in mice. Methods: After intraperitoneal administration of interleukin IL-25 and IL-33 to wild-type and Il17br−/−Il1rl1−/− double-deficient mice, nuocytes were purified from the the nasal-associated lymphoid tissue and mesenteric lymph nodes. Then, we assessed productions of IL-5 and IL-13 in nuocytes’ cultures. Finally, we adoptively transferred the mesenteric lymph node-derived nuocytes from wild-type and Il17br−/−Il1rl1−/− mice to the murine model of allergic rhinitis to evaluate their roles in nasal allergic responses. Results: We showed that nuocytes in the mesenteric lymph nodes of wild-type mice were upregulated after application of IL-25 and IL-33, and were induced to produce IL-5 and IL-13. Numbers of sneezing and nasal rubbing as well as eosinophils were all enhanced after the adoptive transfer of wild-type nuocytes. Concentrations of IL-5, IL-13, IL-25 and IL-33 in nasal lavage fluid of allergic mice were also increased. However, nuocytes fromIl17br−/−Il1rl1−/− mice did not increase sneezing and nasal rubbing and eosinophilia, and upregulate the above cytokines in the nasal lavage fluid. Conclusion: The findings demonstrate that nuocytes from the mesenteric lymph nodes of wild-type mice promote allergic responses in a mouse model.Lin LinXinyue TangZheng ChenJinjin WeiFei DaiGuangbin SunElsevierarticleLymphoid cellsLymph nodeAllergic rhinitisMiceAnimal modelsOtorhinolaryngologyRF1-547ENBrazilian Journal of Otorhinolaryngology, Vol 87, Iss 6, Pp 661-670 (2021)
institution DOAJ
collection DOAJ
language EN
topic Lymphoid cells
Lymph node
Allergic rhinitis
Mice
Animal models
Otorhinolaryngology
RF1-547
spellingShingle Lymphoid cells
Lymph node
Allergic rhinitis
Mice
Animal models
Otorhinolaryngology
RF1-547
Lin Lin
Xinyue Tang
Zheng Chen
Jinjin Wei
Fei Dai
Guangbin Sun
Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
description Introduction: Nuocytes play an important role in Type 2 immunity. However, the contribution of ILC2s to allergic rhinitis remains to be clearly elucidated. Objective: To evaluate the role of nuocytes from mesenteric lymph node on allergic responses in mice. Methods: After intraperitoneal administration of interleukin IL-25 and IL-33 to wild-type and Il17br−/−Il1rl1−/− double-deficient mice, nuocytes were purified from the the nasal-associated lymphoid tissue and mesenteric lymph nodes. Then, we assessed productions of IL-5 and IL-13 in nuocytes’ cultures. Finally, we adoptively transferred the mesenteric lymph node-derived nuocytes from wild-type and Il17br−/−Il1rl1−/− mice to the murine model of allergic rhinitis to evaluate their roles in nasal allergic responses. Results: We showed that nuocytes in the mesenteric lymph nodes of wild-type mice were upregulated after application of IL-25 and IL-33, and were induced to produce IL-5 and IL-13. Numbers of sneezing and nasal rubbing as well as eosinophils were all enhanced after the adoptive transfer of wild-type nuocytes. Concentrations of IL-5, IL-13, IL-25 and IL-33 in nasal lavage fluid of allergic mice were also increased. However, nuocytes fromIl17br−/−Il1rl1−/− mice did not increase sneezing and nasal rubbing and eosinophilia, and upregulate the above cytokines in the nasal lavage fluid. Conclusion: The findings demonstrate that nuocytes from the mesenteric lymph nodes of wild-type mice promote allergic responses in a mouse model.
format article
author Lin Lin
Xinyue Tang
Zheng Chen
Jinjin Wei
Fei Dai
Guangbin Sun
author_facet Lin Lin
Xinyue Tang
Zheng Chen
Jinjin Wei
Fei Dai
Guangbin Sun
author_sort Lin Lin
title Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
title_short Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
title_full Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
title_fullStr Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
title_full_unstemmed Nuocytes from mesenteric lymph node promote allergic responses in a mouse model
title_sort nuocytes from mesenteric lymph node promote allergic responses in a mouse model
publisher Elsevier
publishDate 2021
url https://doaj.org/article/fb78e58d6ec2469cb34611c61138b240
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AT xinyuetang nuocytesfrommesentericlymphnodepromoteallergicresponsesinamousemodel
AT zhengchen nuocytesfrommesentericlymphnodepromoteallergicresponsesinamousemodel
AT jinjinwei nuocytesfrommesentericlymphnodepromoteallergicresponsesinamousemodel
AT feidai nuocytesfrommesentericlymphnodepromoteallergicresponsesinamousemodel
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